TMB dihydrochloride

SKU: T0151

Category: Non Fluorescent Detection & Chromogene Detection

Synonyms: 3,3',5,5'-Tetramethylbenzidine dihydrochloride , TMBZ; 64285-73-0
CAS-Number: C₁₆H₂₀N₂ . ₂HCl
Molecular Formula: 313.27
Molecular Weight

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥98% (Titration)
Identity: 1H-NMR
Appearance: White to off-white to pink powder

Properties

Melting Point: ≥300 °C
Solvents: DMSO

Downloads

Safety Data Sheet: CDX T0151 MSDS.pdf

Short Term Storage: +4°C
Use / Stability: Stable for at least 2 years after receipt when stored at +4°C.
Handling Advice: Protect from light and moisture.
Long Term Storage: +4°C
Shipping: AMBIENT

Transportation: Not dangerous goods

Description: TMBZ is a chromogenic reagent utilized for peroxidase detection. It has been developed as an alternative to benzidine. Although the TMBZ solution is colorless, it turns bluish-green (?max: 655 nm) in the presence of hydrogen peroxide and peroxidase. The structure of this bluish-green complex is thought to be a radical form of two oxidized TMBZ molecules. This peroxidase substrate is used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA). The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2M H2SO4 (resulting in a yellow color), and read at 450nm.
InChi: InChI=1S/C16H20N2.ClH/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14
References: (1) V.R. Holland, et al.: Tetrahedron 30, 3299 (1974) , (2) P.E. Thomas, et al.: Anal. Biochem. 75, 168 (1976) , (3) H.H. Liem, et al.: Anal. Biochem. 98, 388 (1979) , (4) E.S. Bos, et al.: J. Immunoassay 2, 187 (1981) , (5) K. Suzuki, et al.: Anal. Biochem. 132, 345 (1983) , (6) F.H. Pujol, et al.: J. Immunoassay. 14, 21 (1993) , (7) F.B. Serrat: Talanta 41, 2091 (1994)
InChi Key: OBWSOTREAMFOCQ-UHFFFAOYSA-N
Smiles: CC1=C(N)C(C)=CC(C2=CC(C)=C(N)C(C)=C2)=C1.Cl

TMBZ is a chromogenic reagent utilized for peroxidase detection. It has been developed as an alternative to benzidine. Although the TMBZ solution is colorless, it turns bluish-green (?max: 655 nm) in the presence of hydrogen peroxide and peroxidase. The structure of this bluish-green complex is thought to be a radical form of two oxidized TMBZ molecules. This peroxidase substrate is used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA). The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2M H2SO4 (resulting in a yellow color), and read at 450nm.

SKU: T0151 Category: Non Fluorescent Detection & Chromogene Detection

Additional information

Additional information

Synonyms	3,3',5,5'-Tetramethylbenzidine dihydrochloride, TMBZ
Purity	≥98% (Titration)
Appearance	White to off-white to pink powder
CAS-Number	64285-73-0
Molecular Formula	C16H20N2 . 2HCl
Molecular Weight	313.27
Identity	1H-NMR
Solvents	DMSO
Melting Point	≥300 °C
Smiles	CC1=C(N)C(C)=CC(C2=CC(C)=C(N)C(C)=C2)=C1.Cl
InChi Key	OBWSOTREAMFOCQ-UHFFFAOYSA-N
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	+4°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at +4°C.
Transportation	Not dangerous goods
References	(1) V.R. Holland, et al.: Tetrahedron 30, 3299 (1974), (2) P.E. Thomas, et al.: Anal. Biochem. 75, 168 (1976), (3) H.H. Liem, et al.: Anal. Biochem. 98, 388 (1979), (4) E.S. Bos, et al.: J. Immunoassay 2, 187 (1981), (5) K. Suzuki, et al.: Anal. Biochem. 132, 345 (1983), (6) F.H. Pujol, et al.: J. Immunoassay. 14, 21 (1993), (7) F.B. Serrat: Talanta 41, 2091 (1994)
InChi	InChI=1S/C16H20N2.ClH/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14
Quantity	1 g, 5 g, Bulk