Specifications
- Purity
- ≥90% (NMR)
- Appearance
- White to off-white powder
- Identity
- 1H-NMR
Properties
- Solvents
- water (10 mg/ml), DMSO, DMF
Documentation
- Safety Data Sheet (SDS)
- CDX B0310 MSDS.pdf
Available from stock
Sulfo-NHS-LC-Biotin
- SKU
- B0310
Categories:
- Synonyms
- EZ-Link Sulfo-NHS-LC-Biotin , Sulfosuccinimidyl 6-(biotinamido)hexanoate , Biotinamidohexanoic acid 3-sulfo-N-hydroxysuccinimide ester sodium salt
- 191671-46-2
- CAS-Number
- C20H29N4NaO9S2
- Molecular Formula
- 556.59
- Molecular Weight
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
- Shipping
- AMBIENT
- Short Term Storage
- +4°C
- Long Term Storage
- -20°C
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at -20°C.
- Hazard statements
- H360D
- Precautionary statements
- P201-P308 + P313
- GHS Symbol
- GHS08
- Transportation
- Not dangerous goods
- Description
- Sulfo-NHS-LC-Biotin is an amino-reactive biotinylation reagent with a long spacer arm. The 6-aminocaproic acid spacer arm provides distance between the biotin and the target ligand which helps alleviate steric hindrance, in addition it forms permanent amide bonds. The sodium sulfonate groups render this compound water-soluble and prevent it from crossing the lipid bi-layer of the cell membrane, making it a tool to label cell surface proteins. In addition, Sulfo-NHS-LC-Biotin is used to biotinylate antibodies, proteins, peptides, or amino acids by reacting with primary amines as found in the N-terminus and Lysine side chains. Optimal reaction conditions are at a pH of 7-9. Avoid amine-containing buffers, which may compete in the acylation reaction.
- InChi Key
- JJGWLCLUQNFDIS-GTSONSFRSA-M
- References
- (1) G.T. Hermanson, Bioconjugate Techn. 2nd p506-545 (2008)
Sulfo-NHS-LC-Biotin is an amino-reactive biotinylation reagent with a long spacer arm. The 6-aminocaproic acid spacer arm provides distance between the biotin and the target ligand which helps alleviate steric hindrance, in addition it forms permanent amide bonds. The sodium sulfonate groups render this compound water-soluble and prevent it from crossing the lipid bi-layer of the cell membrane, making it a tool to label cell surface proteins. In addition, Sulfo-NHS-LC-Biotin is used to biotinylate antibodies, proteins, peptides, or amino acids by reacting with primary amines as found in the N-terminus and Lysine side chains. Optimal reaction conditions are at a pH of 7-9. Avoid amine-containing buffers, which may compete in the acylation reaction.
Additional information
| Synonyms | EZ-Link Sulfo-NHS-LC-Biotin, Sulfosuccinimidyl 6-(biotinamido)hexanoate, Biotinamidohexanoic acid 3-sulfo-N-hydroxysuccinimide ester sodium salt |
|---|---|
| Purity | ≥90% (NMR) |
| Appearance | White to off-white powder |
| CAS-Number | 191671-46-2 |
| Molecular Formula | C20H29N4NaO9S2 |
| Molecular Weight | 556.59 |
| Identity | 1H-NMR |
| Solvents | water (10 mg/ml), DMSO, DMF |
| InChi Key | JJGWLCLUQNFDIS-GTSONSFRSA-M |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Hazard statements | H360D |
| Precautionary statements | P201-P308 + P313 |
| GHS Symbol | GHS08 |
| Transportation | Not dangerous goods |
| References | (1) G.T. Hermanson, Bioconjugate Techn. 2nd p506-545 (2008) |
| Quantity | 10 mg, 50 mg, Bulk |
