5-TAMRA

SKU: C0055

Category: Fluorescent Detection

Synonyms: 5-Carboxytetramethylrhodamine; 91809-66-4
CAS-Number: C₂₅H₂₂N₂O₅
Molecular Formula: 430.45
Molecular Weight

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥97% (HPLC)
Identity: 1H-NMR
Appearance: Dark red powder

Properties

Fluorescence: λex 543 nm, λem 575 nm in 0.1 M Tris pH 8.0
Melting Point: 300 °C
Solvents: methanol, DMSO, DMF

Shipping: AMBIENT
Use / Stability: Stable for at least 2 years after receipt when stored at +4°C.
Handling Advice: Protect from light and moisture.
Long Term Storage: +4°C
Short Term Storage: +4°C

Transportation: Not dangerous goods

Description: Free acid form of 5-carboxytetramethylrhodamine single isomer. Widely used fluorophore for preparing bioconjugates, especially fluorescent antibody and avidin derivatives used in immunochemistry. Used as a dye for oligonucleotide labeling and automated DNA sequencing applications.
InChi: InChI=1S/C25H22N2O5/c1-26(2)15-6-9-18-21(12-15)32-22-13-16(27(3)4)7-10-19(22)23(18)17-8-5-14(24(28)29)11-20(17)25(30)31/h5-13H,1-4H3,(H-,28,29,30,31)
References: (1) A. Aneja et al.: J. Biol. Phys. 34(5), 487 (2008) , (2) C. Dose et al.: Bioorg. Med. Chem. 16(1), 65 (2008) , (3) G. Vamosi et al.: Fluorescence characteristics. Biophys. J. 71, 972 (1996) , (4) L.G. Lee et al.: Nucleic Acids Res. 20, 2471 (1992)
InChi Key: YMZMTOFQCVHHFB-UHFFFAOYSA-N
Smiles: CN(C)C1=CC=C2C(OC3=CC(C=CC3=C2C2=CC=C(C=C2C([O-])=O)C(O)=O)=[N+](C)C)=C1

Free acid form of 5-carboxytetramethylrhodamine single isomer. Widely used fluorophore for preparing bioconjugates, especially fluorescent antibody and avidin derivatives used in immunochemistry. Used as a dye for oligonucleotide labeling and automated DNA sequencing applications.

SKU: C0055 Category: Fluorescent Detection

Additional information

Additional information

Synonyms	5-Carboxytetramethylrhodamine
Purity	≥97% (HPLC)
Appearance	Dark red powder
CAS-Number	91809-66-4
Molecular Formula	C25H22N2O5
Molecular Weight	430.45
Identity	1H-NMR
Solvents	methanol, DMSO, DMF
Melting Point	300 °C
Fluorescence	λex 543 nm, λem 575 nm in 0.1 M Tris pH 8.0
Smiles	CN(C)C1=CC=C2C(OC3=CC(C=CC3=C2C2=CC=C(C=C2C([O-])=O)C(O)=O)=[N+](C)C)=C1
InChi Key	YMZMTOFQCVHHFB-UHFFFAOYSA-N
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	+4°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at +4°C.
Transportation	Not dangerous goods
References	(1) A. Aneja et al.: J. Biol. Phys. 34(5), 487 (2008), (2) C. Dose et al.: Bioorg. Med. Chem. 16(1), 65 (2008), (3) G. Vamosi et al.: Fluorescence characteristics. Biophys. J. 71, 972 (1996), (4) L.G. Lee et al.: Nucleic Acids Res. 20, 2471 (1992)
InChi	InChI=1S/C25H22N2O5/c1-26(2)15-6-9-18-21(12-15)32-22-13-16(27(3)4)7-10-19(22)23(18)17-8-5-14(24(28)29)11-20(17)25(30)31/h5-13H,1-4H3,(H-,28,29,30,31)
Quantity	25 mg, 250 mg, Bulk