Specifications
- Purity
- ≥97% (HPLC)
- Appearance
- White solid
- Identity
- 1H-NMR
Properties
- Solvents
- acetonitrile, DMSO
Documentation
- Safety Data Sheet (SDS)
- CDX B0270 MSDS.pdf
Available from stock
Biotinyl tyramide
- SKU
- B0270
Categories: Fluorescent Detection, Fluorescent Detection
- Synonyms
- Biotin-Phenol , BP , N-(4-Hydroxyphenethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide
- 41994-02-9
- CAS-Number
- C18H25N3O3S
- Molecular Formula
- 363.5
- Molecular Weight
- Shipping
- AMBIENT
- Short Term Storage
- +4°C
- Long Term Storage
- -20°C
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at -20°C.
- Transportation
- Not dangerous goods
- Description
- Biotin derivative. Substrate of the horseradish peroxidase enzyme and used as a reagent to amplify immunohistochemical signals. It is based on the HRP-catalyzed deposition of tyramide conjugates (such as biotinyl-tyramide) on a solid phase. Subsequent reaction with streptavidin fluorophore results in the localization of the fluorophore at the site of tyramide deposition. This fluorescence-based tyramide signal amplification (TSA) has been widely used in immunohistochemistry, immunohistochemistry, immunoelectron microscopy, fluorescent in situ hybridization (FISH) and fluorescence ELISA. The TSA method has been reported to increase the detection sensitivity up to 100-fold as compared with conventional avidin–biotinylated enzyme complex procedures. It can be used together with both chromogenic and fluorescence visualization methods. It can be added to any other standard IHC protocol and reduces the use of other reagents, improves signal to noise by reducing the titer of other reagents in the assay protocol and enables multi-target detection in both IHC and (F)ISH applications.
- Smiles
- [H][C@]12CS[C@@H](CCCCC(=O)NCCC3=CC=C(O)C=C3)[C@@]1([H])NC(=O)N2
- InChi Key
- VZWXNOBHWODXCW-ZOBUZTSGSA-N
- References
- (1) B. Hunyady, et al.: J. Histochem. Cytochem. 44, 1353 (1996) , (2) G. Mayer, et al.: J. Histochem. Cytochem. 45, 1449 (1997) , (3) J.A. McKay, et al.: Mol. Path. 50, 322 (1997) , (4) M.F. Evans, et al.: Mod. Pathol. 15, 1339 (2002) , (5) Q. Chenac, et al.: Anal. Lett. 45, 219 (2012) , (6) H. Gong, et al.: Anal. Bioch. 426, 27 (2012) , (7) E. Draberova, et al.: J. Immunol. Methods 395, 63 (2013)
- InChi
- InChI=1S/C18H25N3O3S/c22-13-7-5-12(6-8-13)9-10-19-16(23)4-2-1-3-15-17-14(11-25-15)20-18(24)21-17/h5-8,14-15,17,22H,1-4,9-11H2,(H,19,23)(H2,20,21,24)/t14-,15-,17-/m0/s1
Biotin derivative. Substrate of the horseradish peroxidase enzyme and used as a reagent to amplify immunohistochemical signals. It is based on the HRP-catalyzed deposition of tyramide conjugates (such as biotinyl-tyramide) on a solid phase. Subsequent reaction with streptavidin fluorophore results in the localization of the fluorophore at the site of tyramide deposition. This fluorescence-based tyramide signal amplification (TSA) has been widely used in immunohistochemistry, immunohistochemistry, immunoelectron microscopy, fluorescent in situ hybridization (FISH) and fluorescence ELISA. The TSA method has been reported to increase the detection sensitivity up to 100-fold as compared with conventional avidin–biotinylated enzyme complex procedures. It can be used together with both chromogenic and fluorescence visualization methods. It can be added to any other standard IHC protocol and reduces the use of other reagents, improves signal to noise by reducing the titer of other reagents in the assay protocol and enables multi-target detection in both IHC and (F)ISH applications.
Additional information
| Synonyms | Biotin-Phenol, BP, N-(4-Hydroxyphenethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide |
|---|---|
| Purity | ≥97% (HPLC) |
| Appearance | White solid |
| CAS-Number | 41994-02-9 |
| Molecular Formula | C18H25N3O3S |
| Molecular Weight | 363.5 |
| Identity | 1H-NMR |
| Solvents | acetonitrile, DMSO |
| Smiles | [H][C@]12CS[C@@H](CCCCC(=O)NCCC3=CC=C(O)C=C3)[C@@]1([H])NC(=O)N2 |
| InChi Key | VZWXNOBHWODXCW-ZOBUZTSGSA-N |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Transportation | Not dangerous goods |
| References | (1) B. Hunyady, et al.: J. Histochem. Cytochem. 44, 1353 (1996), (2) G. Mayer, et al.: J. Histochem. Cytochem. 45, 1449 (1997), (3) J.A. McKay, et al.: Mol. Path. 50, 322 (1997), (4) M.F. Evans, et al.: Mod. Pathol. 15, 1339 (2002), (5) Q. Chenac, et al.: Anal. Lett. 45, 219 (2012), (6) H. Gong, et al.: Anal. Bioch. 426, 27 (2012), (7) E. Draberova, et al.: J. Immunol. Methods 395, 63 (2013) |
| InChi | InChI=1S/C18H25N3O3S/c22-13-7-5-12(6-8-13)9-10-19-16(23)4-2-1-3-15-17-14(11-25-15)20-18(24)21-17/h5-8,14-15,17,22H,1-4,9-11H2,(H,19,23)(H2,20,21,24)/t14-,15-,17-/m0/s1 |
| Quantity | 100 mg, 500 mg, Bulk |
