Tris(hydroxymethyl)aminomethane
- SKU
- T0244
Category: Buffers
- Synonyms
- 2-Amino-2-(hydroxymethyl)-1,3-propanediol , THAM , Tris base , Trometamol
- 77-86-1
- CAS-Number
- C4H11NO3
- Molecular Formula
- 121.14
- Molecular Weight
Specifications
- Purity
- ≥99%
- Appearance
- White to off-white powder
- Identity
- 1H-NMR
Properties
- Solvents
- water (550 mg/ml), methanol (26 mg/ml), anhydrous ethanol (14.6 mg/ml), DMF (14 mg/ml), acetone (2 mg/ml)
- Melting Point
- 168-172 °C
Downloads
- Safety Data Sheet
- CDX T0244 MSDS.pdf
- Shipping
- AMBIENT
- Short Term Storage
- RT
- Long Term Storage
- RT
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at RT.
- Transportation
- Not dangerous goods
- Description
- A high quality buffer used in the range of physiological pH 7-9. Widely used as a biological buffer or as a component of buffer formulations. Tris has a pKa of 8.06 and is very useful in the biology and biochemistry lab because it has buffering capabilities in the range of the typical physiological pH of most living organisms (pH 7.0-9.0). Tris has been reported to interfere with the activity of a number of enzymes, it should therefore be used carefully when studying proteins.
- Smiles
- NC(CO)(CO)CO
- InChi Key
- LENZDBCJOHFCAS-UHFFFAOYSA-N
- References
- (1) R.H. Kallet, et al. , Am. J. Respir. Crit. Care Med. 161, 1149 (2000) , (2) W.T. Desmarais, et al. , Structure 10, 1063 (2002) , (3) Z. Ghalanbor, et al. , Protein Pept. Lett. 15, 212 (2008) , (4) J. Sambrook, & D.W. Russell , Molecular Cloning: A Laboratory Manual (2012)
A high quality buffer used in the range of physiological pH 7-9. Widely used as a biological buffer or as a component of buffer formulations. Tris has a pKa of 8.06 and is very useful in the biology and biochemistry lab because it has buffering capabilities in the range of the typical physiological pH of most living organisms (pH 7.0-9.0). Tris has been reported to interfere with the activity of a number of enzymes, it should therefore be used carefully when studying proteins.