Specifications
- Purity
- ≥98% (Titration)
- Appearance
- White to off-white to pink powder
- Identity
- 1H-NMR
Properties
- Solvents
- DMSO
- Melting Point
- ≥300 °C
Documentation
- Safety Data Sheet (SDS)
- CDX T0151 MSDS.pdf
Available from stock
TMB dihydrochloride
- SKU
- T0151
Category: Non Fluorescent Detection & Chromogene Detection
- Synonyms
- 3,3',5,5'-Tetramethylbenzidine dihydrochloride , TMBZ
- 64285-73-0
- CAS-Number
- C16H20N2 . 2HCl
- Molecular Formula
- 313.27
- Molecular Weight
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
- Shipping
- AMBIENT
- Short Term Storage
- +4°C
- Long Term Storage
- +4°C
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at +4°C.
- Transportation
- Not dangerous goods
- Description
- TMBZ is a chromogenic reagent utilized for peroxidase detection. It has been developed as an alternative to benzidine. Although the TMBZ solution is colorless, it turns bluish-green (?max: 655 nm) in the presence of hydrogen peroxide and peroxidase. The structure of this bluish-green complex is thought to be a radical form of two oxidized TMBZ molecules. This peroxidase substrate is used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA). The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2M H2SO4 (resulting in a yellow color), and read at 450nm.
- Smiles
- CC1=C(N)C(C)=CC(C2=CC(C)=C(N)C(C)=C2)=C1.Cl
- InChi Key
- OBWSOTREAMFOCQ-UHFFFAOYSA-N
- References
- (1) V.R. Holland, et al.: Tetrahedron 30, 3299 (1974) , (2) P.E. Thomas, et al.: Anal. Biochem. 75, 168 (1976) , (3) H.H. Liem, et al.: Anal. Biochem. 98, 388 (1979) , (4) E.S. Bos, et al.: J. Immunoassay 2, 187 (1981) , (5) K. Suzuki, et al.: Anal. Biochem. 132, 345 (1983) , (6) F.H. Pujol, et al.: J. Immunoassay. 14, 21 (1993) , (7) F.B. Serrat: Talanta 41, 2091 (1994)
- InChi
- InChI=1S/C16H20N2.ClH/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14
TMBZ is a chromogenic reagent utilized for peroxidase detection. It has been developed as an alternative to benzidine. Although the TMBZ solution is colorless, it turns bluish-green (?max: 655 nm) in the presence of hydrogen peroxide and peroxidase. The structure of this bluish-green complex is thought to be a radical form of two oxidized TMBZ molecules. This peroxidase substrate is used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA). The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2M H2SO4 (resulting in a yellow color), and read at 450nm.
Additional information
| Synonyms | 3,3',5,5'-Tetramethylbenzidine dihydrochloride, TMBZ |
|---|---|
| Purity | ≥98% (Titration) |
| Appearance | White to off-white to pink powder |
| CAS-Number | 64285-73-0 |
| Molecular Formula | C16H20N2 . 2HCl |
| Molecular Weight | 313.27 |
| Identity | 1H-NMR |
| Solvents | DMSO |
| Melting Point | ≥300 °C |
| Smiles | CC1=C(N)C(C)=CC(C2=CC(C)=C(N)C(C)=C2)=C1.Cl |
| InChi Key | OBWSOTREAMFOCQ-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | +4°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at +4°C. |
| Transportation | Not dangerous goods |
| References | (1) V.R. Holland, et al.: Tetrahedron 30, 3299 (1974), (2) P.E. Thomas, et al.: Anal. Biochem. 75, 168 (1976), (3) H.H. Liem, et al.: Anal. Biochem. 98, 388 (1979), (4) E.S. Bos, et al.: J. Immunoassay 2, 187 (1981), (5) K. Suzuki, et al.: Anal. Biochem. 132, 345 (1983), (6) F.H. Pujol, et al.: J. Immunoassay. 14, 21 (1993), (7) F.B. Serrat: Talanta 41, 2091 (1994) |
| InChi | InChI=1S/C16H20N2.ClH/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14 |
| Quantity | 1 g, 5 g, Bulk |
