Propidium Iodide solution, 1.0 mg/ml in water

SKU: P0523

Category: Fluorescent Detection

Synonyms: 3,8-Diamino-5-(3-diethylaminopropyl)-6-phenylphenanthridinium iodide methiodide; 25535-16-4
CAS-Number: C₂₇H₃₄I₂N₄
Molecular Formula: 668.39
Molecular Weight

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥94% (HPLC)
Identity: 1H-NMR
Appearance: Slightly red liquid

Properties

Density: 1.0040 g/cm3
Solvents: water

Short Term Storage: +4°C
Use / Stability: Stable for at least 2 years after receipt when stored at -20°C.
Handling Advice: Protect from light and moisture.
Long Term Storage: -20°C
Shipping: AMBIENT

Transportation: Not dangerous goods

Description: Most common red-fluorescent nuclear stain. Membrane impermeant and generally excluded from viable cells. It can easily penetrate dead or damaged cells and as such is commonly used for identifying cell viability in a population or as a counterstain in multicolor fluorescent techniques. It binds to DNA and RNA by intercalating between the bases. Suitable for use in fluorescence microscopy, confocal laser scanning microscopy, flow cytometry and fluorometry. Spectral data: λex=488-535nm, λem=617nm.
InChi: InChI=1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20,,/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3,2*1H/q+1,,/p-1
References: (1) J. Fried et al., J. Cell Biol. 71(1), 172 (1976) , (2) (4) G. Ciancio et al., Cytometry 14(2), 205 (1993) , (3) D.M. Coder, Curr. Protoc. Cytom. Suppl. 15 9.2.1-9.2.14 (1997) , (4) C. Foglieni et al., Histochem. Cell Biol. 115(3), 223 (2001) , (5) T. Kovacs et al., Cytometry, Part A 73A(10), 965 (2008) , (6) M.K. Gould et al., Anal. Bioche. 382(2), 87 (2008)
InChi Key: XJMOSONTPMZWPB-UHFFFAOYSA-M
Smiles: C[N+](CC)(CCC[N+](C1=C2C=CC(N)=C1)=C(C3=C2C=CC(N)=C3)C4=CC=CC=C4)CC.[I-].[I-]

Most common red-fluorescent nuclear stain. Membrane impermeant and generally excluded from viable cells. It can easily penetrate dead or damaged cells and as such is commonly used for identifying cell viability in a population or as a counterstain in multicolor fluorescent techniques. It binds to DNA and RNA by intercalating between the bases. Suitable for use in fluorescence microscopy, confocal laser scanning microscopy, flow cytometry and fluorometry. Spectral data: λex=488-535nm, λem=617nm.

SKU: P0523 Category: Fluorescent Detection

Additional information

Additional information

Synonyms	3,8-Diamino-5-(3-diethylaminopropyl)-6-phenylphenanthridinium iodide methiodide
Purity	≥94% (HPLC)
Appearance	Slightly red liquid
CAS-Number	25535-16-4
Molecular Formula	C27H34I2N4
Molecular Weight	668.39
Identity	1H-NMR
Solvents	water
Density	1.0040 g/cm3
Smiles	C[N+](CC)(CCC[N+](C1=C2C=CC(N)=C1)=C(C3=C2C=CC(N)=C3)C4=CC=CC=C4)CC.[I-].[I-]
InChi Key	XJMOSONTPMZWPB-UHFFFAOYSA-M
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at -20°C.
Transportation	Not dangerous goods
References	(1) J. Fried et al., J. Cell Biol. 71(1), 172 (1976), (2) (4) G. Ciancio et al., Cytometry 14(2), 205 (1993), (3) D.M. Coder, Curr. Protoc. Cytom. Suppl. 15 9.2.1-9.2.14 (1997), (4) C. Foglieni et al., Histochem. Cell Biol. 115(3), 223 (2001), (5) T. Kovacs et al., Cytometry, Part A 73A(10), 965 (2008), (6) M.K. Gould et al., Anal. Bioche. 382(2), 87 (2008)
InChi	InChI=1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20,,/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3,2*1H/q+1,,/p-1
Quantity	25 ml, Bulk