Available from stock

Stains-all

SKU: S0012

Categories: Fluorescent Detection, Fluorescent Detection

Synonyms: 1-Ethyl-2-[3-(1-ethylnaphtho[1,2-d]thiazolin-2-ylidene)-2-methylpropenyl]naphtho[1,2-d]thiazolium bromide, 3,3'-Diethyl-9-methyl-4,5,4',5'-dibenzothiacarbocyanine; 7423-31-6
CAS-Number: C₃₀H₂₇BrN₂S₂
Molecular Formula: 559.60
Molecular Weight

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥95% (NMR)
Appearance: Dark green powder
Identity: 1H-NMR

Properties

Solvents: Soluble in chloroform (10mg/ml), water or DMSO (10mg/ml).
UV / Vis: λmax 593-603 nm in chloroform

Shipping: AMBIENT
Short Term Storage: +4°C
Long Term Storage: +4°C
Handling Advice: Protect from light and moisture.
Use / Stability: Stable for at least 2 years after receipt when stored at -20°C.

Transportation: Not dangerous goods

Description: Stains-all is a carbocyanine dye, which stains anionic proteins, nucleic acids, anionic polysaccharides and other anionic molecules. Stains-all is metachromatic and changes its color dependent on its contact to other molecules. The detection limit for phosphoproteins is below 1ng after one hour of staining, for anionic polysaccharides between 10 and 500ng. Highly anionic proteins are stained blue, proteoglycans purple and anionic proteins pink. RNA is stained blueish-purple with a detection limit of 90ng and DNA is stained blue with a detection limit of 3ng. Stains-all is light sensitive, therefore the staining is performed in the absence of light and photographed immediately. Staining of proteins can be improved by a subsequent silver stain. Stains-all stains nucleic acids, anionic proteins, anionic polysaccharides such as alginate and pectinate, hyaluronic acid and dermatan sulfate, heparin, heparan sulfate and chondroitin sulfate. Stains-all is an ideal dye for the visualization and identification of proteins on polyacrylamide gels because it differentiates between highly acidic proteins which stain blue and less acidic proteins which stain pink. It is used in SDS-PAGE, agarose gel electrophoresis and histologic staining. A staining solution is typically made by dissolving the dye in formamide and buffer. Spectral data: λex 573nm; λem 609nm.
Smiles: CC[N+]1=C(/C=C(/C=C(S2)/N(CC)C3=C2C=CC4=C3C=CC=C4)C)SC5=C1C6=C(C=CC=C6)C=C5.[Br-]
InChi Key: MPBRYMWMMKKRGC-UHFFFAOYSA-M
References: (1) M.R. Green, et al.; Anal. Biochem. 56, 43 (1973) , (2) M.R. Green & J.V. Pastewka; J. Histochem. Cytochem. 27, 797 (1979) , (3) K.P. Campbell, et al.; J. Biol. Chem. 258, 11267 (1983) , (4) A.G. Krishna & Y. Sharma; Ind. J. Biochem. Biophys. 28, 30 (1991) , (5) Y. Sharma, et al.; FEBS Lett. 326, 59 (1993) , (6) J.M. Myers, et al.; Anal. Biochem. 240, 300 (1996) , (7) H.A. Goldberg & K.J. Warner; Anal. Biochem. 251, 227 (1997) , (8) N. Volpi & F. MacCari; Electrophoresis 23, 4060 (2002) , (9) N. Volpi, et al.; J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 820, 131 (2005) , (10) W.T. Cong, et al.; Electrophoresis 34, 3277 (2013) , (11) J.P.S. Andrade, et al.; Electrophoresis 39, 666 (2018) , (12) N. Sharma, et al.; Food Chem. 340, 127923 (2021)
InChi: (1) E. Delort et al.: J. Org. Chem. 71(12), 4468 (2006) , (2) E. Delort et al.: J. Am. Chem. Soc. 126(48), 15642 (2004) , (3) O. S. Wolfbeis et al.: Anal. Biochem. 129, 365 (1983)

