TCEP

SKU: T0176

Category: Building Blocks, Intermediates, Reagents

Synonyms: Tris(2-carboxyethyl)phosphine hydrochloride; 51805-45-9
CAS-Number: C₉H₁₅O₆P·HCl
Molecular Formula: 286.60
Molecular Weight

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥98% (NMR)
Identity: 1H-NMR
Appearance: White to off-white powder

Properties

Melting Point: 174-186 °C
Solvents: water (50 mg/ml), DMSO (3 mg/ml)

Handling Advice: Protect from light and moisture.
Use / Stability: Stable for at least 2 years after receipt when stored at +4°C.
Long Term Storage: +4°C
Short Term Storage: +4°C
Shipping: AMBIENT

Transportation: Packing Group II
RIDADR: UN3261
Signal word: Danger
GHS Symbol: GHS05
Precautionary statements: P280-P305 + P351 + P338-P310
Hazard statements: H314

Description: TCEP is an odorless, selective, and water-soluble strong reducing agent that is commonly used in biochemistry and molecular biology applications. It is commonly used to rapidly reduce protein and peptide disulfide bonds a preparatory step for gel electrophoresis. TCEP can be combined with proteases to simultaneously reduce and digest proteins prior to mass spectrometry in order to dramatically increase sequence coverage. More stable than DTT and useful in mass spectrometry applications. It has also been used to measure ascorbic acid and dehydroascorbic acid in biological samples. TCEP is a useful chelating agent for various heavy metal ions as Zn(II), Cd(II), Pb(II), and Ni(II).
InChi: InChI=1S/C9H15O6P.ClH/c10-7(11)1-4-16(5-2-8(12)13)6-3-9(14)15,/h1-6H2,(H,10,11)(H,12,13)(H,14,15),1H
References: (1) J. Lykkesfeldt, Anal. Biochem. 282, 89 (2000) , (2) C.C. Visser, et al., J. Drug Target 12, 569 (2004) , (3) C. David, et al., Phys. Chem. Chem. Phys. 11, 2532 (2009) , (4) P. Liu, et al., J. Am. Soc. Mass Spectrom. 21, 837 (2010) , (5) H.M. Zhang, et al., Anal. Chem. 82, 1450 (2010)
InChi Key: PBVAJRFEEOIAGW-UHFFFAOYSA-N
Smiles: OC(CCP(CCC(O)=O)CCC(O)=O)=O.Cl

TCEP is an odorless, selective, and water-soluble strong reducing agent that is commonly used in biochemistry and molecular biology applications. It is commonly used to rapidly reduce protein and peptide disulfide bonds a preparatory step for gel electrophoresis. TCEP can be combined with proteases to simultaneously reduce and digest proteins prior to mass spectrometry in order to dramatically increase sequence coverage. More stable than DTT and useful in mass spectrometry applications. It has also been used to measure ascorbic acid and dehydroascorbic acid in biological samples. TCEP is a useful chelating agent for various heavy metal ions as Zn(II), Cd(II), Pb(II), and Ni(II).

SKU: T0176 Category: Building Blocks, Intermediates, Reagents

Additional information

Additional information

Synonyms	Tris(2-carboxyethyl)phosphine hydrochloride
Purity	≥98% (NMR)
Appearance	White to off-white powder
CAS-Number	51805-45-9
Molecular Formula	C9H15O6P·HCl
Molecular Weight	286.60
Identity	1H-NMR
Solvents	water (50 mg/ml), DMSO (3 mg/ml)
Melting Point	174-186 °C
Smiles	OC(CCP(CCC(O)=O)CCC(O)=O)=O.Cl
InChi Key	PBVAJRFEEOIAGW-UHFFFAOYSA-N
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	+4°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at +4°C.
Hazard statements	H314
Precautionary statements	P280-P305 + P351 + P338-P310
GHS Symbol	GHS05
Signal word	Danger
RIDADR	UN3261
Transportation	Packing Group II
References	(1) J. Lykkesfeldt, Anal. Biochem. 282, 89 (2000), (2) C.C. Visser, et al., J. Drug Target 12, 569 (2004), (3) C. David, et al., Phys. Chem. Chem. Phys. 11, 2532 (2009), (4) P. Liu, et al., J. Am. Soc. Mass Spectrom. 21, 837 (2010), (5) H.M. Zhang, et al., Anal. Chem. 82, 1450 (2010)
InChi	InChI=1S/C9H15O6P.ClH/c10-7(11)1-4-16(5-2-8(12)13)6-3-9(14)15,/h1-6H2,(H,10,11)(H,12,13)(H,14,15),1H
Quantity	2 g, 5 g, Bulk