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    AMCA-H

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    AMCA-H

    SKU
    A0009

    Category: Fluorescent Detection

    Synonyms
    7-Amino-4-methyl-3-coumarinylacetic acid
    106562-32-7
    CAS-Number
    C12H11NO4
    Molecular Formula
    233.22
    Molecular Weight

    • Product Data
    • Handling
    • Safety Information
    • Details

    Specifications

    Purity
    ≥90% (HPLC)
    Identity
    1H-NMR
    Appearance
    Brown solid

    Properties

    Fluorescence
    λex 350 nm, λem 443 nm in methanol
    Solvents
    DMSO, DMF

    Downloads

    Safety Data Sheet
    CDX A0009 MSDS.pdf
    Certificate of Analysis
    CDX A0009 CofA N6023_Vs3.PDF
    Specification
    CDX A0009 Specifications.pdf
    1H-NMR
    CDX A0009 NMR N6023.pdf
    Handling Advice
    Protect from light and moisture.
    Use / Stability
    Stable for at least 2 years after receipt when stored at -20°C.
    Long Term Storage
    -20°C
    Short Term Storage
    +4°C
    Shipping
    AMBIENT
    Transportation
    Not dangerous goods
    Signal word
    Warning
    GHS Symbol
    GHS07
    Precautionary statements
    P261, P305 + P351 + P338
    Hazard statements
    H315, H319, H335
    Description
    AMCA is one of the brightest amine-reactive blue fluorescent dyes useful for immunofluorescence and fluorescent labeling (Ex/Em: 353/455nm). It is quite photostable, and its fluorescence is pH-independent from pH 4 to 10. The properties include a relatively large Stoke's shift and resistance to photobleaching. Reactive AMCA derivatives are used as contrasting probes for double and triple labeling in immunofluorescence microscopy, arrays and in situ hybridization. NHS-AMCA and Sulfo-NHS-AMCA are reactive towards primary amine groups on antibodies, proteins, peptides and other biomolecules. AMCA-Hydrazide is used to label glycosylation sites or for conjugation to carboxyl groups using the crosslinker EDC.
    InChi
    InChI=1S/C12H11NO4/c1-6-8-3-2-7(13)4-10(8)17-12(16)9(6)5-11(14)15/h2-4H,5,13H2,1H3,(H,14,15)
    References
    (1) G-L. Ferri et al.: J. Histochem. Cytochem. 45(2), 155 (1997) , (2) B. Ufhake et al.: J. Neurosc. Meth. 40(1), 39 (1991) , (3) M. W. Wessendorf et al.: J. Histochem. Cytochem. 38(1), 87 (1990)
    InChi Key
    QEQDLKUMPUDNPG-UHFFFAOYSA-N
    Smiles
    CC1=C(CC(O)=O)C(=O)OC2=C1C=CC(N)=C2

    AMCA is one of the brightest amine-reactive blue fluorescent dyes useful for immunofluorescence and fluorescent labeling (Ex/Em: 353/455nm). It is quite photostable, and its fluorescence is pH-independent from pH 4 to 10. The properties include a relatively large Stoke’s shift and resistance to photobleaching. Reactive AMCA derivatives are used as contrasting probes for double and triple labeling in immunofluorescence microscopy, arrays and in situ hybridization. NHS-AMCA and Sulfo-NHS-AMCA are reactive towards primary amine groups on antibodies, proteins, peptides and other biomolecules. AMCA-Hydrazide is used to label glycosylation sites or for conjugation to carboxyl groups using the crosslinker EDC.

    SKU: A0009 Category: Fluorescent Detection
    • Additional information

    Additional information

    Synonyms

    7-Amino-4-methyl-3-coumarinylacetic acid

    Purity

    ≥90% (HPLC)

    Appearance

    Brown solid

    CAS-Number

    106562-32-7

    Molecular Formula

    C12H11NO4

    Molecular Weight

    233.22

    Identity

    1H-NMR

    Solvents

    DMSO, DMF

    Fluorescence

    λex 350 nm, λem 443 nm in methanol

    Smiles

    CC1=C(CC(O)=O)C(=O)OC2=C1C=CC(N)=C2

    InChi Key

    QEQDLKUMPUDNPG-UHFFFAOYSA-N

    Shipping

    AMBIENT

    Short Term Storage

    +4°C

    Long Term Storage

    -20°C

    Handling Advice

    Protect from light and moisture.

    Use / Stability

    Stable for at least 2 years after receipt when stored at -20°C.

    Hazard statements

    H315, H319, H335

    Precautionary statements

    P261, P305 + P351 + P338

    GHS Symbol

    GHS07

    Signal word

    Warning

    Transportation

    Not dangerous goods

    References

    (1) G-L. Ferri et al.: J. Histochem. Cytochem. 45(2), 155 (1997), (2) B. Ufhake et al.: J. Neurosc. Meth. 40(1), 39 (1991), (3) M. W. Wessendorf et al.: J. Histochem. Cytochem. 38(1), 87 (1990)

    InChi

    InChI=1S/C12H11NO4/c1-6-8-3-2-7(13)4-10(8)17-12(16)9(6)5-11(14)15/h2-4H,5,13H2,1H3,(H,14,15)

    Quantity

    100 mg, 500 mg, 2 g, Bulk

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