AMCA-H

Available from stock

AMCA-H

SKU
A0009

Categories: ,

Synonyms
7-Amino-4-methyl-3-coumarinylacetic acid
106562-32-7
CAS-Number
C12H11NO4
Molecular Formula
233.22
Molecular Weight

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Specifications

Purity
≥90% (HPLC)
Appearance
Brown solid
Identity
1H-NMR

Properties

Solvents
DMSO, DMF
Fluorescence
λex 350 nm, λem 443 nm in methanol

Documentation

Safety Data Sheet (SDS)
CDX A0009 MSDS.pdf
Certificate of Analysis (Example)
CDX A0009 CofA N6023_Vs3.PDF
Specification
CDX A0009 Specifications.pdf
Shipping
AMBIENT
Short Term Storage
+4°C
Long Term Storage
-20°C
Handling Advice
Protect from light and moisture.
Use / Stability
Stable for at least 2 years after receipt when stored at -20°C.
Hazard statements
H315, H319, H335
Precautionary statements
P261, P305 + P351 + P338
GHS Symbol
GHS07
Signal word
Warning
Transportation
Not dangerous goods
Description
AMCA is one of the brightest amine-reactive blue fluorescent dyes useful for immunofluorescence and fluorescent labeling (Ex/Em: 353/455nm). It is quite photostable, and its fluorescence is pH-independent from pH 4 to 10. The properties include a relatively large Stoke's shift and resistance to photobleaching. Reactive AMCA derivatives are used as contrasting probes for double and triple labeling in immunofluorescence microscopy, arrays and in situ hybridization. NHS-AMCA and Sulfo-NHS-AMCA are reactive towards primary amine groups on antibodies, proteins, peptides and other biomolecules. AMCA-Hydrazide is used to label glycosylation sites or for conjugation to carboxyl groups using the crosslinker EDC.
Smiles
CC1=C(CC(O)=O)C(=O)OC2=C1C=CC(N)=C2
InChi Key
QEQDLKUMPUDNPG-UHFFFAOYSA-N
References
(1) G-L. Ferri et al.: J. Histochem. Cytochem. 45(2), 155 (1997) , (2) B. Ufhake et al.: J. Neurosc. Meth. 40(1), 39 (1991) , (3) M. W. Wessendorf et al.: J. Histochem. Cytochem. 38(1), 87 (1990)
InChi
InChI=1S/C12H11NO4/c1-6-8-3-2-7(13)4-10(8)17-12(16)9(6)5-11(14)15/h2-4H,5,13H2,1H3,(H,14,15)

AMCA is one of the brightest amine-reactive blue fluorescent dyes useful for immunofluorescence and fluorescent labeling (Ex/Em: 353/455nm). It is quite photostable, and its fluorescence is pH-independent from pH 4 to 10. The properties include a relatively large Stoke’s shift and resistance to photobleaching. Reactive AMCA derivatives are used as contrasting probes for double and triple labeling in immunofluorescence microscopy, arrays and in situ hybridization. NHS-AMCA and Sulfo-NHS-AMCA are reactive towards primary amine groups on antibodies, proteins, peptides and other biomolecules. AMCA-Hydrazide is used to label glycosylation sites or for conjugation to carboxyl groups using the crosslinker EDC.

SKU: A0009 Categories: ,

Additional information

Synonyms

7-Amino-4-methyl-3-coumarinylacetic acid
Purity

≥90% (HPLC)
Appearance

Brown solid
CAS-Number

106562-32-7
Molecular Formula

C12H11NO4
Molecular Weight

233.22
Identity

1H-NMR
Solvents

DMSO, DMF
Fluorescence

λex 350 nm, λem 443 nm in methanol
Smiles

CC1=C(CC(O)=O)C(=O)OC2=C1C=CC(N)=C2
InChi Key

QEQDLKUMPUDNPG-UHFFFAOYSA-N
Shipping

AMBIENT
Short Term Storage

+4°C
Long Term Storage

-20°C
Handling Advice

Protect from light and moisture.
Use / Stability

Stable for at least 2 years after receipt when stored at -20°C.
Hazard statements

H315, H319, H335
Precautionary statements

P261, P305 + P351 + P338
GHS Symbol

GHS07
Signal word

Warning
Transportation

Not dangerous goods
References

(1) G-L. Ferri et al.: J. Histochem. Cytochem. 45(2), 155 (1997), (2) B. Ufhake et al.: J. Neurosc. Meth. 40(1), 39 (1991), (3) M. W. Wessendorf et al.: J. Histochem. Cytochem. 38(1), 87 (1990)
InChi

InChI=1S/C12H11NO4/c1-6-8-3-2-7(13)4-10(8)17-12(16)9(6)5-11(14)15/h2-4H,5,13H2,1H3,(H,14,15)
Quantity

100 mg, 500 mg, 2 g, Bulk

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