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    Carboxy-DCFDA N-succinimidyl ester

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      Carboxy-DCFDA N-succinimidyl ester

      SKU
      C0059

      Categories: Fluorescent Detection, Fluorescent Detection

      Synonyms
      5(6)-Carboxy-2',7'-dichlorofluorescein diacetate N-succinimidyl ester
      147265-60-9
      CAS-Number
      C29H17Cl2NO11
      Molecular Formula
      626.35
      Molecular Weight

      For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

      • Product Data
      • Handling
      • Safety Information
      • Details

      Specifications

      Purity
      ≥96% (HPLC)
      Appearance
      White solid
      Identity
      1H-NMR

      Properties

      Solvents
      DMSO or DMF
      Fluorescence
      λex 470 nm, λem 529 nm in 0.1 M Tris pH 8.0, esterase
      Shipping
      AMBIENT
      Short Term Storage
      +4°C
      Long Term Storage
      -20°C
      Handling Advice
      Protect from light and moisture.
      Use / Stability
      Stable for at least 2 years after receipt when stored at -20°C.
      Transportation
      Not dangerous goods
      Description
      5-(and-6)-Carboxy-2',7'-dichlorofluorescein diacetate, succinimidyl ester is a useful fluorescent tracer that can passively diffuse into cells and covalently label intracellular proteins, resulting in long-term cell labeling. The reagent itself is colorless and nonfluorescent but becomes brightly green fluorescent once it is hydrolyzed by intracellular esterases.
      Smiles
      CC(=O)ON1C(=O)CCC1=O.CC(=O)OC1=CC2=C(C=C1Cl)C1(OC(=O)C3=C1C=CC=C3)C1=CC(Cl)=C(OC(C)=O)C=C1O2
      InChi Key
      KPBFEPKORAGIIW-UHFFFAOYSA-N
      References
      (1) C.M. Griffith et al.: Development 116, 1087 (1992)
      InChi
      InChI=1S/C25H16Cl2O6.C6H7NO4/c1-12(28)31-22-8-14-7-15-9-23(32-13(2)29)21(27)11-19(15)25(18(14)10-20(22)26)17-6-4-3-5-16(17)24(30)33-25

      5-(and-6)-Carboxy-2′,7′-dichlorofluorescein diacetate, succinimidyl ester is a useful fluorescent tracer that can passively diffuse into cells and covalently label intracellular proteins, resulting in long-term cell labeling. The reagent itself is colorless and nonfluorescent but becomes brightly green fluorescent once it is hydrolyzed by intracellular esterases.

      SKU: C0059 Categories: Fluorescent Detection, Fluorescent Detection
      • Additional information

      Additional information

      Synonyms

      5(6)-Carboxy-2',7'-dichlorofluorescein diacetate N-succinimidyl ester

      Purity

      ≥96% (HPLC)

      Appearance

      White solid

      CAS-Number

      147265-60-9

      Molecular Formula

      C29H17Cl2NO11

      Molecular Weight

      626.35

      Identity

      1H-NMR

      Solvents

      DMSO or DMF

      Fluorescence

      λex 470 nm, λem 529 nm in 0.1 M Tris pH 8.0, esterase

      Smiles

      CC(=O)ON1C(=O)CCC1=O.CC(=O)OC1=CC2=C(C=C1Cl)C1(OC(=O)C3=C1C=CC=C3)C1=CC(Cl)=C(OC(C)=O)C=C1O2

      InChi Key

      KPBFEPKORAGIIW-UHFFFAOYSA-N

      Shipping

      AMBIENT

      Short Term Storage

      +4°C

      Long Term Storage

      -20°C

      Handling Advice

      Protect from light and moisture.

      Use / Stability

      Stable for at least 2 years after receipt when stored at -20°C.

      Transportation

      Not dangerous goods

      References

      (1) C.M. Griffith et al.: Development 116, 1087 (1992)

      InChi

      InChI=1S/C25H16Cl2O6.C6H7NO4/c1-12(28)31-22-8-14-7-15-9-23(32-13(2)29)21(27)11-19(15)25(18(14)10-20(22)26)17-6-4-3-5-16(17)24(30)33-25

      Quantity

      50 mg, 250 mg, Bulk

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