Available from stock

BCIP p-toluidine salt

SKU: B0024

Category: Fluorescent Detection

Synonyms: 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt, X-phosphate p-toluidine salt; 6578-06-9
CAS-Number: C₈H₆BrClNO₄P · C₇H₉N
Molecular Formula: 433.62
Molecular Weight

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥95% (HPLC)
Appearance: Beige powder
Identity: 1H-NMR

Properties

Solvents: DMSO (25mg/ml), DMF (25mg/ml), methanol

Shipping: AMBIENT
Short Term Storage: RT
Long Term Storage: +4°C
Handling Advice: Protect from light and moisture.
Use / Stability: Stable for at least 2 years after receipt when stored at +4°C.

Transportation: Not dangerous goods

Description: BCIP is a colorimetric substrate for alkaline phosphatase. Upon enzymatic cleavage by alkaline phosphatase, an indoxyl intermediate is released and subsequently oxidized to produce water-insoluble indigo. It can be used to detect alkaline phosphatase activity by immunohistochemistry (IHC) or Western blot (WB).
Smiles: BrC1=CC=C(NC=C2OP([O-])(O)=O)C2=C1Cl.[NH3+]C3=CC=C(C)C=C3
InChi Key: QEIFSLUFHRCVQL-UHFFFAOYSA-N
References: (1) E. Epstein, et al., Am. J. Clin. Pathol. 48, 530 (1967) , (2) P.L. Wolf, et al., Experientia 23, 183 (1967) , (3) P.L. Wolf, et al., Enzymologia 35, 154 (1968) , (4) C.-I. Chang, et al., Sensors 12, 2710 (2012) , (5) Y. Li, et al., Anal. Sci. 32, 215 (2016)

BCIP is a colorimetric substrate for alkaline phosphatase. Upon enzymatic cleavage by alkaline phosphatase, an indoxyl intermediate is released and subsequently oxidized to produce water-insoluble indigo. It can be used to detect alkaline phosphatase activity by immunohistochemistry (IHC) or Western blot (WB).

SKU: B0024 Category: Fluorescent Detection

Additional information

Additional information

Synonyms	5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt, X-phosphate p-toluidine salt
Purity	≥95% (HPLC)
Appearance	Beige powder
CAS-Number	6578-06-9
Molecular Formula	C8H6BrClNO4P · C7H9N
Molecular Weight	433.62
Identity	1H-NMR
Solvents	DMSO (25mg/ml), DMF (25mg/ml), methanol
Smiles	BrC1=CC=C(NC=C2OP([O-])(O)=O)C2=C1Cl.[NH3+]C3=CC=C(C)C=C3
InChi Key	QEIFSLUFHRCVQL-UHFFFAOYSA-N
Shipping	AMBIENT
Short Term Storage	RT
Long Term Storage	+4°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at +4°C.
Transportation	Not dangerous goods
References	(1) E. Epstein, et al., Am. J. Clin. Pathol. 48, 530 (1967), (2) P.L. Wolf, et al., Experientia 23, 183 (1967), (3) P.L. Wolf, et al., Enzymologia 35, 154 (1968), (4) C.-I. Chang, et al., Sensors 12, 2710 (2012), (5) Y. Li, et al., Anal. Sci. 32, 215 (2016)
Quantity	100 mg, 1 g, Bulk