Biotinyl tyramide

SKU: B0270

Category: Fluorescent Detection

Synonyms: Biotin-Phenol , BP , N-(4-Hydroxyphenethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide; 41994-02-9
CAS-Number: C₁₈H₂₅N₃O₃S
Molecular Formula: 363.5
Molecular Weight

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥97% (HPLC)
Identity: 1H-NMR
Appearance: White solid

Properties

Solvents: acetonitrile, DMSO

Downloads

Safety Data Sheet: CDX B0270 MSDS.pdf

Short Term Storage: +4°C
Use / Stability: Stable for at least 2 years after receipt when stored at -20°C.
Handling Advice: Protect from light and moisture.
Long Term Storage: -20°C
Shipping: AMBIENT

Transportation: Not dangerous goods

Description: Biotin derivative. Substrate of the horseradish peroxidase enzyme and used as a reagent to amplify immunohistochemical signals. It is based on the HRP-catalyzed deposition of tyramide conjugates (such as biotinyl-tyramide) on a solid phase. Subsequent reaction with streptavidin fluorophore results in the localization of the fluorophore at the site of tyramide deposition. This fluorescence-based tyramide signal amplification (TSA) has been widely used in immunohistochemistry, immunohistochemistry, immunoelectron microscopy, fluorescent in situ hybridization (FISH) and fluorescence ELISA. The TSA method has been reported to increase the detection sensitivity up to 100-fold as compared with conventional avidin–biotinylated enzyme complex procedures. It can be used together with both chromogenic and fluorescence visualization methods. It can be added to any other standard IHC protocol and reduces the use of other reagents, improves signal to noise by reducing the titer of other reagents in the assay protocol and enables multi-target detection in both IHC and (F)ISH applications.
InChi: InChI=1S/C18H25N3O3S/c22-13-7-5-12(6-8-13)9-10-19-16(23)4-2-1-3-15-17-14(11-25-15)20-18(24)21-17/h5-8,14-15,17,22H,1-4,9-11H2,(H,19,23)(H2,20,21,24)/t14-,15-,17-/m0/s1
References: (1) B. Hunyady, et al.: J. Histochem. Cytochem. 44, 1353 (1996) , (2) G. Mayer, et al.: J. Histochem. Cytochem. 45, 1449 (1997) , (3) J.A. McKay, et al.: Mol. Path. 50, 322 (1997) , (4) M.F. Evans, et al.: Mod. Pathol. 15, 1339 (2002) , (5) Q. Chenac, et al.: Anal. Lett. 45, 219 (2012) , (6) H. Gong, et al.: Anal. Bioch. 426, 27 (2012) , (7) E. Draberova, et al.: J. Immunol. Methods 395, 63 (2013)
InChi Key: VZWXNOBHWODXCW-ZOBUZTSGSA-N
Smiles: [H][C@]12CS[C@@H](CCCCC(=O)NCCC3=CC=C(O)C=C3)[C@@]1([H])NC(=O)N2

Biotin derivative. Substrate of the horseradish peroxidase enzyme and used as a reagent to amplify immunohistochemical signals. It is based on the HRP-catalyzed deposition of tyramide conjugates (such as biotinyl-tyramide) on a solid phase. Subsequent reaction with streptavidin fluorophore results in the localization of the fluorophore at the site of tyramide deposition. This fluorescence-based tyramide signal amplification (TSA) has been widely used in immunohistochemistry, immunohistochemistry, immunoelectron microscopy, fluorescent in situ hybridization (FISH) and fluorescence ELISA. The TSA method has been reported to increase the detection sensitivity up to 100-fold as compared with conventional avidin–biotinylated enzyme complex procedures. It can be used together with both chromogenic and fluorescence visualization methods. It can be added to any other standard IHC protocol and reduces the use of other reagents, improves signal to noise by reducing the titer of other reagents in the assay protocol and enables multi-target detection in both IHC and (F)ISH applications.

SKU: B0270 Category: Fluorescent Detection

Additional information

Additional information

Synonyms	Biotin-Phenol, BP, N-(4-Hydroxyphenethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide
Purity	≥97% (HPLC)
Appearance	White solid
CAS-Number	41994-02-9
Molecular Formula	C18H25N3O3S
Molecular Weight	363.5
Identity	1H-NMR
Solvents	acetonitrile, DMSO
Smiles	[H][C@]12CS[C@@H](CCCCC(=O)NCCC3=CC=C(O)C=C3)[C@@]1([H])NC(=O)N2
InChi Key	VZWXNOBHWODXCW-ZOBUZTSGSA-N
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at -20°C.
Transportation	Not dangerous goods
References	(1) B. Hunyady, et al.: J. Histochem. Cytochem. 44, 1353 (1996), (2) G. Mayer, et al.: J. Histochem. Cytochem. 45, 1449 (1997), (3) J.A. McKay, et al.: Mol. Path. 50, 322 (1997), (4) M.F. Evans, et al.: Mod. Pathol. 15, 1339 (2002), (5) Q. Chenac, et al.: Anal. Lett. 45, 219 (2012), (6) H. Gong, et al.: Anal. Bioch. 426, 27 (2012), (7) E. Draberova, et al.: J. Immunol. Methods 395, 63 (2013)
InChi	InChI=1S/C18H25N3O3S/c22-13-7-5-12(6-8-13)9-10-19-16(23)4-2-1-3-15-17-14(11-25-15)20-18(24)21-17/h5-8,14-15,17,22H,1-4,9-11H2,(H,19,23)(H2,20,21,24)/t14-,15-,17-/m0/s1
Quantity	100 mg, 500 mg, Bulk