AMCA-H

Available from stock

AMCA-H

SKU
A0009

Category:

Synonyms
7-Amino-4-methyl-3-coumarinylacetic acid
106562-32-7
CAS-Number
C12H11NO4
Molecular Formula
233.22
Molecular Weight

Specifications

Purity
≥90% (HPLC)
Identity
1H-NMR
Appearance
Brown solid

Properties

Fluorescence
λex 350 nm, λem 443 nm in methanol
Solvents
DMSO, DMF

Downloads

Safety Data Sheet
CDX A0009 MSDS.pdf
Certificate of Analysis
CDX A0009 CofA N6023_Vs3.PDF
Specification
CDX A0009 Specifications.pdf
1H-NMR
CDX A0009 NMR N6023.pdf
Handling Advice
Protect from light and moisture.
Use / Stability
Stable for at least 2 years after receipt when stored at -20°C.
Long Term Storage
-20°C
Short Term Storage
+4°C
Shipping
AMBIENT
Transportation
Not dangerous goods
Signal word
Warning
GHS Symbol
GHS07
Precautionary statements
P261, P305 + P351 + P338
Hazard statements
H315, H319, H335
Description
AMCA is one of the brightest amine-reactive blue fluorescent dyes useful for immunofluorescence and fluorescent labeling (Ex/Em: 353/455nm). It is quite photostable, and its fluorescence is pH-independent from pH 4 to 10. The properties include a relatively large Stoke's shift and resistance to photobleaching. Reactive AMCA derivatives are used as contrasting probes for double and triple labeling in immunofluorescence microscopy, arrays and in situ hybridization. NHS-AMCA and Sulfo-NHS-AMCA are reactive towards primary amine groups on antibodies, proteins, peptides and other biomolecules. AMCA-Hydrazide is used to label glycosylation sites or for conjugation to carboxyl groups using the crosslinker EDC.
InChi
InChI=1S/C12H11NO4/c1-6-8-3-2-7(13)4-10(8)17-12(16)9(6)5-11(14)15/h2-4H,5,13H2,1H3,(H,14,15)
References
(1) G-L. Ferri et al.: J. Histochem. Cytochem. 45(2), 155 (1997) , (2) B. Ufhake et al.: J. Neurosc. Meth. 40(1), 39 (1991) , (3) M. W. Wessendorf et al.: J. Histochem. Cytochem. 38(1), 87 (1990)
InChi Key
QEQDLKUMPUDNPG-UHFFFAOYSA-N
Smiles
CC1=C(CC(O)=O)C(=O)OC2=C1C=CC(N)=C2

AMCA is one of the brightest amine-reactive blue fluorescent dyes useful for immunofluorescence and fluorescent labeling (Ex/Em: 353/455nm). It is quite photostable, and its fluorescence is pH-independent from pH 4 to 10. The properties include a relatively large Stoke’s shift and resistance to photobleaching. Reactive AMCA derivatives are used as contrasting probes for double and triple labeling in immunofluorescence microscopy, arrays and in situ hybridization. NHS-AMCA and Sulfo-NHS-AMCA are reactive towards primary amine groups on antibodies, proteins, peptides and other biomolecules. AMCA-Hydrazide is used to label glycosylation sites or for conjugation to carboxyl groups using the crosslinker EDC.

SKU: A0009 Category:

Additional information

Synonyms

7-Amino-4-methyl-3-coumarinylacetic acid
Purity

≥90% (HPLC)
Appearance

Brown solid
CAS-Number

106562-32-7
Molecular Formula

C12H11NO4
Molecular Weight

233.22
Identity

1H-NMR
Solvents

DMSO, DMF
Fluorescence

λex 350 nm, λem 443 nm in methanol
Smiles

CC1=C(CC(O)=O)C(=O)OC2=C1C=CC(N)=C2
InChi Key

QEQDLKUMPUDNPG-UHFFFAOYSA-N
Shipping

AMBIENT
Short Term Storage

+4°C
Long Term Storage

-20°C
Handling Advice

Protect from light and moisture.
Use / Stability

Stable for at least 2 years after receipt when stored at -20°C.
Hazard statements

H315, H319, H335
Precautionary statements

P261, P305 + P351 + P338
GHS Symbol

GHS07
Signal word

Warning
Transportation

Not dangerous goods
References

(1) G-L. Ferri et al.: J. Histochem. Cytochem. 45(2), 155 (1997), (2) B. Ufhake et al.: J. Neurosc. Meth. 40(1), 39 (1991), (3) M. W. Wessendorf et al.: J. Histochem. Cytochem. 38(1), 87 (1990)
InChi

InChI=1S/C12H11NO4/c1-6-8-3-2-7(13)4-10(8)17-12(16)9(6)5-11(14)15/h2-4H,5,13H2,1H3,(H,14,15)
Quantity

100 mg, 500 mg, 2 g, Bulk

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