Specifications
- Purity
- ≥96% (HPLC)
- Appearance
- White solid
- Identity
- 1H-NMR
Properties
- Solvents
- DMSO or DMF
- Fluorescence
- λex 470 nm, λem 529 nm in 0.1 M Tris pH 8.0, esterase
Available from stock
Carboxy-DCFDA N-succinimidyl ester
- SKU
- C0059
Categories: Fluorescent Detection, Fluorescent Detection
- Synonyms
- 5(6)-Carboxy-2',7'-dichlorofluorescein diacetate N-succinimidyl ester
- 147265-60-9
- CAS-Number
- C29H17Cl2NO11
- Molecular Formula
- 626.35
- Molecular Weight
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
- Shipping
- AMBIENT
- Short Term Storage
- +4°C
- Long Term Storage
- -20°C
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at -20°C.
- Transportation
- Not dangerous goods
- Description
- 5-(and-6)-Carboxy-2',7'-dichlorofluorescein diacetate, succinimidyl ester is a useful fluorescent tracer that can passively diffuse into cells and covalently label intracellular proteins, resulting in long-term cell labeling. The reagent itself is colorless and nonfluorescent but becomes brightly green fluorescent once it is hydrolyzed by intracellular esterases.
- Smiles
- CC(=O)ON1C(=O)CCC1=O.CC(=O)OC1=CC2=C(C=C1Cl)C1(OC(=O)C3=C1C=CC=C3)C1=CC(Cl)=C(OC(C)=O)C=C1O2
- InChi Key
- KPBFEPKORAGIIW-UHFFFAOYSA-N
- References
- (1) C.M. Griffith et al.: Development 116, 1087 (1992)
- InChi
- InChI=1S/C25H16Cl2O6.C6H7NO4/c1-12(28)31-22-8-14-7-15-9-23(32-13(2)29)21(27)11-19(15)25(18(14)10-20(22)26)17-6-4-3-5-16(17)24(30)33-25
5-(and-6)-Carboxy-2′,7′-dichlorofluorescein diacetate, succinimidyl ester is a useful fluorescent tracer that can passively diffuse into cells and covalently label intracellular proteins, resulting in long-term cell labeling. The reagent itself is colorless and nonfluorescent but becomes brightly green fluorescent once it is hydrolyzed by intracellular esterases.
Additional information
| Synonyms | 5(6)-Carboxy-2',7'-dichlorofluorescein diacetate N-succinimidyl ester |
|---|---|
| Purity | ≥96% (HPLC) |
| Appearance | White solid |
| CAS-Number | 147265-60-9 |
| Molecular Formula | C29H17Cl2NO11 |
| Molecular Weight | 626.35 |
| Identity | 1H-NMR |
| Solvents | DMSO or DMF |
| Fluorescence | λex 470 nm, λem 529 nm in 0.1 M Tris pH 8.0, esterase |
| Smiles | CC(=O)ON1C(=O)CCC1=O.CC(=O)OC1=CC2=C(C=C1Cl)C1(OC(=O)C3=C1C=CC=C3)C1=CC(Cl)=C(OC(C)=O)C=C1O2 |
| InChi Key | KPBFEPKORAGIIW-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Transportation | Not dangerous goods |
| References | (1) C.M. Griffith et al.: Development 116, 1087 (1992) |
| InChi | InChI=1S/C25H16Cl2O6.C6H7NO4/c1-12(28)31-22-8-14-7-15-9-23(32-13(2)29)21(27)11-19(15)25(18(14)10-20(22)26)17-6-4-3-5-16(17)24(30)33-25 |
| Quantity | 50 mg, 250 mg, Bulk |
