Specifications
- Purity
- ≥98% (HPLC)
- Appearance
- Yellow powder
- Identity
- 1H-NMR
Properties
- Solvents
- water (5 mg/ml)
Documentation
- Safety Data Sheet (SDS)
- CDX T0186 MSDS.pdf
Available from stock
Thiazolyl Blue Tetrazolium Bromide
- SKU
- T0186
Categories: Fluorescent Detection, Fluorescent Detection
- Synonyms
- 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide , MTT , Methylthiazolyldiphenyl-tetrazolium bromide , NSC 60102
- 298-93-1
- CAS-Number
- C18H16BrN5S
- Molecular Formula
- 414.32
- Molecular Weight
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
- Shipping
- AMBIENT
- Short Term Storage
- +4°C
- Long Term Storage
- +4°C
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at +4°C.
- Hazard statements
- H315-H319-H335-H341
- Precautionary statements
- P261-P281-P305 + P351 + P338
- GHS Symbol
- GHS07+GHS08
- Signal word
- Warning
- Transportation
- Not dangerous goods
- Description
- MTT is a common histochemical/cytochemical reagent and for the detection of NAD. MTT is used to detect reductive metabolism in cells. It is a cell-permeable yellow dye that is reduced by intracellular NAD(P)H-oxidoreductases in mitochondria of living cells to form the dark blue water-insoluble MTT-formazan. The intensity of the color can then be measured spectrophotometrically at 540 or 570nm, which allows quantitation of cell viability and cell proliferation. It is frequently used in colorimetric assays to measure cell proliferation/cell growth, cytotoxicity (such as for screening cancer drugs), and apoptosis.
- Smiles
- CC1=C(C)SC([N+]2=NC(C3=CC=CC=C3)=NN2C4=CC=CC=C4)=N1.[Br-]
- InChi Key
- AZKSAVLVSZKNRD-UHFFFAOYSA-M
- References
- (1) M.A. Abdallah, & J.-F. Biellmann, Eur. J. Biochem. 112, 331 (1980) , (2) Y. Liu, et al., J. Neurochem. 69, 581 (1997) , (3) M.V. Berridge, et al., Biotechnol. Ann. Rev. 11, 127 (2005) , (4) T. Hayon, et al., Leuk. Lymphoma 44, 1957 (2003) (Review) , (5) K. Praebst, et al., Methods Mol. Biol. 1601, 1 (2017) , (6) P. Kumar, et al., Cold Spring Harb. Protoc. 2018, pdb.prot095505 (2018) , (7) E. Grela, et al., Acta Histochem. 120, 303 (2018) (Review)
- InChi
- InChI=1S/C18H16N5S.BrH/c1-13-14(2)24-18(19-13)23-21-17(15-9-5-3-6-10-15)20-22(23)16-11-7-4-8-12-16 , /h3-12H,1-2H3 , 1H/q+1 , /p-1
MTT is a common histochemical/cytochemical reagent and for the detection of NAD. MTT is used to detect reductive metabolism in cells. It is a cell-permeable yellow dye that is reduced by intracellular NAD(P)H-oxidoreductases in mitochondria of living cells to form the dark blue water-insoluble MTT-formazan. The intensity of the color can then be measured spectrophotometrically at 540 or 570nm, which allows quantitation of cell viability and cell proliferation. It is frequently used in colorimetric assays to measure cell proliferation/cell growth, cytotoxicity (such as for screening cancer drugs), and apoptosis.
Additional information
| Synonyms | 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, MTT, Methylthiazolyldiphenyl-tetrazolium bromide, NSC 60102 |
|---|---|
| Purity | ≥98% (HPLC) |
| Appearance | Yellow powder |
| CAS-Number | 298-93-1 |
| Molecular Formula | C18H16BrN5S |
| Molecular Weight | 414.32 |
| Identity | 1H-NMR |
| Solvents | water (5 mg/ml) |
| Smiles | CC1=C(C)SC([N+]2=NC(C3=CC=CC=C3)=NN2C4=CC=CC=C4)=N1.[Br-] |
| InChi Key | AZKSAVLVSZKNRD-UHFFFAOYSA-M |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | +4°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at +4°C. |
| Hazard statements | H315-H319-H335-H341 |
| Precautionary statements | P261-P281-P305 + P351 + P338 |
| GHS Symbol | GHS07+GHS08 |
| Signal word | Warning |
| Transportation | Not dangerous goods |
| References | (1) M.A. Abdallah, & J.-F. Biellmann, Eur. J. Biochem. 112, 331 (1980), (2) Y. Liu, et al., J. Neurochem. 69, 581 (1997), (3) M.V. Berridge, et al., Biotechnol. Ann. Rev. 11, 127 (2005), (4) T. Hayon, et al., Leuk. Lymphoma 44, 1957 (2003) (Review), (5) K. Praebst, et al., Methods Mol. Biol. 1601, 1 (2017), (6) P. Kumar, et al., Cold Spring Harb. Protoc. 2018, pdb.prot095505 (2018), (7) E. Grela, et al., Acta Histochem. 120, 303 (2018) (Review) |
| InChi | InChI=1S/C18H16N5S.BrH/c1-13-14(2)24-18(19-13)23-21-17(15-9-5-3-6-10-15)20-22(23)16-11-7-4-8-12-16, /h3-12H,1-2H3, 1H/q+1, /p-1 |
| Quantity | 1 g, 5 g, 10 g, Bulk |
