Specifications
- Purity
- ~80% (HPLC)
- Appearance
- Brownish-yellow powder
Properties
- Solvents
- Soluble in methanol or DMSO.
Category: Fluorescent Detection, Fluorescent Detection
PBFI is a cell-impermeant fluorescent indicator for potassium ions, suitable for detection of physiological concentrations of K+ in the presence of other monovalent cations. PBFI is used to measure intracellular potassium (K+) fluxes in animal cells and in plant cells and vacuoles. The observation that intracellular K+ levels are a controlling factor in apoptotic cell death pathways, increased the interest for PBFI. PBFI has been used for detecting adrenoceptor-stimulated decreases of intracellular K+ concentration in astrocytes and neurons, evaluating the mediating effects of K+ depletion on monocytic cell necrosis, measuring intracellular K+ fluxes associated with apoptotic cell shrinkage, monitoring mitochondrial KATP channel activation, or detecting elevated intracellular K+ levels associated with HIV-induced cytopathology. Flow cytometric measurements using UV argon-ion laser excitation (351nm and 364nm) of PBFI indicate that K+ efflux induces shrinkage of apoptotic cells and is a trigger for caspase activation. Spectral data: λex 336nm;, λem 557nm.
Synonyms | Potassium-binding Benzofuran Isophthalate |
---|---|
Purity | ~80% (HPLC) |
Appearance | Brownish-yellow powder |
CAS-Number | 124549-11-7 |
Molecular Formula | C46H46N2O16 |
Molecular Weight | 882.86 |
Solvents | Soluble in methanol or DMSO. |
Smiles | COC1=CC2=C(OC(C3=C(C(O)=O)C=C(C(O)=O)C=C3)=C2)C=C1N4CCOCCOCCN(C5=CC(OC(C6=C(C(O)=O)C=C(C(O)=O)C=C6)=C7)=C7C=C5OC)CCOCCOCC4 |
InChi Key | YOQMJMHTHWYNIO-UHFFFAOYSA-N |
Shipping | AMBIENT |
Short Term Storage | -20°C |
Long Term Storage | -20°C |
Handling Advice | Protect from light and moisture. |
Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
Transportation | Not dangerous goods |
References | (1) P. Jezek, et al.; J. Biol. Chem. 265, 10522 (1990), (2) S.E. Kasner & M.B. Ganz; Am. J. Physiol. 262, F462 (1992), (3) K. Venema, et al., Biochim. Biophys. Acta 1146, 87 (1993), (4) R. Crossley, et al.; J. Chem. Soc. Perk. Trans. 2, 513 (1994), (5) K. Meuwis; Biophys. J. 68, 2469 (1995), (6) H. Muyderman, et al.; Neurochem. Int. 38, 269 (2001), (7) A.L. Cook, et al.; Cell Signal. 14, 1023 (2002), (8) S.J. Halperin & J.P. Lynch; J. Exp. Bot. 54, 2035 (2003), (9) D. Liu, et al.; J. Neurochem. 86, 966 (2003), (10) A.D. Costa, et al.; Am. J. Physiol. Heart Circ. Physiol. 290, H406 (2006), (11) P. Andersson, et al.; Toxicol. In Vitro 20, 986 (2006), (12) S. Jorgensen, et al.; Methods Mol. Biol. 491, 257 (2008), (13) R.W. Sabnis; Handbook of biological dyes and stains (2010), (14) C.S. Arlehamn, et al.; J. Biol. Chem. 285, 10508 (2010) |
InChi | (1) J.W. Lee, et al.: Bull. Korean Chem. Soc. 26, 1790 (2005) |
Quantity | 1 mg, Bulk |