Available from stock

Rhodamine 110 chloride

SKU: R0016

Categories: Fluorescent Detection, Fluorescent Detection

Synonyms: RH110 , Rhodamine 560 Chloride , Rhodamine N , 3,6-Diamino-9-(2-carboxyphenyl)xanthylium chloride; 13558-31-1
CAS-Number: C₂₀H₁₄N₂O₃ . HCl
Molecular Formula: 366.8
Molecular Weight

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥97% (HPLC)
Appearance: Red to red-brown crystalline or powder
Identity: 1H-NMR

Properties

Solvents: ethanol, methanol, DMSO, DMF, water (slightly)
Melting Point: >300 °C (lit.)
Refractive Index: n20D ~1.71 (Predicted)
Fluorescence: λex 498 nm , λem 520 nm in methanol

Documentation

Safety Data Sheet (SDS): CDX R0016 MSDS.pdf

Shipping: AMBIENT
Short Term Storage: RT
Long Term Storage: RT
Handling Advice: Protect from light and moisture.
Use / Stability: Stable for at least 2 years after receipt when stored at RT.

Transportation: Not dangerous goods

Description: Fluorescent dye. Fluorophore standard that is widely used in enzyme assay systems. Precursor for a variety of R110-based enzyme substrates for flow cytometric measurements. Used as a calibration standard for R110-based enzyme substrates. Compared to AMC and AFC enzyme substrates, fluorogenic R110 substrates are generally more sensitive, giving longer absorption and fluorescence wavelength than AMC and AFC substrates. Spectral data: ?ex 498nm| ?em 520nm in methanol.
Smiles: NC1=CC=C2C(OC(C3=C2C4=C(C(O)=O)C=CC=C4)=CC(C=C3)=N)=C1.Cl
InChi Key: JNGRENQDBKMCCR-UHFFFAOYSA-N
References: (1) R.F. Kubin, & A.N. Fletcher , J. Luminesc. 27, 455 (1983) , (2) E. Koller , Appl. Fluoresc. Technol. 3, 20 (1991) , (3) S. Klingel, et al.: Methods Cell Biol. 41, 449 (1994) (Review) , (4) S. Ganesh, et al.: Cytometry 20, 334 (1995) , (5) H. Hug, et al.: Biochemistry 38, 13906 (1999) , (6) X. Zhang, et al.: Spectrochim. Acta Part A: Mol. Biomol. Spectrosc. 59A, 1667 (2003) , (7) H. Abe, et al.: Bioconjugate Chem. 19, 1219 (2008) , (8) R.W. Watkins, et al.: Org. Biomol. Chem. 7, 3969 (2009)
InChi: InChI=1S/C20H14N2O3.ClH/c21-11-5-7-15-17(9-11)25-18-10-12(22)6-8-16(18)19(15)13-3-1-2-4-14(13)20(23)24

Fluorescent dye. Fluorophore standard that is widely used in enzyme assay systems. Precursor for a variety of R110-based enzyme substrates for flow cytometric measurements. Used as a calibration standard for R110-based enzyme substrates. Compared to AMC and AFC enzyme substrates, fluorogenic R110 substrates are generally more sensitive, giving longer absorption and fluorescence wavelength than AMC and AFC substrates. Spectral data: ?ex 498nm| ?em 520nm in methanol.

SKU: R0016 Categories: Fluorescent Detection, Fluorescent Detection

Additional information

Additional information

Synonyms	RH110, Rhodamine 560 Chloride, Rhodamine N, 3,6-Diamino-9-(2-carboxyphenyl)xanthylium chloride
Purity	≥97% (HPLC)
Appearance	Red to red-brown crystalline or powder
CAS-Number	13558-31-1
Molecular Formula	C20H14N2O3 . HCl
Molecular Weight	366.8
Identity	1H-NMR
Solvents	ethanol, methanol, DMSO, DMF, water (slightly)
Melting Point	>300 °C (lit.)
Refractive Index	n20D ~1.71 (Predicted)
Fluorescence	λex 498 nm, λem 520 nm in methanol
Smiles	NC1=CC=C2C(OC(C3=C2C4=C(C(O)=O)C=CC=C4)=CC(C=C3)=N)=C1.Cl
InChi Key	JNGRENQDBKMCCR-UHFFFAOYSA-N
Shipping	AMBIENT
Short Term Storage	RT
Long Term Storage	RT
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at RT.
Transportation	Not dangerous goods
References	(1) R.F. Kubin, & A.N. Fletcher, J. Luminesc. 27, 455 (1983), (2) E. Koller, Appl. Fluoresc. Technol. 3, 20 (1991), (3) S. Klingel, et al.: Methods Cell Biol. 41, 449 (1994) (Review), (4) S. Ganesh, et al.: Cytometry 20, 334 (1995), (5) H. Hug, et al.: Biochemistry 38, 13906 (1999), (6) X. Zhang, et al.: Spectrochim. Acta Part A: Mol. Biomol. Spectrosc. 59A, 1667 (2003), (7) H. Abe, et al.: Bioconjugate Chem. 19, 1219 (2008), (8) R.W. Watkins, et al.: Org. Biomol. Chem. 7, 3969 (2009)
InChi	InChI=1S/C20H14N2O3.ClH/c21-11-5-7-15-17(9-11)25-18-10-12(22)6-8-16(18)19(15)13-3-1-2-4-14(13)20(23)24
Quantity	250 mg, 1 g, Bulk