Available from stock

PBFI

SKU: P0662

Categories: Fluorescent Detection, Fluorescent Detection

Synonyms: Potassium-binding Benzofuran Isophthalate; 124549-11-7
CAS-Number: C₄₆H₄₆N₂O₁₆
Molecular Formula: 882.86
Molecular Weight

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Product Data
Handling
Safety Information
Details

Specifications

Purity: ~80% (HPLC)
Appearance: Brownish-yellow powder

Properties

Solvents: Soluble in methanol or DMSO.

Shipping: AMBIENT
Short Term Storage: -20°C
Long Term Storage: -20°C
Handling Advice: Protect from light and moisture.
Use / Stability: Stable for at least 2 years after receipt when stored at -20°C.

Transportation: Not dangerous goods

Description: PBFI is a cell-impermeant fluorescent indicator for potassium ions, suitable for detection of physiological concentrations of K+ in the presence of other monovalent cations. PBFI is used to measure intracellular potassium (K+) fluxes in animal cells and in plant cells and vacuoles. The observation that intracellular K+ levels are a controlling factor in apoptotic cell death pathways, increased the interest for PBFI. PBFI has been used for detecting adrenoceptor-stimulated decreases of intracellular K+ concentration in astrocytes and neurons, evaluating the mediating effects of K+ depletion on monocytic cell necrosis, measuring intracellular K+ fluxes associated with apoptotic cell shrinkage, monitoring mitochondrial KATP channel activation, or detecting elevated intracellular K+ levels associated with HIV-induced cytopathology. Flow cytometric measurements using UV argon-ion laser excitation (351nm and 364nm) of PBFI indicate that K+ efflux induces shrinkage of apoptotic cells and is a trigger for caspase activation. Spectral data: λex 336nm;, λem 557nm.
Smiles: COC1=CC2=C(OC(C3=C(C(O)=O)C=C(C(O)=O)C=C3)=C2)C=C1N4CCOCCOCCN(C5=CC(OC(C6=C(C(O)=O)C=C(C(O)=O)C=C6)=C7)=C7C=C5OC)CCOCCOCC4
InChi Key: YOQMJMHTHWYNIO-UHFFFAOYSA-N
References: (1) P. Jezek, et al.; J. Biol. Chem. 265, 10522 (1990) , (2) S.E. Kasner & M.B. Ganz; Am. J. Physiol. 262, F462 (1992) , (3) K. Venema, et al., Biochim. Biophys. Acta 1146, 87 (1993) , (4) R. Crossley, et al.; J. Chem. Soc. Perk. Trans. 2, 513 (1994) , (5) K. Meuwis; Biophys. J. 68, 2469 (1995) , (6) H. Muyderman, et al.; Neurochem. Int. 38, 269 (2001) , (7) A.L. Cook, et al.; Cell Signal. 14, 1023 (2002) , (8) S.J. Halperin & J.P. Lynch; J. Exp. Bot. 54, 2035 (2003) , (9) D. Liu, et al.; J. Neurochem. 86, 966 (2003) , (10) A.D. Costa, et al.; Am. J. Physiol. Heart Circ. Physiol. 290, H406 (2006) , (11) P. Andersson, et al.; Toxicol. In Vitro 20, 986 (2006) , (12) S. Jorgensen, et al.; Methods Mol. Biol. 491, 257 (2008) , (13) R.W. Sabnis; Handbook of biological dyes and stains (2010) , (14) C.S. Arlehamn, et al.; J. Biol. Chem. 285, 10508 (2010)
InChi: (1) J.W. Lee, et al.: Bull. Korean Chem. Soc. 26, 1790 (2005)

PBFI is a cell-impermeant fluorescent indicator for potassium ions, suitable for detection of physiological concentrations of K+ in the presence of other monovalent cations. PBFI is used to measure intracellular potassium (K+) fluxes in animal cells and in plant cells and vacuoles. The observation that intracellular K+ levels are a controlling factor in apoptotic cell death pathways, increased the interest for PBFI. PBFI has been used for detecting adrenoceptor-stimulated decreases of intracellular K+ concentration in astrocytes and neurons, evaluating the mediating effects of K+ depletion on monocytic cell necrosis, measuring intracellular K+ fluxes associated with apoptotic cell shrinkage, monitoring mitochondrial KATP channel activation, or detecting elevated intracellular K+ levels associated with HIV-induced cytopathology. Flow cytometric measurements using UV argon-ion laser excitation (351nm and 364nm) of PBFI indicate that K+ efflux induces shrinkage of apoptotic cells and is a trigger for caspase activation. Spectral data: λex 336nm;, λem 557nm.

SKU: P0662 Categories: Fluorescent Detection, Fluorescent Detection

Additional information

Additional information

Synonyms	Potassium-binding Benzofuran Isophthalate
Purity	~80% (HPLC)
Appearance	Brownish-yellow powder
CAS-Number	124549-11-7
Molecular Formula	C46H46N2O16
Molecular Weight	882.86
Solvents	Soluble in methanol or DMSO.
Smiles	COC1=CC2=C(OC(C3=C(C(O)=O)C=C(C(O)=O)C=C3)=C2)C=C1N4CCOCCOCCN(C5=CC(OC(C6=C(C(O)=O)C=C(C(O)=O)C=C6)=C7)=C7C=C5OC)CCOCCOCC4
InChi Key	YOQMJMHTHWYNIO-UHFFFAOYSA-N
Shipping	AMBIENT
Short Term Storage	-20°C
Long Term Storage	-20°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at -20°C.
Transportation	Not dangerous goods
References	(1) P. Jezek, et al.; J. Biol. Chem. 265, 10522 (1990), (2) S.E. Kasner & M.B. Ganz; Am. J. Physiol. 262, F462 (1992), (3) K. Venema, et al., Biochim. Biophys. Acta 1146, 87 (1993), (4) R. Crossley, et al.; J. Chem. Soc. Perk. Trans. 2, 513 (1994), (5) K. Meuwis; Biophys. J. 68, 2469 (1995), (6) H. Muyderman, et al.; Neurochem. Int. 38, 269 (2001), (7) A.L. Cook, et al.; Cell Signal. 14, 1023 (2002), (8) S.J. Halperin & J.P. Lynch; J. Exp. Bot. 54, 2035 (2003), (9) D. Liu, et al.; J. Neurochem. 86, 966 (2003), (10) A.D. Costa, et al.; Am. J. Physiol. Heart Circ. Physiol. 290, H406 (2006), (11) P. Andersson, et al.; Toxicol. In Vitro 20, 986 (2006), (12) S. Jorgensen, et al.; Methods Mol. Biol. 491, 257 (2008), (13) R.W. Sabnis; Handbook of biological dyes and stains (2010), (14) C.S. Arlehamn, et al.; J. Biol. Chem. 285, 10508 (2010)
InChi	(1) J.W. Lee, et al.: Bull. Korean Chem. Soc. 26, 1790 (2005)
Quantity	1 mg, Bulk