Specifications
- Purity
- ≥98% (NMR)
- Appearance
- White to off-white powder
- Identity
- 1H-NMR
Properties
- Solvents
- water (50 mg/ml), DMSO (3 mg/ml)
- Melting Point
- 174-186 °C
Category: Building Blocks, Intermediates, Reagents
TCEP is an odorless, selective, and water-soluble strong reducing agent that is commonly used in biochemistry and molecular biology applications. It is commonly used to rapidly reduce protein and peptide disulfide bonds a preparatory step for gel electrophoresis. TCEP can be combined with proteases to simultaneously reduce and digest proteins prior to mass spectrometry in order to dramatically increase sequence coverage. More stable than DTT and useful in mass spectrometry applications. It has also been used to measure ascorbic acid and dehydroascorbic acid in biological samples. TCEP is a useful chelating agent for various heavy metal ions as Zn(II), Cd(II), Pb(II), and Ni(II).
Synonyms | Tris(2-carboxyethyl)phosphine hydrochloride |
---|---|
Purity | ≥98% (NMR) |
Appearance | White to off-white powder |
CAS-Number | 51805-45-9 |
Molecular Formula | C9H15O6P·HCl |
Molecular Weight | 286.60 |
Identity | 1H-NMR |
Solvents | water (50 mg/ml), DMSO (3 mg/ml) |
Melting Point | 174-186 °C |
Smiles | OC(CCP(CCC(O)=O)CCC(O)=O)=O.Cl |
InChi Key | PBVAJRFEEOIAGW-UHFFFAOYSA-N |
Shipping | AMBIENT |
Short Term Storage | +4°C |
Long Term Storage | +4°C |
Handling Advice | Protect from light and moisture. |
Use / Stability | Stable for at least 2 years after receipt when stored at +4°C. |
Hazard statements | H314 |
Precautionary statements | P280-P305 + P351 + P338-P310 |
GHS Symbol | GHS05 |
Signal word | Danger |
RIDADR | UN3261 |
Transportation | Packing Group II |
References | (1) J. Lykkesfeldt, Anal. Biochem. 282, 89 (2000), (2) C.C. Visser, et al., J. Drug Target 12, 569 (2004), (3) C. David, et al., Phys. Chem. Chem. Phys. 11, 2532 (2009), (4) P. Liu, et al., J. Am. Soc. Mass Spectrom. 21, 837 (2010), (5) H.M. Zhang, et al., Anal. Chem. 82, 1450 (2010) |
InChi | InChI=1S/C9H15O6P.ClH/c10-7(11)1-4-16(5-2-8(12)13)6-3-9(14)15,/h1-6H2,(H,10,11)(H,12,13)(H,14,15),1H |
Quantity | 2 g, 5 g, Bulk |