5(6)-FAM
- SKU
- C0045
Categories: Fluorescent Detection, Fluorescent Detection
- Synonyms
- 5(6)-Carboxyfluorescein
- 72088-94-9
- CAS-Number
- C21H12O7
- Molecular Formula
- 376.32
- Molecular Weight
Specifications
- Purity
- ≥95% (HPLC)
- Appearance
- Dark orange to brown solid
- Identity
- 1H-NMR
- Solubility
- Soluble in MeOH
Properties
- Solvents
- DMSO
- Melting Point
- 275 °C
- Fluorescence
- λex 492 nm, λem 517 nm in 0.1 M Tris pH 8.0
Downloads
- Safety Data Sheet
- CDX C0045 MSDS.pdf
- Shipping
- AMBIENT
- Short Term Storage
- +4°C
- Long Term Storage
- +4°C
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at +4°C.
- Transportation
- Not dangerous goods
- Description
- Carboxyfluorescein is commonly employed as a polar tracer and used for intracellular pH measurements. It has also been used for studying viability of various types of bacteria.
- Smiles
- C1=CC2=C(C=C1C(=O)O)C3(C4=C(C=C(C=C4)O)OC5=C3C=CC(=C5)O)OC2=O.C1=CC2=C(C=C1C(=O)O)C(=O)OC23C4=C(C=C(C=C4)O)OC5=C3C=CC(=C5)O
- InChi Key
- CPRVUBZYHZGASQ-UHFFFAOYSA-N
- References
- (1) M. Bradley et al.: Bioorg. Med. Chem. Lett. 18(1), 313 (2008) , (2) K. Hashizaki et al.: Chem. Pharm. Bull. 54(1), 80 (2006) , (3) M.L. Graber et al.: Anal. Biochem. 156, 202 (1986) , (4) D.F. Babcock et al.: J. Biol. Chem. 258, 6380 (1983)
- InChi
- InChI=1S/C20H12O5.C2H4O2/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20
Carboxyfluorescein is commonly employed as a polar tracer and used for intracellular pH measurements. It has also been used for studying viability of various types of bacteria.