Specifications
- Purity
- ≥98% (HPLC)
- Appearance
- Green to brown powder
- Identity
- 1H-NMR
Properties
- Solvents
- chloroform
Available from stock
Nile Red
- SKU
- N0107
Categories: Fluorescent Detection, Fluorescent Detection
- Synonyms
- Nile Blue A Oxazone , Phenoxazone 9 , 9-(Diethylamino)-5H-benzo[a]phenoxazin-5-one
- 7385-67-3
- CAS-Number
- C20H18N2O2
- Molecular Formula
- 318.4
- Molecular Weight
- Shipping
- AMBIENT
- Short Term Storage
- +4°C
- Long Term Storage
- -20°C
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at -20°C.
- Transportation
- Not dangerous goods
- Description
- Uncharged, hydrophobic, photostable fluorescent probe that strongly fluoresces bright red in hydrophobic (lipid-rich) environments, but is almost nonfluorescent in water. This lipophilic stain is commonly used for the detection of intracellular lipid droplets in cells such as adipocytes by fluorescence microscopy and flow cytometry. It has been shown to detect the exposure or formation of new hydrophobic surfaces induced by ligand binding to calmodulin, oligomerization of melittin, or unfolding of ovalbumin during early thermal denaturation. This product has been employed to stain cell structures, as well as to visualize and localize colloidal drug carriers. Has been used to stain lipophilic proteins that have been separated by SDS-PAGE electrophoresis or to detect sphingolipids on thin-layer chromatograms. The fluorescence is unaffected between pH 4.5 and 8.5 and may be used in dilute concentrations to investigate hydrophobic protein sites. Spectral properties: Intracellular fat vacuoles, filled with neutral lipids such as cholesterol, lipoproteins and triglycerides will fluoresce green (Abs/Em =485/525) while polar lipids, such as phospholipids, will fluoresce red (Abs/Em = 552/636 nm).
- Smiles
- CCN(CC)C1=CC=C2N=C3C(OC2=C1)=CC(=O)C1=C3C=CC=C1
- InChi Key
- VOFUROIFQGPCGE-UHFFFAOYSA-N
- References
- (1) P. Greenspan, et al.: J. Cell Biol. 100, 965 (1985) , (2) S.D. Fowler & P. Greenspan: J. Histochem. Cytochem. 33, 833 (1985) , (3) D.L. Sackett & J. Wolff: Anal. Biochem. 167, 228 (1987) , (4) F.J. Alba, et al: Biotechniques 21, 625 (1996) , (5) A. Lamprecht, et al.: J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 787, 415 (2003) , (6) A. Makino, et al.: Biochem. 45, 4530 (2006) , (7) T. Werner, et al.: Cytometry A 69, 200 (2006) , (8) B.W. Halstead, et al.: J. Appl. Toxicol. 26, 169 (2006) , (9) R. Varghese, et al.: Chemistry 16, 9040 (2010)
- InChi
- InChI=1S/C20H18N2O2/c1-3-22(4-2)13-9-10-16-18(11-13)24-19-12-17(23)14-7-5-6-8-15(14)20(19)21-16/h5-12H,3-4H2,1-2H3
Uncharged, hydrophobic, photostable fluorescent probe that strongly fluoresces bright red in hydrophobic (lipid-rich) environments, but is almost nonfluorescent in water. This lipophilic stain is commonly used for the detection of intracellular lipid droplets in cells such as adipocytes by fluorescence microscopy and flow cytometry. It has been shown to detect the exposure or formation of new hydrophobic surfaces induced by ligand binding to calmodulin, oligomerization of melittin, or unfolding of ovalbumin during early thermal denaturation. This product has been employed to stain cell structures, as well as to visualize and localize colloidal drug carriers. Has been used to stain lipophilic proteins that have been separated by SDS-PAGE electrophoresis or to detect sphingolipids on thin-layer chromatograms. The fluorescence is unaffected between pH 4.5 and 8.5 and may be used in dilute concentrations to investigate hydrophobic protein sites. Spectral properties: Intracellular fat vacuoles, filled with neutral lipids such as cholesterol, lipoproteins and triglycerides will fluoresce green (Abs/Em =485/525) while polar lipids, such as phospholipids, will fluoresce red (Abs/Em = 552/636 nm).
Additional information
| Synonyms | Nile Blue A Oxazone, Phenoxazone 9, 9-(Diethylamino)-5H-benzo[a]phenoxazin-5-one |
|---|---|
| Purity | ≥98% (HPLC) |
| Appearance | Green to brown powder |
| CAS-Number | 7385-67-3 |
| Molecular Formula | C20H18N2O2 |
| Molecular Weight | 318.4 |
| Identity | 1H-NMR |
| Solvents | chloroform |
| Smiles | CCN(CC)C1=CC=C2N=C3C(OC2=C1)=CC(=O)C1=C3C=CC=C1 |
| InChi Key | VOFUROIFQGPCGE-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Transportation | Not dangerous goods |
| References | (1) P. Greenspan, et al.: J. Cell Biol. 100, 965 (1985), (2) S.D. Fowler & P. Greenspan: J. Histochem. Cytochem. 33, 833 (1985), (3) D.L. Sackett & J. Wolff: Anal. Biochem. 167, 228 (1987), (4) F.J. Alba, et al: Biotechniques 21, 625 (1996), (5) A. Lamprecht, et al.: J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 787, 415 (2003), (6) A. Makino, et al.: Biochem. 45, 4530 (2006), (7) T. Werner, et al.: Cytometry A 69, 200 (2006), (8) B.W. Halstead, et al.: J. Appl. Toxicol. 26, 169 (2006), (9) R. Varghese, et al.: Chemistry 16, 9040 (2010) |
| InChi | InChI=1S/C20H18N2O2/c1-3-22(4-2)13-9-10-16-18(11-13)24-19-12-17(23)14-7-5-6-8-15(14)20(19)21-16/h5-12H,3-4H2,1-2H3 |
| Quantity | 1 g, 5 g, Bulk |
