Specifications
- Purity
- ≥97% (HPLC)
- Appearance
- White to off-white crystalline powder
- Identity
- 1H-NMR
Properties
- Solvents
- DMSO, DMF, EtOAc, CHCl3
Category: Fluorescent Detection, Fluorescent Detection
5,5?-Difluoro-BAPTA-AM is a calcium chelator that may be used to modulate calcium levels and flow within cells to study calcium homeostasis and cell signaling. It is used to study cytosolic free Ca2+ by 19F-NMR. It has high selectivity for Ca2+, it is not particularly pH sensitive and is available protected as its acetoxymethyl ester. Determination with 5,5?-Difluoro-BAPTA-AM are not sensitive to cells which have a high natural background fluorescence.
Synonyms | 5FBAPTA-AM, 1,2-Bis(2-amino-5-fluorophenoxy)ethane-N,N,N',N'-tetraacetic acid tetrakis(acetoxymethyl) ester |
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Purity | ≥97% (HPLC) |
Appearance | White to off-white crystalline powder |
CAS-Number | 128255-42-5 |
Molecular Formula | C34H38F2N2O18 |
Molecular Weight | 800.66 |
Identity | 1H-NMR |
Solvents | DMSO, DMF, EtOAc, CHCl3 |
Smiles | O=C(OCOC(C)=O)CN(CC(OCOC(C)=O)=O)C1=CC=C(F)C=C1OCCOC2=CC(F)=CC=C2N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O |
InChi Key | ZXXWTKAFGAXGHZ-UHFFFAOYSA-N |
Shipping | AMBIENT |
Short Term Storage | -20°C |
Long Term Storage | -20°C |
Handling Advice | Protect from light and moisture. |
Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
Hazard statements | H315-H319-H335 |
Precautionary statements | P261-P305+P351+P338 |
GHS Symbol | GHS07 |
Signal word | Warning |
Transportation | Not dangerous goods |
References | (1) G.A. Smith, et al., PNAS 80, 7178 (1983), (2) E. Marban, et al., Circul. Res. 66, 1255 (1990), (3) P.E. Morris, Org. Prep. Proc. Int. 25, 445 (1993), (4) A.R. Waldeck, et al., Eur. Biophys. J. 27, 247 (1998) |
InChi | InChI=1S/C34H38F2N2O18/c1-21(39)49-17-53-31(43)13-37(14-32(44)54-18-50-22(2)40)27-7-5-25(35)11-29(27)47-9-10-48-30-12-26(36)6-8-28(30)38(15-33(45)55-19-51-23(3)41)16-34(46)56-20-52-24(4)42/h5-8,11-12H,9-10,13-20H2,1-4H3 |
Quantity | 10 mg, 50 mg, Bulk |