Available from stock

Neutral Red 5

SKU: N0249

Category: Non Fluorescent Detection & Chromogene Detection

Synonyms: C.I. 50040 , 3-Amino-7-dimethylamino-2-methylphenazine hydrochloride , Basic Red 5 , Toluylene red; 553-24-2
CAS-Number: C₁₅H₁₇CIN₄
Molecular Formula: 288.78
Molecular Weight

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Product Data
Handling
Safety Information
Details

Specifications

Purity: for microscopy
Appearance: Red to dark red powder
Identity: 1H-NMR

Properties

Solvents: water (10 mg/ml)

Shipping: AMBIENT
Short Term Storage: RT
Long Term Storage: RT
Handling Advice: Protect from light and moisture.
Use / Stability: Stable for at least 2 years after receipt when stored at RT.

Transportation: Not dangerous goods

Description: Neutral red acts as a cellular pH indicator, changing from red to yellow between pH 6.8 and 8.0. More important, it is a dye used for staining in histology. It stains lysosomes red and is used as a general stain in histology, as a counterstain in combination with other dyes, and for many staining methods. It is applied in histology for nuclear staining, fluorescence staining, polychrome staining or for counter staining in Gram staining. It has also been incorporated in investigation of viruses, as a pH indicator, determination of DNA with optical and electrochemical methods, evalutaion of synthetic and naturally derived biomaterials. In microbiology, it is used in the MacConkey agar to differentiate bacteria for lactose fermentation. Neutral red is often used as a vital stain. Live cells incorporate neutral red into their lysosomes. As cells begin to die, their ability to incorporate neutral red diminishes. Thus, loss of neutral red uptake corresponds to loss of cell viability. The compound once taken up by cells can be used to acertain cell viability with a excitation maximum at 470nm and emission maximum at 580nm. Neutral red is added to some growth media for bacterial and cell cultures. Neutral red can be electrochemically polymerized and used as a redox mediator.
Smiles: CC1=C(N)C=C(NC(C2=N3)=C/C(C=C2)=[N+](C)/C)C3=C1.[Cl-]
InChi Key: GIBXNAFNURIQII-UHFFFAOYSA-N
References: (1) J. Winckler, Red. Prog. Histochem. Cytochem. 6, 1 (1974) , (2) P. Antal, et al., Ann. Hematol. 70 259 (1995) , (3) V. Ricci, et al., BBRC 292, 167 (2002) , (4) Y. Ni, et al., Anal. Biochem. 352, 231 (2006) , (5) C.D. Link, et al., J. Biol. Chem. 281, 1808 (2006) , (6) G. Repetto, et al., Nat. Protoc. 3, 1125 (2008) , (7) M.A. Rauf, et al., Chem. Cent. J. 2, 19 (2008) , (8) B. Chazotte, Cold Spring Harb. Protoc. 2011, pdb.prot5570 (2011) , (9) G. Ates, et al., Methods Mol. Biol. 1601, 19 (2017) , (10) L. Xu, et al., Chem. Commun. 52, 14330 (2016)
InChi: InChI=1S/C15H16N4.ClH/c1-9-6-13-15(8-11(9)16)18-14-7-10(19(2)3)4-5-12(14)17-13 , /h4-8H,1-3H3,(H2,16,18) , 1H

Neutral red acts as a cellular pH indicator, changing from red to yellow between pH 6.8 and 8.0. More important, it is a dye used for staining in histology. It stains lysosomes red and is used as a general stain in histology, as a counterstain in combination with other dyes, and for many staining methods. It is applied in histology for nuclear staining, fluorescence staining, polychrome staining or for counter staining in Gram staining. It has also been incorporated in investigation of viruses, as a pH indicator, determination of DNA with optical and electrochemical methods, evalutaion of synthetic and naturally derived biomaterials. In microbiology, it is used in the MacConkey agar to differentiate bacteria for lactose fermentation. Neutral red is often used as a vital stain. Live cells incorporate neutral red into their lysosomes. As cells begin to die, their ability to incorporate neutral red diminishes. Thus, loss of neutral red uptake corresponds to loss of cell viability. The compound once taken up by cells can be used to acertain cell viability with a excitation maximum at 470nm and emission maximum at 580nm. Neutral red is added to some growth media for bacterial and cell cultures. Neutral red can be electrochemically polymerized and used as a redox mediator.

SKU: N0249 Category: Non Fluorescent Detection & Chromogene Detection

Additional information

Additional information

Synonyms	C.I. 50040, 3-Amino-7-dimethylamino-2-methylphenazine hydrochloride, Basic Red 5, Toluylene red
Purity	for microscopy
Appearance	Red to dark red powder
CAS-Number	553-24-2
Molecular Formula	C15H17CIN4
Molecular Weight	288.78
Identity	1H-NMR
Solvents	water (10 mg/ml)
Smiles	CC1=C(N)C=C(NC(C2=N3)=C/C(C=C2)=[N+](C)/C)C3=C1.[Cl-]
InChi Key	GIBXNAFNURIQII-UHFFFAOYSA-N
Shipping	AMBIENT
Short Term Storage	RT
Long Term Storage	RT
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at RT.
Transportation	Not dangerous goods
References	(1) J. Winckler, Red. Prog. Histochem. Cytochem. 6, 1 (1974), (2) P. Antal, et al., Ann. Hematol. 70 259 (1995), (3) V. Ricci, et al., BBRC 292, 167 (2002), (4) Y. Ni, et al., Anal. Biochem. 352, 231 (2006), (5) C.D. Link, et al., J. Biol. Chem. 281, 1808 (2006), (6) G. Repetto, et al., Nat. Protoc. 3, 1125 (2008), (7) M.A. Rauf, et al., Chem. Cent. J. 2, 19 (2008), (8) B. Chazotte, Cold Spring Harb. Protoc. 2011, pdb.prot5570 (2011), (9) G. Ates, et al., Methods Mol. Biol. 1601, 19 (2017), (10) L. Xu, et al., Chem. Commun. 52, 14330 (2016)
InChi	InChI=1S/C15H16N4.ClH/c1-9-6-13-15(8-11(9)16)18-14-7-10(19(2)3)4-5-12(14)17-13, /h4-8H,1-3H3,(H2,16,18), 1H
Quantity	25 g, 100 g, Bulk