Specifications
- Purity
- ≥95% (HPLC)
- Appearance
- Powder
- Identity
- 1H-NMR
Properties
- Solvents
- Soluble in DMSO (25mg/ml) or DMF (25mg/ml).
Category: Fluorescent Detection, Fluorescent Detection
BCIP is a colorimetric substrate for alkaline phosphatase. Upon enzymatic cleavage by alkaline phosphatase, an indoxyl intermediate is released and subsequently oxidized to produce water-insoluble indigo. It can be used to detect alkaline phosphatase activity by immunohistochemistry (IHC) or Western blot (WB).
Synonyms | 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt, X-phosphate p-toluidine salt |
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Purity | ≥95% (HPLC) |
Appearance | Powder |
CAS-Number | 6578-06-9 |
Molecular Formula | C8H6BrClNO4P · C7H9N |
Molecular Weight | 433.62 |
Identity | 1H-NMR |
Solvents | Soluble in DMSO (25mg/ml) or DMF (25mg/ml). |
Smiles | BrC1=CC=C(NC=C2OP([O-])(O)=O)C2=C1Cl.[NH3+]C3=CC=C(C)C=C3 |
InChi Key | QEIFSLUFHRCVQL-UHFFFAOYSA-N |
Shipping | AMBIENT |
Short Term Storage | RT |
Long Term Storage | +4°C |
Handling Advice | Protect from light and moisture. |
Use / Stability | Stable for at least 2 years after receipt when stored at +4°C. |
Transportation | Not dangerous goods |
References | (1) E. Epstein, et al., Am. J. Clin. Pathol. 48, 530 (1967), (2) P.L. Wolf, et al., Experientia 23, 183 (1967), (3) P.L. Wolf, et al., Enzymologia 35, 154 (1968), (4) C.-I. Chang, et al., Sensors 12, 2710 (2012), (5) Y. Li, et al., Anal. Sci. 32, 215 (2016) |
Quantity | 100 mg, 1 g, Bulk |