Stains-all is a carbocyanine dye, which stains anionic proteins, nucleic acids, anionic polysaccharides and other anionic molecules. Stains-all is metachromatic and changes its color dependent on its contact to other molecules. The detection limit for phosphoproteins is below 1ng after one hour of staining, for anionic polysaccharides between 10 and 500ng. Highly anionic proteins are stained blue, proteoglycans purple and anionic proteins pink. RNA is stained blueish-purple with a detection limit of 90ng and DNA is stained blue with a detection limit of 3ng. Stains-all is light sensitive, therefore the staining is performed in the absence of light and photographed immediately. Staining of proteins can be improved by a subsequent silver stain. Stains-all stains nucleic acids, anionic proteins, anionic polysaccharides such as alginate and pectinate, hyaluronic acid and dermatan sulfate, heparin, heparan sulfate and chondroitin sulfate. Stains-all is an ideal dye for the visualization and identification of proteins on polyacrylamide gels because it differentiates between highly acidic proteins which stain blue and less acidic proteins which stain pink. It is used in SDS-PAGE, agarose gel electrophoresis and histologic staining. A staining solution is typically made by dissolving the dye in formamide and buffer. Spectral data: λex 573nm; λem 609nm.

SKU: S0012 Categories: Fluorescent Detection, Fluorescent Detection

Additional information

Additional information

Synonyms	1-Ethyl-2-[3-(1-ethylnaphtho[1,2-d]thiazolin-2-ylidene)-2-methylpropenyl]naphtho[1,2-d]thiazolium bromide, 3,3'-Diethyl-9-methyl-4,5,4',5'-dibenzothiacarbocyanine
Purity	≥95% (NMR)
Appearance	Dark green powder
CAS-Number	7423-31-6
Molecular Formula	C30H27BrN2S2
Molecular Weight	559.60
Identity	1H-NMR
Solvents	Soluble in chloroform (10mg/ml), water or DMSO (10mg/ml).
UV / Vis	λmax 593-603 nm in chloroform
Smiles	CC[N+]1=C(/C=C(/C=C(S2)/N(CC)C3=C2C=CC4=C3C=CC=C4)C)SC5=C1C6=C(C=CC=C6)C=C5.[Br-]
InChi Key	MPBRYMWMMKKRGC-UHFFFAOYSA-M
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	+4°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at -20°C.
Transportation	Not dangerous goods
References	(1) M.R. Green, et al.; Anal. Biochem. 56, 43 (1973), (2) M.R. Green & J.V. Pastewka; J. Histochem. Cytochem. 27, 797 (1979), (3) K.P. Campbell, et al.; J. Biol. Chem. 258, 11267 (1983), (4) A.G. Krishna & Y. Sharma; Ind. J. Biochem. Biophys. 28, 30 (1991), (5) Y. Sharma, et al.; FEBS Lett. 326, 59 (1993), (6) J.M. Myers, et al.; Anal. Biochem. 240, 300 (1996), (7) H.A. Goldberg & K.J. Warner; Anal. Biochem. 251, 227 (1997), (8) N. Volpi & F. MacCari; Electrophoresis 23, 4060 (2002), (9) N. Volpi, et al.; J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 820, 131 (2005), (10) W.T. Cong, et al.; Electrophoresis 34, 3277 (2013), (11) J.P.S. Andrade, et al.; Electrophoresis 39, 666 (2018), (12) N. Sharma, et al.; Food Chem. 340, 127923 (2021)
InChi	(1) E. Delort et al.: J. Org. Chem. 71(12), 4468 (2006), (2) E. Delort et al.: J. Am. Chem. Soc. 126(48), 15642 (2004), (3) O. S. Wolfbeis et al.: Anal. Biochem. 129, 365 (1983)
Quantity	1 g, 5 g, Bulk