Available from stock

N,N,N’,N’-Tetramethyl-p-phenylenediamine dihydrochloride

SKU: T0511

Category: Building Blocks, Intermediates, Reagents

Synonyms: TMPD, Tetramethyl-p-phenylenediamine dihydrochloride, Wurster's reagent dihydrochloride, 1,4-Bis(dimethylamino)benzene dihydrochloride; 637-01-4
CAS-Number: C₁₀H₁₈Cl₂N₂
Molecular Formula: 237.17
Molecular Weight

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥95% (NMR)
Appearance: Pale-yellow solid
Identity: 1H-NMR

Properties

Solvents: water (50 mg/ml)

Shipping: AMBIENT
Short Term Storage: RT
Long Term Storage: RT
Handling Advice: Protect from light and moisture.
Use / Stability: Stable for at least 2 years after receipt when stored at RT.

Hazard statements: H302+H312+H332-H315-H319
Precautionary statements: P261-P264-P270-P271-P280-P301+P312+P330-P302+P352+P312+P362+P364-P304+P340+P312-P305+P351+P338+P337+P313-P501
GHS Symbol: GHS07
Signal word: Warning
Transportation: Not dangerous goods

Description: N,N,N′,N′-Tetramethyl-p-phenylenediamine dihydrochloride (TMPD) is a redox-active aromatic amine compound, widely used in biochemical and analytical applications, particularly as a chromogenic redox indicator. TMPD is commonly used in redox titrations and biochemical assays. It is colorless in its reduced form and turns blue or purple (called Wurster’s Blue) upon oxidation, indicating electron transfer activity. This makes it valuable for detecting the presence or activity of oxidases or cytochromes. TMPD serves as an artificial electron donor, particularly to cytochrome c oxidase (complex IV) in the mitochondrial electron transport chain. It's often used in combination with ascorbate to assess respiratory chain function. TMPD is used in colorimetric assays for peroxidase activity or other oxidoreductases, where a color change signals enzyme action.
Smiles: N(C)(C)C1=CC=C(N(C)C)C=C1.Cl
InChi Key: FBHKTSXMTASXFJ-UHFFFAOYSA-N
References: (1) M.G. Mustafa & T.E. King, Arch. Biochem. Biophys. 122, 501 (1967) , (2) H.K. Kimelberg & P. Nicholls, Arch. Biochem. Biophys. 133, 327 (1969) , (3) S. Papa, et al., FEBS Lett. 157, 15 (1983) , (4) R. Munday, Chem. Biol. Interact. 65, 133 (1988) , (5) R.M. Lorence, et al., J. Biol. Chem. 263, 5271 (1988) , (6) E.W. de Vrind-de Jong, et al., Appl. Environ. Microbiol. 56, 3458 (1990) , (7) C. Storle & P. Eyer, Chem. Biol. Interact. 78, 321 (1991) , (8) N. Petrovic & M. Murray, Methods Mol. Biol. 594, 129 (2010)
InChi: InChI=1S/C10H16N2.2ClH/c1-11(2)9-5-7-10(8-6-9)12(3)4,,/h5-8H,1-4H3,2*1H

N,N,N′,N′-Tetramethyl-p-phenylenediamine dihydrochloride (TMPD) is a redox-active aromatic amine compound, widely used in biochemical and analytical applications, particularly as a chromogenic redox indicator. TMPD is commonly used in redox titrations and biochemical assays. It is colorless in its reduced form and turns blue or purple (called Wurster’s Blue) upon oxidation, indicating electron transfer activity. This makes it valuable for detecting the presence or activity of oxidases or cytochromes. TMPD serves as an artificial electron donor, particularly to cytochrome c oxidase (complex IV) in the mitochondrial electron transport chain. It’s often used in combination with ascorbate to assess respiratory chain function. TMPD is used in colorimetric assays for peroxidase activity or other oxidoreductases, where a color change signals enzyme action.

SKU: T0511 Category: Building Blocks, Intermediates, Reagents

Additional information

Additional information

Synonyms	TMPD, Tetramethyl-p-phenylenediamine dihydrochloride, Wurster's reagent dihydrochloride, 1,4-Bis(dimethylamino)benzene dihydrochloride
Purity	≥95% (NMR)
Appearance	Pale-yellow solid
CAS-Number	637-01-4
Molecular Formula	C10H18Cl2N2
Molecular Weight	237.17
Identity	1H-NMR
Solvents	water (50 mg/ml)
Smiles	N(C)(C)C1=CC=C(N(C)C)C=C1.Cl
InChi Key	FBHKTSXMTASXFJ-UHFFFAOYSA-N
Shipping	AMBIENT
Short Term Storage	RT
Long Term Storage	RT
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at RT.
Hazard statements	H302+H312+H332-H315-H319
Precautionary statements	P261-P264-P270-P271-P280-P301+P312+P330-P302+P352+P312+P362+P364-P304+P340+P312-P305+P351+P338+P337+P313-P501
GHS Symbol	GHS07
Signal word	Warning
Transportation	Not dangerous goods
References	(1) M.G. Mustafa & T.E. King, Arch. Biochem. Biophys. 122, 501 (1967), (2) H.K. Kimelberg & P. Nicholls, Arch. Biochem. Biophys. 133, 327 (1969), (3) S. Papa, et al., FEBS Lett. 157, 15 (1983), (4) R. Munday, Chem. Biol. Interact. 65, 133 (1988), (5) R.M. Lorence, et al., J. Biol. Chem. 263, 5271 (1988), (6) E.W. de Vrind-de Jong, et al., Appl. Environ. Microbiol. 56, 3458 (1990), (7) C. Storle & P. Eyer, Chem. Biol. Interact. 78, 321 (1991), (8) N. Petrovic & M. Murray, Methods Mol. Biol. 594, 129 (2010)
InChi	InChI=1S/C10H16N2.2ClH/c1-11(2)9-5-7-10(8-6-9)12(3)4,,/h5-8H,1-4H3,2*1H
Quantity	5 g, 25 g, Bulk