Specifications
- Purity
- ≥80% (Dye content)
- Appearance
- Green to dark green powder
Properties
- Solvents
- DMSO (20mg/ml)
- Melting Point
- ≥300 °C (lit.)
Documentation
- Safety Data Sheet (SDS)
- CDX P0525 MSDS.pdf
Available from stock
Phenosafranin
- SKU
- P0525
Category: Non Fluorescent Detection & Chromogene Detection
- Synonyms
- Phenosafranine, 3,7-Diamino-5-phenylphenazinium chloride, C.I. 50200, NSC215209, NSC 9855
- 81-93-6
- CAS-Number
- C18H15ClN4
- Molecular Formula
- 322.79
- Molecular Weight
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
- Shipping
- AMBIENT
- Short Term Storage
- RT
- Long Term Storage
- RT
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at RT.
- Hazard statements
- _x000D_H315 - H319 - H335
- Precautionary statements
- P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
- GHS Symbol
- GHS07
- Signal word
- Warning
- Transportation
- Not dangerous goods
- Description
- Phenosafranin is a phenazine dye which is an organic chloride salt having 3,7-diamino-5-phenylphenazin-5-ium as the counterion. It has a high binding affinity to triplex RNA compared to the parent duplex form, and binds through intercalation to both forms of RNA. This property makes it useful for staining plant cells and for the determination of hemoglobin, dopamine and serotonin. It binds also to double-stranded DNA. It has a role as a fluorochrome (e.g. to functionalize different reagents), a histological dye and a photosensitizing agent. Phenosafranin has also shown biological properties, such as inhibit nuclear localization of transglutaminase 2 without affecting its transamidase activity. Spectral Data: Absorbance ?max=519nm.
- Smiles
- NC1=CC=2[N+](=C3C(=NC2C=C1)C=CC(N)=C3)C4=CC=CC=C4.[Cl-]
- InChi Key
- SOUHUMACVWVDME-UHFFFAOYSA-N
- References
- (1) E.J. Moore, Science 77, 23 (1933) , (2) S. Brenner, Biochim. Biophys. Acta 11, 480 (1953) , (3) J.M. Widholm, Stain Technol. 47, 189 (1972) , (4) S.A. Curran, et al., J. Chem. Phys. 120, 4886 (2004) , (5) I. Saha, et al., J. Phys. Chem. B 114, 15278 (2010) , (6) I. Saha & G.S. Kumar, Fluoresc. 21, 247 (2011) , (7) W. Liu, et al., J. Sol. Chem. 40, 231 (2011) , (8) M.F. Broglia, et al., Photochem. Photobiol. Sci. 14, 407 (2015) , (9) B. Saha & G.S. Kumar, J. Photochem. Photobiol. B 161, 129 (2016) , (10) A.B. Pradhan, et al., Int. J. Biol. Macromol. 86, 345 (2016) , (11) Y. Furutani, et al., Amino Acids 49, 483 (2017) , (12) J. Hou, et al., Sensors 20, 1501 (2020) , (13) K. Rozga-Wijas, et al., Int. J. Mol. Sci. 22, 13373 (2021)
- InChi
- InChI=1S/C18H14N4.ClH/c19-12-6-8-15-17(10-12)22(14-4-2-1-3-5-14)18-11-13(20)7-9-16(18)21-15,/h1-11H,(H3,19,20),1H
Phenosafranin is a phenazine dye which is an organic chloride salt having 3,7-diamino-5-phenylphenazin-5-ium as the counterion. It has a high binding affinity to triplex RNA compared to the parent duplex form, and binds through intercalation to both forms of RNA. This property makes it useful for staining plant cells and for the determination of hemoglobin, dopamine and serotonin. It binds also to double-stranded DNA. It has a role as a fluorochrome (e.g. to functionalize different reagents), a histological dye and a photosensitizing agent. Phenosafranin has also shown biological properties, such as inhibit nuclear localization of transglutaminase 2 without affecting its transamidase activity. Spectral Data: Absorbance ?max=519nm.
Additional information
| Synonyms | Phenosafranine, 3,7-Diamino-5-phenylphenazinium chloride, C.I. 50200, NSC215209, NSC 9855 |
|---|---|
| Purity | ≥80% (Dye content) |
| Appearance | Green to dark green powder |
| CAS-Number | 81-93-6 |
| Molecular Formula | C18H15ClN4 |
| Molecular Weight | 322.79 |
| Solvents | DMSO (20mg/ml) |
| Melting Point | ≥300 °C (lit.) |
| Smiles | NC1=CC=2[N+](=C3C(=NC2C=C1)C=CC(N)=C3)C4=CC=CC=C4.[Cl-] |
| InChi Key | SOUHUMACVWVDME-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | RT |
| Long Term Storage | RT |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at RT. |
| Hazard statements | _x000D_H315 – H319 – H335 |
| Precautionary statements | P261 – P264 – P271 – P280 – P302 + P352 – P305 + P351 + P338 |
| GHS Symbol | GHS07 |
| Signal word | Warning |
| Transportation | Not dangerous goods |
| References | (1) E.J. Moore, Science 77, 23 (1933), (2) S. Brenner, Biochim. Biophys. Acta 11, 480 (1953), (3) J.M. Widholm, Stain Technol. 47, 189 (1972), (4) S.A. Curran, et al., J. Chem. Phys. 120, 4886 (2004), (5) I. Saha, et al., J. Phys. Chem. B 114, 15278 (2010), (6) I. Saha & G.S. Kumar, Fluoresc. 21, 247 (2011), (7) W. Liu, et al., J. Sol. Chem. 40, 231 (2011), (8) M.F. Broglia, et al., Photochem. Photobiol. Sci. 14, 407 (2015), (9) B. Saha & G.S. Kumar, J. Photochem. Photobiol. B 161, 129 (2016), (10) A.B. Pradhan, et al., Int. J. Biol. Macromol. 86, 345 (2016), (11) Y. Furutani, et al., Amino Acids 49, 483 (2017), (12) J. Hou, et al., Sensors 20, 1501 (2020), (13) K. Rozga-Wijas, et al., Int. J. Mol. Sci. 22, 13373 (2021) |
| InChi | InChI=1S/C18H14N4.ClH/c19-12-6-8-15-17(10-12)22(14-4-2-1-3-5-14)18-11-13(20)7-9-16(18)21-15,/h1-11H,(H3,19,20),1H |
| Quantity | 5 g, 25 g, Bulk |
