AMCA-H

Specifications

Purity

≥90% (HPLC)

Identity

1H-NMR

Appearance

Brown solid

Properties

Fluorescence

λex 350 nm, λem 443 nm in methanol

Solvents

DMSO, DMF

Downloads

Safety Data Sheet

CDX A0009 MSDS.pdf

Certificate of Analysis

CDX A0009 CofA N6023_Vs3.PDF

Specification

CDX A0009 Specifications.pdf

1H-NMR

CDX A0009 NMR N6023.pdf

Handling Advice

Protect from light and moisture.

Use / Stability

Stable for at least 2 years after receipt when stored at -20°C.

Long Term Storage

-20°C

Short Term Storage

+4°C

Shipping

AMBIENT

Transportation

Not dangerous goods

Signal word

Warning

GHS Symbol

GHS07

Precautionary statements

P261, P305 + P351 + P338

Hazard statements

H315, H319, H335

Description

AMCA is one of the brightest amine-reactive blue fluorescent dyes useful for immunofluorescence and fluorescent labeling (Ex/Em: 353/455nm). It is quite photostable, and its fluorescence is pH-independent from pH 4 to 10. The properties include a relatively large Stoke's shift and resistance to photobleaching. Reactive AMCA derivatives are used as contrasting probes for double and triple labeling in immunofluorescence microscopy, arrays and in situ hybridization. NHS-AMCA and Sulfo-NHS-AMCA are reactive towards primary amine groups on antibodies, proteins, peptides and other biomolecules. AMCA-Hydrazide is used to label glycosylation sites or for conjugation to carboxyl groups using the crosslinker EDC.

InChi

InChI=1S/C12H11NO4/c1-6-8-3-2-7(13)4-10(8)17-12(16)9(6)5-11(14)15/h2-4H,5,13H2,1H3,(H,14,15)

References

(1) G-L. Ferri et al.: J. Histochem. Cytochem. 45(2), 155 (1997) , (2) B. Ufhake et al.: J. Neurosc. Meth. 40(1), 39 (1991) , (3) M. W. Wessendorf et al.: J. Histochem. Cytochem. 38(1), 87 (1990)

InChi Key

QEQDLKUMPUDNPG-UHFFFAOYSA-N

Smiles

CC1=C(CC(O)=O)C(=O)OC2=C1C=CC(N)=C2