Zincon monosodium salt

SKU: Z0028

Category: Non Fluorescent Detection & Chromogene Detection

Synonyms: 2-[5-(2-Hydroxy-5-sulfophenyl)-3-phenyl-1-formazyl]benzoic acid monosodium salt , 2-Carboxy-2'-hydroxy-5'-sulfoformazyl-benzene monosodium salt; 62625-22-3
CAS-Number: C₂₀H₁₅N₄NaO₆S
Molecular Formula: 462.41
Molecular Weight

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥85%
Identity: 1H-NMR
Appearance: Dark violet powder
Fluorescence: λmax 485-495 nm

Properties

Solvents: water (slightly at 20° C), alcohol (slightly), aqueous alkali

Downloads

Safety Data Sheet: CDX Z0028 MSDS.pdf

Use / Stability: Stable for at least 2 years after receipt when stored at RT.
Handling Advice: Protect from light and moisture.
Long Term Storage: RT
Short Term Storage: RT
Shipping: AMBIENT

Transportation: Not dangerous goods
Signal word: Warning
GHS Symbol: GHS07
Precautionary statements: P305 + P351 + P338
Hazard statements: H315-H319 + H335

Description: A colorimetric reagent for Zn, Cu and Hg detection and a metal indicator in EDTA titration. The aqueous solution of Zincon is yellow or orange-yellow in weak alkaline conditions, and turns blue in the presence of Cu (maximum wavelength, 600 nm, molar absorptivity, 1.9x104) and Zn (pH 8.5-9.5, maximum wavelength, 620 nm, molar absorptivity, 23,000). Used for the spectrophotometric determination of zinc, simultaneous determination of zinc and copper and for automated flow injection spectrometric determination of zinc. Binds nonspecifically proteins at acidic pH and was therefore used to stain proteins fast and background-free in sodium dodecyl sulfate polyacrylamide gel (SDS-Page). Sligthly less sensitivity than Coomassie Brilliant Blue®CBB but stains and destains more rapidly. Additionally, zincon staining is reversible, suggesting it might be applicable to 2-D PAGE and downstream analyses by mass spectrometry. Shown to inhibit insulin binding to its receptor and to attenuate the activity of ATPase and other enzymes, presumably because of the chelating properties of the dye.
InChi: InChI=1S/C20H16N4O6S.Na/c25-18-11-10-14(31(28,29)30)12-17(18)22-24-19(13-6-2-1-3-7-13)23-21-16-9-5-4-8-15(16)20(26)27,/h1-12,21,25H,(H,26,27)(H,28,29,30),/q,+1/p-1/b23-19+,24-22+,
References: G.K. Singhal & K.N. Tandon, Talanta 14, 1351 (1967) , K. Vytras, et al., Talanta 22, 529 (1975) , F. Mollinedo, et al., Eur J Biochem. 119, 183 (1981) , H. Ono & A. Ito, J. Biochem. 95, 345 (1984) , M.A. Koupparis, et al., The Analyst 111, 1311 (1986) , C.M. Corns, Ann. Clin. Biochem. 24, 591 (1987) , J.I. Monreal, et al., Rev. Esp. Fisiol. 43, 133 (1987) , E. Hilario, et al., J. Biochem. Biophys. Methods 21, 197 (1990) , R.M. Liu, et al., Talanta 40, 511 (1993) , J.K. Choi, et al., Electrophoresis 23, 4053 (2002) , G.B. Smejkal & H.F. Hoff, Biotechn. 34, 486 (2003) , C.E. Saebel, et al., Anal. Biochem. 397, 218 (2010)
InChi Key: DVHNWVSFUULGKM-SWJPHGMYSA-M
Smiles: O=S(C1=CC=C(O)C(/N=N/C(C2=CC=CC=C2)=N/NC3=CC=CC=C3C(O)=O)=C1)([O-])=O.[Na+]

A colorimetric reagent for Zn, Cu and Hg detection and a metal indicator in EDTA titration. The aqueous solution of Zincon is yellow or orange-yellow in weak alkaline conditions, and turns blue in the presence of Cu (maximum wavelength, 600 nm, molar absorptivity, 1.9×104) and Zn (pH 8.5-9.5, maximum wavelength, 620 nm, molar absorptivity, 23,000). Used for the spectrophotometric determination of zinc, simultaneous determination of zinc and copper and for automated flow injection spectrometric determination of zinc. Binds nonspecifically proteins at acidic pH and was therefore used to stain proteins fast and background-free in sodium dodecyl sulfate polyacrylamide gel (SDS-Page). Sligthly less sensitivity than Coomassie Brilliant Blue®CBB but stains and destains more rapidly. Additionally, zincon staining is reversible, suggesting it might be applicable to 2-D PAGE and downstream analyses by mass spectrometry. Shown to inhibit insulin binding to its receptor and to attenuate the activity of ATPase and other enzymes, presumably because of the chelating properties of the dye.

SKU: Z0028 Category: Non Fluorescent Detection & Chromogene Detection

Additional information

Additional information

Synonyms	2-[5-(2-Hydroxy-5-sulfophenyl)-3-phenyl-1-formazyl]benzoic acid monosodium salt, 2-Carboxy-2'-hydroxy-5'-sulfoformazyl-benzene monosodium salt
Purity	≥85%
Appearance	Dark violet powder
CAS-Number	62625-22-3
Molecular Formula	C20H15N4NaO6S
Molecular Weight	462.41
Identity	1H-NMR
Solvents	water (slightly at 20° C), alcohol (slightly), aqueous alkali
Smiles	O=S(C1=CC=C(O)C(/N=N/C(C2=CC=CC=C2)=N/NC3=CC=CC=C3C(O)=O)=C1)([O-])=O.[Na+]
InChi Key	DVHNWVSFUULGKM-SWJPHGMYSA-M
Shipping	AMBIENT
Short Term Storage	RT
Long Term Storage	RT
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at RT.
Hazard statements	H315-H319 + H335
Precautionary statements	P305 + P351 + P338
GHS Symbol	GHS07
Signal word	Warning
Transportation	Not dangerous goods
References	G.K. Singhal & K.N. Tandon, Talanta 14, 1351 (1967), K. Vytras, et al., Talanta 22, 529 (1975), F. Mollinedo, et al., Eur J Biochem. 119, 183 (1981), H. Ono & A. Ito, J. Biochem. 95, 345 (1984), M.A. Koupparis, et al., The Analyst 111, 1311 (1986), C.M. Corns, Ann. Clin. Biochem. 24, 591 (1987), J.I. Monreal, et al., Rev. Esp. Fisiol. 43, 133 (1987), E. Hilario, et al., J. Biochem. Biophys. Methods 21, 197 (1990), R.M. Liu, et al., Talanta 40, 511 (1993), J.K. Choi, et al., Electrophoresis 23, 4053 (2002), G.B. Smejkal & H.F. Hoff, Biotechn. 34, 486 (2003), C.E. Saebel, et al., Anal. Biochem. 397, 218 (2010)
InChi	InChI=1S/C20H16N4O6S.Na/c25-18-11-10-14(31(28,29)30)12-17(18)22-24-19(13-6-2-1-3-7-13)23-21-16-9-5-4-8-15(16)20(26)27,/h1-12,21,25H,(H,26,27)(H,28,29,30),/q,+1/p-1/b23-19+,24-22+,
Quantity	1 g, 5 g, Bulk
cdx-Fluorescence	λmax 485-495 nm