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    TMB dihydrochloride

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      TMB dihydrochloride

      SKU
      T0151

      Category: Non Fluorescent Detection & Chromogene Detection

      Synonyms
      3,3',5,5'-Tetramethylbenzidine dihydrochloride , TMBZ
      64285-73-0
      CAS-Number
      C16H20N2 . 2HCl
      Molecular Formula
      313.27
      Molecular Weight

      • Product Data
      • Handling
      • Safety Information
      • Details

      Specifications

      Purity
      ≥98% (Titration)
      Identity
      1H-NMR
      Appearance
      White to off-white to pink powder

      Properties

      Melting Point
      ≥300 °C
      Solvents
      DMSO

      Downloads

      Safety Data Sheet
      CDX T0151 MSDS.pdf
      Short Term Storage
      +4°C
      Use / Stability
      Stable for at least 2 years after receipt when stored at +4°C.
      Handling Advice
      Protect from light and moisture.
      Long Term Storage
      +4°C
      Shipping
      AMBIENT
      Transportation
      Not dangerous goods
      Description
      TMBZ is a chromogenic reagent utilized for peroxidase detection. It has been developed as an alternative to benzidine. Although the TMBZ solution is colorless, it turns bluish-green (?max: 655 nm) in the presence of hydrogen peroxide and peroxidase. The structure of this bluish-green complex is thought to be a radical form of two oxidized TMBZ molecules. This peroxidase substrate is used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA). The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2M H2SO4 (resulting in a yellow color), and read at 450nm.
      InChi
      InChI=1S/C16H20N2.ClH/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14
      References
      (1) V.R. Holland, et al.: Tetrahedron 30, 3299 (1974) , (2) P.E. Thomas, et al.: Anal. Biochem. 75, 168 (1976) , (3) H.H. Liem, et al.: Anal. Biochem. 98, 388 (1979) , (4) E.S. Bos, et al.: J. Immunoassay 2, 187 (1981) , (5) K. Suzuki, et al.: Anal. Biochem. 132, 345 (1983) , (6) F.H. Pujol, et al.: J. Immunoassay. 14, 21 (1993) , (7) F.B. Serrat: Talanta 41, 2091 (1994)
      InChi Key
      OBWSOTREAMFOCQ-UHFFFAOYSA-N
      Smiles
      CC1=C(N)C(C)=CC(C2=CC(C)=C(N)C(C)=C2)=C1.Cl

      TMBZ is a chromogenic reagent utilized for peroxidase detection. It has been developed as an alternative to benzidine. Although the TMBZ solution is colorless, it turns bluish-green (?max: 655 nm) in the presence of hydrogen peroxide and peroxidase. The structure of this bluish-green complex is thought to be a radical form of two oxidized TMBZ molecules. This peroxidase substrate is used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA). The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2M H2SO4 (resulting in a yellow color), and read at 450nm.

      SKU: T0151 Category: Non Fluorescent Detection & Chromogene Detection
      • Additional information

      Additional information

      Synonyms

      3,3',5,5'-Tetramethylbenzidine dihydrochloride, TMBZ

      Purity

      ≥98% (Titration)

      Appearance

      White to off-white to pink powder

      CAS-Number

      64285-73-0

      Molecular Formula

      C16H20N2 . 2HCl

      Molecular Weight

      313.27

      Identity

      1H-NMR

      Solvents

      DMSO

      Melting Point

      ≥300 °C

      Smiles

      CC1=C(N)C(C)=CC(C2=CC(C)=C(N)C(C)=C2)=C1.Cl

      InChi Key

      OBWSOTREAMFOCQ-UHFFFAOYSA-N

      Shipping

      AMBIENT

      Short Term Storage

      +4°C

      Long Term Storage

      +4°C

      Handling Advice

      Protect from light and moisture.

      Use / Stability

      Stable for at least 2 years after receipt when stored at +4°C.

      Transportation

      Not dangerous goods

      References

      (1) V.R. Holland, et al.: Tetrahedron 30, 3299 (1974), (2) P.E. Thomas, et al.: Anal. Biochem. 75, 168 (1976), (3) H.H. Liem, et al.: Anal. Biochem. 98, 388 (1979), (4) E.S. Bos, et al.: J. Immunoassay 2, 187 (1981), (5) K. Suzuki, et al.: Anal. Biochem. 132, 345 (1983), (6) F.H. Pujol, et al.: J. Immunoassay. 14, 21 (1993), (7) F.B. Serrat: Talanta 41, 2091 (1994)

      InChi

      InChI=1S/C16H20N2.ClH/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14

      Quantity

      1 g, 5 g, Bulk

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