Carboxy-DCFDA N-succinimidyl ester

SKU: C0059

Category: Fluorescent Detection

Synonyms: 5(6)-Carboxy-2',7'-dichlorofluorescein diacetate N-succinimidyl ester; 147265-60-9
CAS-Number: C₂₉H₁₇Cl₂NO₁₁
Molecular Formula: 626.35
Molecular Weight

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥96% (HPLC)
Identity: 1H-NMR
Appearance: White solid

Properties

Fluorescence: λex 470 nm, λem 529 nm in 0.1 M Tris pH 8.0, esterase
Solvents: DMSO or DMF

Short Term Storage: +4°C
Use / Stability: Stable for at least 2 years after receipt when stored at -20°C.
Handling Advice: Protect from light and moisture.
Long Term Storage: -20°C
Shipping: AMBIENT

Transportation: Not dangerous goods

Description: 5-(and-6)-Carboxy-2',7'-dichlorofluorescein diacetate, succinimidyl ester is a useful fluorescent tracer that can passively diffuse into cells and covalently label intracellular proteins, resulting in long-term cell labeling. The reagent itself is colorless and nonfluorescent but becomes brightly green fluorescent once it is hydrolyzed by intracellular esterases.
InChi: InChI=1S/C25H16Cl2O6.C6H7NO4/c1-12(28)31-22-8-14-7-15-9-23(32-13(2)29)21(27)11-19(15)25(18(14)10-20(22)26)17-6-4-3-5-16(17)24(30)33-25
References: (1) C.M. Griffith et al.: Development 116, 1087 (1992)
InChi Key: KPBFEPKORAGIIW-UHFFFAOYSA-N
Smiles: CC(=O)ON1C(=O)CCC1=O.CC(=O)OC1=CC2=C(C=C1Cl)C1(OC(=O)C3=C1C=CC=C3)C1=CC(Cl)=C(OC(C)=O)C=C1O2

5-(and-6)-Carboxy-2′,7′-dichlorofluorescein diacetate, succinimidyl ester is a useful fluorescent tracer that can passively diffuse into cells and covalently label intracellular proteins, resulting in long-term cell labeling. The reagent itself is colorless and nonfluorescent but becomes brightly green fluorescent once it is hydrolyzed by intracellular esterases.

SKU: C0059 Category: Fluorescent Detection

Additional information

Additional information

Synonyms	5(6)-Carboxy-2',7'-dichlorofluorescein diacetate N-succinimidyl ester
Purity	≥96% (HPLC)
Appearance	White solid
CAS-Number	147265-60-9
Molecular Formula	C29H17Cl2NO11
Molecular Weight	626.35
Identity	1H-NMR
Solvents	DMSO or DMF
Fluorescence	λex 470 nm, λem 529 nm in 0.1 M Tris pH 8.0, esterase
Smiles	CC(=O)ON1C(=O)CCC1=O.CC(=O)OC1=CC2=C(C=C1Cl)C1(OC(=O)C3=C1C=CC=C3)C1=CC(Cl)=C(OC(C)=O)C=C1O2
InChi Key	KPBFEPKORAGIIW-UHFFFAOYSA-N
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at -20°C.
Transportation	Not dangerous goods
References	(1) C.M. Griffith et al.: Development 116, 1087 (1992)
InChi	InChI=1S/C25H16Cl2O6.C6H7NO4/c1-12(28)31-22-8-14-7-15-9-23(32-13(2)29)21(27)11-19(15)25(18(14)10-20(22)26)17-6-4-3-5-16(17)24(30)33-25
Quantity	50 mg, 250 mg, Bulk