Specifications
- Purity
- ≥98% (HPLC)
- Appearance
- White to light brown powder
- Identity
- 1H-NMR
Properties
- Solvents
- Soluble in water.
- Fluorescence
- 512 ± 5 nm
Documentation
- Safety Data Sheet (SDS)
- CDX A0071 SDS_Vs1.1.pdf
Available from stock
ANTS
- SKU
- A0071
Category: Fluorescent Detection
- Synonyms
- 8-Aminonaphthalene-1,3,6-trisulfonic acid disodium salt, Disodium 8-amino-1,3,6-naphthalenetrisulfonate
- 5398-34-5
- CAS-Number
- C10H7NNa2O9S3
- Molecular Formula
- 427.34
- Molecular Weight
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
- Shipping
- AMBIENT
- Short Term Storage
- RT
- Long Term Storage
- RT
- Handling Advice
- Very hygroscopic. Store under inert gas.
- Use / Stability
- Stable for at least 2 years after receipt when stored at RT.
- Transportation
- Not dangerous goods
- Description
- ANTS is a highly negatively charged dye with an amino group that can be coupled to an aldehyde or ketone group to form an unstable Schiff base. The Schiff base is usually chemically reduced by sodium borohydride (NaBH4) or sodium cyanoborohydride (NaB(CN)H3) to form a stable linkage. This labeling technique has been widely used for the labeling and subsequent sequencing of oligosaccharides and glycoproteins. The negative charges of the dye facilitate the electrophoretic separation of the degradation products of carbohydrate polymers. The polyanionic dye ANTS is used together with the fluorescent cationic quencher DPX for membrane fusion studies or permeability assays. The mixture of ANTS-DPX is minimally fluorescent initially, but becomes increasingly more fluorescent upon dilution (i.e., membrane fusion or leakage). ANTS also is used as a neuronal tracer. ANTS has a relatively high Stokes shift in water, which sufficiently separates its emission from the majority of the autofluorescence of biological samples. Spectral Data: λex 356 nm, λem 512 nm in 0.1 M phosphate pH 7.0.
- Smiles
- NC1=C2C(C=C(S(=O)([O-])=O)C=C2S(=O)([O-])=O)=CC(S(=O)(O)=O)=C1.[Na+].[Na+]
- InChi Key
- KHJANRFXWPPWEL-UHFFFAOYSA-L
- References
- (1) H. Ellens, et al., Biochemistry 23, 1532 (1984) , (2) S.S. Basu, et al., Anal. Biochem. 222, 270 (1994) , (3) A. Klockow, et al., Electrophoresis 17, 110 (1996) , (4) M. Cabanes-Macheteau, et al., Electrophoresis 25, 8 (2004) , (5) K. Christopher, et al., MethodsX 2, 174 (2015)
- InChi
- InChI=1S/C10H9NO9S3.2Na/c11-8-3-6(21(12,13)14)1-5-2-7(22(15,16)17)4-9(10(5)8)23(18,19)20,,/h1-4H,11H2,(H,12,13,14)(H,15,16,17)(H,18,19,20),,/q,2*+1/p-2
ANTS is a highly negatively charged dye with an amino group that can be coupled to an aldehyde or ketone group to form an unstable Schiff base. The Schiff base is usually chemically reduced by sodium borohydride (NaBH4) or sodium cyanoborohydride (NaB(CN)H3) to form a stable linkage. This labeling technique has been widely used for the labeling and subsequent sequencing of oligosaccharides and glycoproteins. The negative charges of the dye facilitate the electrophoretic separation of the degradation products of carbohydrate polymers. The polyanionic dye ANTS is used together with the fluorescent cationic quencher DPX for membrane fusion studies or permeability assays. The mixture of ANTS-DPX is minimally fluorescent initially, but becomes increasingly more fluorescent upon dilution (i.e., membrane fusion or leakage). ANTS also is used as a neuronal tracer. ANTS has a relatively high Stokes shift in water, which sufficiently separates its emission from the majority of the autofluorescence of biological samples. Spectral Data: λex 356 nm, λem 512 nm in 0.1 M phosphate pH 7.0.
Additional information
| Synonyms | 8-Aminonaphthalene-1,3,6-trisulfonic acid disodium salt, Disodium 8-amino-1,3,6-naphthalenetrisulfonate |
|---|---|
| Purity | ≥98% (HPLC) |
| Appearance | White to light brown powder |
| CAS-Number | 5398-34-5 |
| Molecular Formula | C10H7NNa2O9S3 |
| Molecular Weight | 427.34 |
| Identity | 1H-NMR |
| Solvents | Soluble in water. |
| Fluorescence | 512 ± 5 nm |
| Smiles | NC1=C2C(C=C(S(=O)([O-])=O)C=C2S(=O)([O-])=O)=CC(S(=O)(O)=O)=C1.[Na+].[Na+] |
| InChi Key | KHJANRFXWPPWEL-UHFFFAOYSA-L |
| Shipping | AMBIENT |
| Short Term Storage | RT |
| Long Term Storage | RT |
| Handling Advice | Very hygroscopic. Store under inert gas. |
| Use / Stability | Stable for at least 2 years after receipt when stored at RT. |
| Transportation | Not dangerous goods |
| References | (1) H. Ellens, et al., Biochemistry 23, 1532 (1984), (2) S.S. Basu, et al., Anal. Biochem. 222, 270 (1994), (3) A. Klockow, et al., Electrophoresis 17, 110 (1996), (4) M. Cabanes-Macheteau, et al., Electrophoresis 25, 8 (2004), (5) K. Christopher, et al., MethodsX 2, 174 (2015) |
| InChi | InChI=1S/C10H9NO9S3.2Na/c11-8-3-6(21(12,13)14)1-5-2-7(22(15,16)17)4-9(10(5)8)23(18,19)20,,/h1-4H,11H2,(H,12,13,14)(H,15,16,17)(H,18,19,20),,/q,2*+1/p-2 |
| Quantity | 5 g, 10 g, Bulk |
