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ANTS

SKU: A0071

Categories: Fluorescent Detection, Fluorescent Detection

Synonyms: 8-Aminonaphthalene-1,3,6-trisulfonic acid disodium salt, Disodium 8-amino-1,3,6-naphthalenetrisulfonate; 5398-34-5
CAS-Number: C₁₀H₇NNa₂O₉S₃
Molecular Formula: 427.34
Molecular Weight

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥98% (HPLC)
Appearance: White to light brown powder
Identity: 1H-NMR

Properties

Solvents: Soluble in water.
Fluorescence: 512 ± 5 nm

Shipping: AMBIENT
Short Term Storage: RT
Long Term Storage: RT
Handling Advice: Very hygroscopic. Store under inert gas.
Use / Stability: Stable for at least 2 years after receipt when stored at RT.

Transportation: Not dangerous goods

Description: ANTS is a highly negatively charged dye with an amino group that can be coupled to an aldehyde or ketone group to form an unstable Schiff base. The Schiff base is usually chemically reduced by sodium borohydride (NaBH4) or sodium cyanoborohydride (NaB(CN)H3) to form a stable linkage. This labeling technique has been widely used for the labeling and subsequent sequencing of oligosaccharides and glycoproteins. The negative charges of the dye facilitate the electrophoretic separation of the degradation products of carbohydrate polymers. The polyanionic dye ANTS is used together with the fluorescent cationic quencher DPX for membrane fusion studies or permeability assays. The mixture of ANTS-DPX is minimally fluorescent initially, but becomes increasingly more fluorescent upon dilution (i.e., membrane fusion or leakage). ANTS also is used as a neuronal tracer. ANTS has a relatively high Stokes shift in water, which sufficiently separates its emission from the majority of the autofluorescence of biological samples. Spectral Data: λex 356 nm; λem 512 nm in 0.1 M phosphate pH 7.0.
Smiles: NC1=C2C(C=C(S(=O)([O-])=O)C=C2S(=O)([O-])=O)=CC(S(=O)(O)=O)=C1.[Na+].[Na+]
InChi Key: KHJANRFXWPPWEL-UHFFFAOYSA-L
References: (1) H. Ellens, et al.; Biochemistry 23, 1532 (1984) , (2) S.S. Basu, et al.; Anal. Biochem. 222, 270 (1994) , (3) A. Klockow, et al.; Electrophoresis 17, 110 (1996) , (4) M. Cabanes-Macheteau, et al.; Electrophoresis 25, 8 (2004) , (5) K. Christopher, et al.; MethodsX 2, 174 (2015)
InChi: (1) G-L. Ferri et al.: J. Histochem. Cytochem. 45(2), 155 (1997) , (2) B. Ufhake et al.: J. Neurosc. Meth. 40(1), 39 (1991) , (3) M. W. Wessendorf et al.: J. Histochem. Cytochem. 38(1), 87 (1990)

ANTS is a highly negatively charged dye with an amino group that can be coupled to an aldehyde or ketone group to form an unstable Schiff base. The Schiff base is usually chemically reduced by sodium borohydride (NaBH4) or sodium cyanoborohydride (NaB(CN)H3) to form a stable linkage. This labeling technique has been widely used for the labeling and subsequent sequencing of oligosaccharides and glycoproteins. The negative charges of the dye facilitate the electrophoretic separation of the degradation products of carbohydrate polymers. The polyanionic dye ANTS is used together with the fluorescent cationic quencher DPX for membrane fusion studies or permeability assays. The mixture of ANTS-DPX is minimally fluorescent initially, but becomes increasingly more fluorescent upon dilution (i.e., membrane fusion or leakage). ANTS also is used as a neuronal tracer. ANTS has a relatively high Stokes shift in water, which sufficiently separates its emission from the majority of the autofluorescence of biological samples. Spectral Data: λex 356 nm; λem 512 nm in 0.1 M phosphate pH 7.0.

SKU: A0071 Categories: Fluorescent Detection, Fluorescent Detection

Additional information

Additional information

Synonyms	8-Aminonaphthalene-1,3,6-trisulfonic acid disodium salt, Disodium 8-amino-1,3,6-naphthalenetrisulfonate
Purity	≥98% (HPLC)
Appearance	White to light brown powder
CAS-Number	5398-34-5
Molecular Formula	C10H7NNa2O9S3
Molecular Weight	427.34
Identity	1H-NMR
Solvents	Soluble in water.
Fluorescence	512 ± 5 nm
Smiles	NC1=C2C(C=C(S(=O)([O-])=O)C=C2S(=O)([O-])=O)=CC(S(=O)(O)=O)=C1.[Na+].[Na+]
InChi Key	KHJANRFXWPPWEL-UHFFFAOYSA-L
Shipping	AMBIENT
Short Term Storage	RT
Long Term Storage	RT
Handling Advice	Very hygroscopic. Store under inert gas.
Use / Stability	Stable for at least 2 years after receipt when stored at RT.
Transportation	Not dangerous goods
References	(1) H. Ellens, et al.; Biochemistry 23, 1532 (1984), (2) S.S. Basu, et al.; Anal. Biochem. 222, 270 (1994), (3) A. Klockow, et al.; Electrophoresis 17, 110 (1996), (4) M. Cabanes-Macheteau, et al.; Electrophoresis 25, 8 (2004), (5) K. Christopher, et al.; MethodsX 2, 174 (2015)
InChi	(1) G-L. Ferri et al.: J. Histochem. Cytochem. 45(2), 155 (1997), (2) B. Ufhake et al.: J. Neurosc. Meth. 40(1), 39 (1991), (3) M. W. Wessendorf et al.: J. Histochem. Cytochem. 38(1), 87 (1990)
Quantity	5 g, 10 g, Bulk