Specifications
- Purity
- ≥95% (HPCE)
- Identity
- 1H-NMR
- Appearance
- Solid
Properties
- Fluorescence
- λex 492 nm, λem 514 nm in PBS, Zn2+, esterase
- Solvents
- DMSO
Downloads
- Safety Data Sheet
- CDX Z0011 MSDS.pdf
Available from stock
ZnAF-1F DA
- SKU
- Z0011
Categories: Fluorescent Detection, Fluorescent Detection
- Synonyms
- 5-({2-[Bis(2-pyridinylmethyl)amino]ethyl}amino)-2-(2,7-difluoro-6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid diacetate
- n/a
- CAS-Number
- C38H30F2N4O7
- Molecular Formula
- 692.66
- Molecular Weight
- Short Term Storage
- +4°C
- Use / Stability
- Stable for at least 2 years after receipt when stored at -20°C.
- Handling Advice
- Protect from light and moisture.
- Long Term Storage
- -20°C
- Shipping
- AMBIENT
- Transportation
- Not dangerous goods
- Description
- For application to living cells, the diacetyl derivative of ZnAF-1F, ZnAF-1F DA, was synthesized. ZnAF-1F DA can permeate through the cell membrane, and is hydrolyzed by esterase in the cytosol to yield ZnAF-1F, which is retained in the cells. ZnAF-1F DA has been used to measure the changes of intracellular Zn2+ in cultured cells and hippocampal slices.
- InChi
- InChI=1S/C38H30F2N4O7/c1-22(45)48-35-18-33-29(16-31(35)39)38(30-17-32(40)36(49-23(2)46)19-34(30)50-33)28-10-9-24(15-27(28)37(47)51-38)43-13-14-44(20-25-7-3-5-11-41-25)21-26-8-4-6-12-42-26/h3-12,15-19,43H,13-14,20-21H2,1-2H3
- References
- (1) S. Ueno, et al.: J. Cell Biol. 158, 215 (2002) , (2) T. Hirano, et al.: JACS 124, 6555 (2002)
- InChi Key
- LPSDYSDKFVFVIH-UHFFFAOYSA-N
- Smiles
- CC(=O)OC1=CC2=C(C=C1F)C1(OC(=O)C3=C1C=CC(NCCN(CC1=CC=CC=N1)CC1=NC=CC=C1)=C3)C1=CC(F)=C(OC(C)=O)C=C1O2
For application to living cells, the diacetyl derivative of ZnAF-1F, ZnAF-1F DA, was synthesized. ZnAF-1F DA can permeate through the cell membrane, and is hydrolyzed by esterase in the cytosol to yield ZnAF-1F, which is retained in the cells. ZnAF-1F DA has been used to measure the changes of intracellular Zn2+ in cultured cells and hippocampal slices.
Additional information
| Synonyms | 5-({2-[Bis(2-pyridinylmethyl)amino]ethyl}amino)-2-(2,7-difluoro-6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid diacetate |
|---|---|
| Purity | ≥95% (HPCE) |
| Appearance | Solid |
| CAS-Number | n/a |
| Molecular Formula | C38H30F2N4O7 |
| Molecular Weight | 692.66 |
| Identity | 1H-NMR |
| Solvents | DMSO |
| Fluorescence | λex 492 nm, λem 514 nm in PBS, Zn2+, esterase |
| Smiles | CC(=O)OC1=CC2=C(C=C1F)C1(OC(=O)C3=C1C=CC(NCCN(CC1=CC=CC=N1)CC1=NC=CC=C1)=C3)C1=CC(F)=C(OC(C)=O)C=C1O2 |
| InChi Key | LPSDYSDKFVFVIH-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Transportation | Not dangerous goods |
| References | (1) S. Ueno, et al.: J. Cell Biol. 158, 215 (2002), (2) T. Hirano, et al.: JACS 124, 6555 (2002) |
| InChi | InChI=1S/C38H30F2N4O7/c1-22(45)48-35-18-33-29(16-31(35)39)38(30-17-32(40)36(49-23(2)46)19-34(30)50-33)28-10-9-24(15-27(28)37(47)51-38)43-13-14-44(20-25-7-3-5-11-41-25)21-26-8-4-6-12-42-26/h3-12,15-19,43H,13-14,20-21H2,1-2H3 |
| Quantity | 1 mg, 5 mg, Bulk |
