Specifications
- Purity
- ≥95% (HPCE)
- Appearance
- Liquid
- Identity
- 1H-NMR
Properties
- Solvents
- DMSO
- Fluorescence
- λex 492 nm, λem 515 nm in PBS, Zn2+
Available from stock
ZnAF-2 solution (5 mM in DMSO), 1 mg in 0.35 ml DMSO
- SKU
- Z0506
Categories: Fluorescent Detection, Fluorescent Detection
- Synonyms
- 6-{2-[Bis(2-pyridylmethyl)amino]ethylamino}fluorescein
- 321859-11-4
- CAS-Number
- C34H28N4O5
- Molecular Formula
- 572.61
- Molecular Weight
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
- Shipping
- AMBIENT
- Short Term Storage
- +4°C
- Long Term Storage
- -20°C
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at -20°C.
- Transportation
- Not dangerous goods
- Description
- A membrane-impermeable ethylenediaminofluoresceinyl compound that acts as a high-affinity Zn2+-specific fluorescent probe (Kd = 2.7 nM). It shows low basal fluorescence and the fluorescence intensity increases ~51-fold upon stoichiometric (1:1) binding to Zn2+. Exhibits little affinity towards Ca2+, Mg2+, Na+, or K+.
- Smiles
- OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=C(NCCN(CC1=CC=CC=N1)CC1=NC=CC=C1)C=C3)C1=CC=C(O)C=C1O2
- InChi Key
- IJNFGJFEJQCIIT-UHFFFAOYSA-N
- References
- (1) T. Hirano et al., J. Am. Chem. Soc. 124, 6555 (2002) , (2) T. Hirano et al., J. Am. Chem. Soc. 122, 12399 (2000)
- InChi
- InChI=1S/C34H28N4O5/c39-25-8-11-28-31(18-25)42-32-19-26(40)9-12-29(32)34(28)30-17-22(7-10-27(30)33(41)43-34)37-15-16-38(20-23-5-1-3-13-35-23)21-24-6-2-4-14-36-24/h1-14,17-19,37,39-40H,15-16,20-21H2
A membrane-impermeable ethylenediaminofluoresceinyl compound that acts as a high-affinity Zn2+-specific fluorescent probe (Kd = 2.7 nM). It shows low basal fluorescence and the fluorescence intensity increases ~51-fold upon stoichiometric (1:1) binding to Zn2+. Exhibits little affinity towards Ca2+, Mg2+, Na+, or K+.
Additional information
| Synonyms | 6-{2-[Bis(2-pyridylmethyl)amino]ethylamino}fluorescein |
|---|---|
| Purity | ≥95% (HPCE) |
| Appearance | Liquid |
| CAS-Number | 321859-11-4 |
| Molecular Formula | C34H28N4O5 |
| Molecular Weight | 572.61 |
| Identity | 1H-NMR |
| Solvents | DMSO |
| Fluorescence | λex 492 nm, λem 515 nm in PBS, Zn2+ |
| Smiles | OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=C(NCCN(CC1=CC=CC=N1)CC1=NC=CC=C1)C=C3)C1=CC=C(O)C=C1O2 |
| InChi Key | IJNFGJFEJQCIIT-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Transportation | Not dangerous goods |
| References | (1) T. Hirano et al., J. Am. Chem. Soc. 124, 6555 (2002), (2) T. Hirano et al., J. Am. Chem. Soc. 122, 12399 (2000) |
| InChi | InChI=1S/C34H28N4O5/c39-25-8-11-28-31(18-25)42-32-19-26(40)9-12-29(32)34(28)30-17-22(7-10-27(30)33(41)43-34)37-15-16-38(20-23-5-1-3-13-35-23)21-24-6-2-4-14-36-24/h1-14,17-19,37,39-40H,15-16,20-21H2 |
| Quantity | 1 mg, Bulk |
