Specifications
- Purity
- ≥97% (HPLC)
- Identity
- 1H-NMR
- Appearance
- Dark red powder
Properties
- Fluorescence
- λex 543 nm, λem 575 nm in 0.1 M Tris pH 8.0
- Melting Point
- 300 °C
- Solvents
- methanol, DMSO, DMF
Downloads
- Safety Data Sheet
- CDX C0055 MSDS.pdf
Available from stock
5-TAMRA
- SKU
- C0055
Category: Fluorescent Detection
- Synonyms
- 5-Carboxytetramethylrhodamine
- 91809-66-4
- CAS-Number
- C25H22N2O5
- Molecular Formula
- 430.45
- Molecular Weight
- Shipping
- AMBIENT
- Use / Stability
- Stable for at least 2 years after receipt when stored at +4°C.
- Handling Advice
- Protect from light and moisture.
- Long Term Storage
- +4°C
- Short Term Storage
- +4°C
- Transportation
- Not dangerous goods
- Description
- Free acid form of 5-carboxytetramethylrhodamine single isomer. Widely used fluorophore for preparing bioconjugates, especially fluorescent antibody and avidin derivatives used in immunochemistry. Used as a dye for oligonucleotide labeling and automated DNA sequencing applications.
- InChi
- InChI=1S/C25H22N2O5/c1-26(2)15-6-9-18-21(12-15)32-22-13-16(27(3)4)7-10-19(22)23(18)17-8-5-14(24(28)29)11-20(17)25(30)31/h5-13H,1-4H3,(H-,28,29,30,31)
- References
- (1) A. Aneja et al.: J. Biol. Phys. 34(5), 487 (2008) , (2) C. Dose et al.: Bioorg. Med. Chem. 16(1), 65 (2008) , (3) G. Vamosi et al.: Fluorescence characteristics. Biophys. J. 71, 972 (1996) , (4) L.G. Lee et al.: Nucleic Acids Res. 20, 2471 (1992)
- InChi Key
- YMZMTOFQCVHHFB-UHFFFAOYSA-N
- Smiles
- CN(C)C1=CC=C2C(OC3=CC(C=CC3=C2C2=CC=C(C=C2C([O-])=O)C(O)=O)=[N+](C)C)=C1
Free acid form of 5-carboxytetramethylrhodamine single isomer. Widely used fluorophore for preparing bioconjugates, especially fluorescent antibody and avidin derivatives used in immunochemistry. Used as a dye for oligonucleotide labeling and automated DNA sequencing applications.
Additional information
| Synonyms | 5-Carboxytetramethylrhodamine |
|---|---|
| Purity | ≥97% (HPLC) |
| Appearance | Dark red powder |
| CAS-Number | 91809-66-4 |
| Molecular Formula | C25H22N2O5 |
| Molecular Weight | 430.45 |
| Identity | 1H-NMR |
| Solvents | methanol, DMSO, DMF |
| Melting Point | 300 °C |
| Fluorescence | λex 543 nm, λem 575 nm in 0.1 M Tris pH 8.0 |
| Smiles | CN(C)C1=CC=C2C(OC3=CC(C=CC3=C2C2=CC=C(C=C2C([O-])=O)C(O)=O)=[N+](C)C)=C1 |
| InChi Key | YMZMTOFQCVHHFB-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | +4°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at +4°C. |
| Transportation | Not dangerous goods |
| References | (1) A. Aneja et al.: J. Biol. Phys. 34(5), 487 (2008), (2) C. Dose et al.: Bioorg. Med. Chem. 16(1), 65 (2008), (3) G. Vamosi et al.: Fluorescence characteristics. Biophys. J. 71, 972 (1996), (4) L.G. Lee et al.: Nucleic Acids Res. 20, 2471 (1992) |
| InChi | InChI=1S/C25H22N2O5/c1-26(2)15-6-9-18-21(12-15)32-22-13-16(27(3)4)7-10-19(22)23(18)17-8-5-14(24(28)29)11-20(17)25(30)31/h5-13H,1-4H3,(H-,28,29,30,31) |
| Quantity | 25 mg, 250 mg, Bulk |
