- Synonyms: 7-Amino-4-methyl-3-coumarinylacetic acid
- Purity: ≥90% (HPLC)
- Appearance: Brown solid.
- CAS-Number: 106562-32-7
- Molecular Formula: C12H11NO4
- Molecular Weight: 233.22
- Identity: 1H-NMR
- Solvents: DMSO or DMF
- Fluorescence: λex 350 nm; λem 443 nm in methanol
- Smiles: CC1=C(CC(O)=O)C(=O)OC2=C1C=CC(N)=C2
- InChi: InChI=1S/C12H11NO4/c1-6-8-3-2-7(13)4-10(8)17-12(16)9(6)5-11(14)15/h2-4H,5,13H2,1H3,(H,14,15)
- InChi Key: QEQDLKUMPUDNPG-UHFFFAOYSA-N
Available from stock
AMCA-H
SKU: A0009
Category: Fluorescent Detection
- Shipping: AMBIENT
- Short Term Storage: +4°C
- Long Term Storage: -20°C
- Handling Advice: Protect from light and moisture.
- Use / Stability: Stable for at least 2 years after receipt when stored at -20°C.
- Description: AMCA is one of the brightest amine-reactive blue fluorescent dyes useful for immunofluorescence and fluorescent labeling (Ex/Em: 353/455nm). It is quite photostable, and its fluorescence is pH-independent from pH 4 to 10. The properties include a relatively large Stoke's shift and resistance to photobleaching. Reactive AMCA derivatives are used as contrasting probes for double and triple labeling in immunofluorescence microscopy, arrays and in situ hybridization. NHS-AMCA and Sulfo-NHS-AMCA are reactive towards primary amine groups on antibodies, proteins, peptides and other biomolecules. AMCA-Hydrazide is used to label glycosylation sites or for conjugation to carboxyl groups using the crosslinker EDC.
- References: (1) G-L. Ferri et al.; J. Histochem. Cytochem. 45(2), 155 (1997), (2) B. Ufhake et al.; J. Neurosc. Meth. 40(1), 39 (1991), (3) M. W. Wessendorf et al.; J. Histochem. Cytochem. 38(1), 87 (1990)
AMCA is one of the brightest amine-reactive blue fluorescent dyes useful for immunofluorescence and fluorescent labeling (Ex/Em: 353/455nm). It is quite photostable, and its fluorescence is pH-independent from pH 4 to 10. The properties include a relatively large Stoke’s shift and resistance to photobleaching. Reactive AMCA derivatives are used as contrasting probes for double and triple labeling in immunofluorescence microscopy, arrays and in situ hybridization. NHS-AMCA and Sulfo-NHS-AMCA are reactive towards primary amine groups on antibodies, proteins, peptides and other biomolecules. AMCA-Hydrazide is used to label glycosylation sites or for conjugation to carboxyl groups using the crosslinker EDC.
Additional information
| Identity | 1H-NMR |
|---|---|
| Handling Advice | Protect from light and moisture. |
| Availability | 1 |
| CAS-Number | 106562-32-7 |
| Long Term Storage | -20°C |
| InChi Key | QEQDLKUMPUDNPG-UHFFFAOYSA-N |
| Quantity | 100 mg, 2 g, 500 mg, Bulk |
| Appearance | Brown solid. |
| InChi | InChI=1S/C12H11NO4/c1-6-8-3-2-7(13)4-10(8)17-12(16)9(6)5-11(14)15/h2-4H,5,13H2,1H3,(H,14,15) |
| Fluorescence | λex 350 nm; λem 443 nm in methanol |
| Molecular Formula | |
| Short Term Storage | +4°C |
| Purity | |
| Pricetable | |
| References | (1) G-L. Ferri et al.; J. Histochem. Cytochem. 45(2), 155 (1997), (2) B. Ufhake et al.; J. Neurosc. Meth. 40(1), 39 (1991), (3) M. W. Wessendorf et al.; J. Histochem. Cytochem. 38(1), 87 (1990) |
| Smiles | CC1=C(CC(O)=O)C(=O)OC2=C1C=CC(N)=C2 |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Molecular Weight | |
| Solvents | DMSO or DMF |
| Synonyms | 7-Amino-4-methyl-3-coumarinylacetic acid |
| Shipping | AMBIENT |
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