Specifications
- Purity
- ≥
- Appearance
- Violett crystalline solid
- Identity
- 1H-NMR
Properties
- Solvents
- DMSO (20mg/ml)
- Melting Point
- 182.0 to 186.0 °C
- UV / Vis
- 554 nm
- Fluorescence
- Exc 510 nm Em 578 nm
Available from stock
Rhodamine B hydrazide
- SKU
- R0178
Category: Fluorescent Detection
- Synonyms
- 2-Amino-3',6'-bis(diethylamino)-spiro[1H-isoindole-1,9'-[9H]xanthen]-3(2H)-one
- 74317-53-6
- CAS-Number
- C28H32N4O2
- Molecular Formula
- 456.59
- Molecular Weight
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
- Shipping
- AMBIENT
- Short Term Storage
- RT
- Long Term Storage
- -20°C
- Handling Advice
- Protect from light and moisture.
- Hazard statements
- H302-H315-H319-H335
- Precautionary statements
- P261-P264-P270-P271-P280-P301+P312-P302+P352-P304+P340-P305+P351+P338-P330-P332+P313-P337+P313-P362-P403+P233-P405-P501
- GHS Symbol
- GHS07
- Signal word
- Warning
- Transportation
- Not dangerous goods
- Description
- Rhodamine B hydrazide is a water-soluble fluorescent probe. Rhodamine B hydrazide can be used to label biomolecules (e.g., proteins, sugars) that have aldehyde or ketone groups. Spectral Data: λex 510nm, λem 578nm. It has been used for the detection of copper, peroxynitrite, nitric oxide, hydrogen peroxide, glucose, diacetyl, carbonyl, cholesterol and hemoglobin.
- Smiles
- CCN(CC)C1=CC2=C(C=C1)C3(C(C=CC=C4)=C4C(N3N)=O)C5=CC=C(N(CC)CC)C=C5O2
- InChi Key
- WTDHTIVYKKLOTC-UHFFFAOYSA-N
- References
- (1) V. Dujols, et al., JACS 119, 7386 (1997) , (2) X.F. Yang, et al., Talanta 57, 883 (2002) , (3) X.F. Yang, et al., Talanta 61, 439 (2003) , (4) X. Chen & Z. Jianli, Microchim. Acta 157, 133 (2007) , (5) Y. Shen, et al., J. Fluoresc. 18, 163 (2008) , (6) X. Li, et al., J. Fluoresc. 19, 601 (2009) , (7) C.M. Wu, et al., Anal. Chim. Acta 708, 141 (2011) , (8) P. Li, et al., Free Radic. Biol. Med. 65, 224 (2013) , (9) M. Asano, et al., J. Biosci. Bioeng. 118, 98 (2014) , (10) C.D. Georgiou, et al., Redox Biol. 17, 236 (2018) , (11) H. Zhang, et al., Anal. Chem. 97, 4583 (2025)
- InChi
- InChI=1S/C28H32N4O2/c1-5-30(6-2)19-13-15-23-25(17-19)34-26-18-20(31(7-3)8-4)14-16-24(26)28(23)22-12-10-9-11-21(22)27(33)32(28)29/h9-18H,5-8,29H2,1-4H3
Rhodamine B hydrazide is a water-soluble fluorescent probe. Rhodamine B hydrazide can be used to label biomolecules (e.g., proteins, sugars) that have aldehyde or ketone groups. Spectral Data: λex 510nm, λem 578nm. It has been used for the detection of copper, peroxynitrite, nitric oxide, hydrogen peroxide, glucose, diacetyl, carbonyl, cholesterol and hemoglobin.
Additional information
| Synonyms | 2-Amino-3',6'-bis(diethylamino)-spiro[1H-isoindole-1,9'-[9H]xanthen]-3(2H)-one |
|---|---|
| Purity | ≥ |
| Appearance | Violett crystalline solid |
| CAS-Number | 74317-53-6 |
| Molecular Formula | C28H32N4O2 |
| Molecular Weight | 456.59 |
| Identity | 1H-NMR |
| Solvents | DMSO (20mg/ml) |
| Melting Point | 182.0 to 186.0 °C |
| UV / Vis | 554 nm |
| Fluorescence | Exc 510 nm Em 578 nm |
| Smiles | CCN(CC)C1=CC2=C(C=C1)C3(C(C=CC=C4)=C4C(N3N)=O)C5=CC=C(N(CC)CC)C=C5O2 |
| InChi Key | WTDHTIVYKKLOTC-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | RT |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Hazard statements | H302-H315-H319-H335 |
| Precautionary statements | P261-P264-P270-P271-P280-P301+P312-P302+P352-P304+P340-P305+P351+P338-P330-P332+P313-P337+P313-P362-P403+P233-P405-P501 |
| GHS Symbol | GHS07 |
| Signal word | Warning |
| Transportation | Not dangerous goods |
| References | (1) V. Dujols, et al., JACS 119, 7386 (1997), (2) X.F. Yang, et al., Talanta 57, 883 (2002), (3) X.F. Yang, et al., Talanta 61, 439 (2003), (4) X. Chen & Z. Jianli, Microchim. Acta 157, 133 (2007), (5) Y. Shen, et al., J. Fluoresc. 18, 163 (2008), (6) X. Li, et al., J. Fluoresc. 19, 601 (2009), (7) C.M. Wu, et al., Anal. Chim. Acta 708, 141 (2011), (8) P. Li, et al., Free Radic. Biol. Med. 65, 224 (2013), (9) M. Asano, et al., J. Biosci. Bioeng. 118, 98 (2014), (10) C.D. Georgiou, et al., Redox Biol. 17, 236 (2018), (11) H. Zhang, et al., Anal. Chem. 97, 4583 (2025) |
| InChi | InChI=1S/C28H32N4O2/c1-5-30(6-2)19-13-15-23-25(17-19)34-26-18-20(31(7-3)8-4)14-16-24(26)28(23)22-12-10-9-11-21(22)27(33)32(28)29/h9-18H,5-8,29H2,1-4H3 |
| Quantity | 50 mg, 100 mg, Bulk |
