Specifications
- Purity
- ≥90% (HPLC)
- Identity
- 1H-NMR
- Appearance
- Brown solid
Properties
- Fluorescence
- λex 350 nm, λem 443 nm in methanol
- Solvents
- DMSO, DMF
Available from stock
AMCA-H
- SKU
- A0009
Category: Fluorescent Detection
- Synonyms
- 7-Amino-4-methyl-3-coumarinylacetic acid
- 106562-32-7
- CAS-Number
- C12H11NO4
- Molecular Formula
- 233.22
- Molecular Weight
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at -20°C.
- Long Term Storage
- -20°C
- Short Term Storage
- +4°C
- Shipping
- AMBIENT
- Transportation
- Not dangerous goods
- Signal word
- Warning
- GHS Symbol
- GHS07
- Precautionary statements
- P261, P305 + P351 + P338
- Hazard statements
- H315, H319, H335
- Description
- AMCA is one of the brightest amine-reactive blue fluorescent dyes useful for immunofluorescence and fluorescent labeling (Ex/Em: 353/455nm). It is quite photostable, and its fluorescence is pH-independent from pH 4 to 10. The properties include a relatively large Stoke's shift and resistance to photobleaching. Reactive AMCA derivatives are used as contrasting probes for double and triple labeling in immunofluorescence microscopy, arrays and in situ hybridization. NHS-AMCA and Sulfo-NHS-AMCA are reactive towards primary amine groups on antibodies, proteins, peptides and other biomolecules. AMCA-Hydrazide is used to label glycosylation sites or for conjugation to carboxyl groups using the crosslinker EDC.
- InChi
- InChI=1S/C12H11NO4/c1-6-8-3-2-7(13)4-10(8)17-12(16)9(6)5-11(14)15/h2-4H,5,13H2,1H3,(H,14,15)
- References
- (1) G-L. Ferri et al.: J. Histochem. Cytochem. 45(2), 155 (1997) , (2) B. Ufhake et al.: J. Neurosc. Meth. 40(1), 39 (1991) , (3) M. W. Wessendorf et al.: J. Histochem. Cytochem. 38(1), 87 (1990)
- InChi Key
- QEQDLKUMPUDNPG-UHFFFAOYSA-N
- Smiles
- CC1=C(CC(O)=O)C(=O)OC2=C1C=CC(N)=C2
AMCA is one of the brightest amine-reactive blue fluorescent dyes useful for immunofluorescence and fluorescent labeling (Ex/Em: 353/455nm). It is quite photostable, and its fluorescence is pH-independent from pH 4 to 10. The properties include a relatively large Stoke’s shift and resistance to photobleaching. Reactive AMCA derivatives are used as contrasting probes for double and triple labeling in immunofluorescence microscopy, arrays and in situ hybridization. NHS-AMCA and Sulfo-NHS-AMCA are reactive towards primary amine groups on antibodies, proteins, peptides and other biomolecules. AMCA-Hydrazide is used to label glycosylation sites or for conjugation to carboxyl groups using the crosslinker EDC.
Additional information
| Synonyms | 7-Amino-4-methyl-3-coumarinylacetic acid |
|---|---|
| Purity | ≥90% (HPLC) |
| Appearance | Brown solid |
| CAS-Number | 106562-32-7 |
| Molecular Formula | C12H11NO4 |
| Molecular Weight | 233.22 |
| Identity | 1H-NMR |
| Solvents | DMSO, DMF |
| Fluorescence | λex 350 nm, λem 443 nm in methanol |
| Smiles | CC1=C(CC(O)=O)C(=O)OC2=C1C=CC(N)=C2 |
| InChi Key | QEQDLKUMPUDNPG-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Hazard statements | H315, H319, H335 |
| Precautionary statements | P261, P305 + P351 + P338 |
| GHS Symbol | GHS07 |
| Signal word | Warning |
| Transportation | Not dangerous goods |
| References | (1) G-L. Ferri et al.: J. Histochem. Cytochem. 45(2), 155 (1997), (2) B. Ufhake et al.: J. Neurosc. Meth. 40(1), 39 (1991), (3) M. W. Wessendorf et al.: J. Histochem. Cytochem. 38(1), 87 (1990) |
| InChi | InChI=1S/C12H11NO4/c1-6-8-3-2-7(13)4-10(8)17-12(16)9(6)5-11(14)15/h2-4H,5,13H2,1H3,(H,14,15) |
| Quantity | 100 mg, 500 mg, 2 g, Bulk |
