- Synonyms: Biotin-Phenol, BP, N-(4-Hydroxyphenethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide
- Purity: ≥97% (HPLC)
- Appearance: White to light pink solid.
- CAS-Number: 41994-02-9
- Molecular Formula: C18H25N3O3S
- Molecular Weight: 363.5
- Identity: 1H-NMR
- Solvents: acetonitrile, DMSO
- Melting Point: improves signal to noise by reducing the titer of
- Smiles: [H][C@]12CS[C@@H](CCCCC(=O)NCCC3=CC=C(O)C=C3)[C@@]1([H])NC(=O)N2
- InChi: InChI=1S/C18H25N3O3S/c22-13-7-5-12(6-8-13)9-10-19-16(23)4-2-1-3-15-17-14(11-25-15)20-18(24)21-17/h5-8,14-15,17,22H,1-4,9-11H2,(H,19,23)(H2,20,21,24)/t14-,15-,17-/m0/s1
- InChi Key: VZWXNOBHWODXCW-ZOBUZTSGSA-N
Available from stock
Biotinyl tyramide
SKU: B0270
Category: Fluorescent Detection
- Shipping: AMBIENT
- Short Term Storage: +4°C
- Long Term Storage: -20°C
- Handling Advice: Protect from light and moisture. Keep cool and dry.
- Use / Stability: Stable for at least 2 years after receipt when stored at -20°C.
- Description: Biotin derivative. Substrate of the horseradish peroxidase enzyme and used as a reagent to amplify immunohistochemical signals. It is based on the HRP-catalyzed deposition of tyramide conjugates (such as biotinyl-tyramide) on a solid phase. Subsequent reaction with streptavidin fluorophore results in the localization of the fluorophore at the site of tyramide deposition. This fluorescence-based tyramide signal amplification (TSA) has been widely used in immunohistochemistry, immunohistochemistry, immunoelectron microscopy, fluorescent in situ hybridization (FISH) and fluorescence ELISA. The TSA method has been reported to increase the detection sensitivity up to 100-fold as compared with conventional avidin–biotinylated enzyme complex procedures. It can be used together with both chromogenic and fluorescence visualization methods. It can be added to any other standard IHC protocol and reduces the use of other reagents, improves signal to noise by reducing the titer of other reagents in the assay protocol and enables multi-target detection in both IHC and (F)ISH applications.
- References: (1) B. Hunyady, et al.; J. Histochem. Cytochem. 44, 1353 (1996), (2) G. Mayer, et al.; J. Histochem. Cytochem. 45, 1449 (1997), (3) J.A. McKay, et al.; Mol. Path. 50, 322 (1997), (4) M.F. Evans, et al.; Mod. Pathol. 15, 1339 (2002), (5) Q. Chenac, et al.; Anal. Lett. 45, 219 (2012), (6) H. Gong, et al.; Anal. Bioch. 426, 27 (2012), (7) E. Draberova, et al.; J. Immunol. Methods 395, 63 (2013)
Biotin derivative. Substrate of the horseradish peroxidase enzyme and used as a reagent to amplify immunohistochemical signals. It is based on the HRP-catalyzed deposition of tyramide conjugates (such as biotinyl-tyramide) on a solid phase. Subsequent reaction with streptavidin fluorophore results in the localization of the fluorophore at the site of tyramide deposition. This fluorescence-based tyramide signal amplification (TSA) has been widely used in immunohistochemistry, immunohistochemistry, immunoelectron microscopy, fluorescent in situ hybridization (FISH) and fluorescence ELISA. The TSA method has been reported to increase the detection sensitivity up to 100-fold as compared with conventional avidin–biotinylated enzyme complex procedures. It can be used together with both chromogenic and fluorescence visualization methods. It can be added to any other standard IHC protocol and reduces the use of other reagents, improves signal to noise by reducing the titer of other reagents in the assay protocol and enables multi-target detection in both IHC and (F)ISH applications.
Additional information
| InChi | InChI=1S/C18H25N3O3S/c22-13-7-5-12(6-8-13)9-10-19-16(23)4-2-1-3-15-17-14(11-25-15)20-18(24)21-17/h5-8,14-15,17,22H,1-4,9-11H2,(H,19,23)(H2,20,21,24)/t14-,15-,17-/m0/s1 |
|---|---|
| Quantity | 100 mg, 500 mg, Bulk |
| Identity | 1H-NMR |
| Availability | 1 |
| Molecular Formula | |
| Long Term Storage | -20°C |
| Melting Point | improves signal to noise by reducing the titer of |
| Appearance | White to light pink solid. |
| InChi Key | VZWXNOBHWODXCW-ZOBUZTSGSA-N |
| CAS-Number | 41994-02-9 |
| Handling Advice | Protect from light and moisture. Keep cool and dry. |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Pricetable | |
| References | (1) B. Hunyady, et al.; J. Histochem. Cytochem. 44, 1353 (1996), (2) G. Mayer, et al.; J. Histochem. Cytochem. 45, 1449 (1997), (3) J.A. McKay, et al.; Mol. Path. 50, 322 (1997), (4) M.F. Evans, et al.; Mod. Pathol. 15, 1339 (2002), (5) Q. Chenac, et al.; Anal. Lett. 45, 219 (2012), (6) H. Gong, et al.; Anal. Bioch. 426, 27 (2012), (7) E. Draberova, et al.; J. Immunol. Methods 395, 63 (2013) |
| Smiles | [H][C@]12CS[C@@H](CCCCC(=O)NCCC3=CC=C(O)C=C3)[C@@]1([H])NC(=O)N2 |
| Solvents | acetonitrile, DMSO |
| Molecular Weight | |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Purity | |
| Synonyms | Biotin-Phenol, BP, N-(4-Hydroxyphenethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide |
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