Specifications
- Purity
- ≥98% (HPLC)
- Appearance
- Yellow powder
- Identity
- 1H-NMR
Properties
- Solvents
- Soluble in chloroform or methanol.
Available from stock
Dansyl fluoride
- SKU
- D0241
Category: Fluorescent Detection
- Synonyms
- 5-(Dimethylamino)-1-naphthalenesulfonyl fluoride, DNSF
- 34523-28-9
- CAS-Number
- C12H12FNO2S
- Molecular Formula
- 253.3
- Molecular Weight
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
- Shipping
- AMBIENT
- Short Term Storage
- +4°C
- Long Term Storage
- +4°C
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at +4°C.
- Hazard statements
- H314
- Precautionary statements
- P280-P305 + P351 + P338-P310
- GHS Symbol
- GHS05
- Signal word
- Danger
- RIDADR
- UN3261
- Transportation
- Packing Group II
- Description
- Dansyl fluoride is a fluorogenic reagent used as a specific fluorescent probe for serine proteases and is a serine-type protease inhibitor. It is an active site directed fluorescent inhibitor of guanidinobenzoatase (GB), which binds to both the colonic isoenzymic forms of the GB. The GB on cell surfaces after binding dansyl fluoride makes the cells fluoresce blue. It has been used for the location of tumour cells in frozen sections of human lung squamous cell carcinoma and colonic carcinoma tissues. It is used as a precursor for the synthesis of other derivatives like dansyl amide. Spectral data: &lambda,ex 360nm, &lambda,em 580nm (methanol).
- Smiles
- CN(C)C1=C(C=CC=C2S(=O)(F)=O)C2=CC=C1
- InChi Key
- JMHHECQPPFEVMU-UHFFFAOYSA-N
- References
- (1) W.L Vaz & G. Schoellmann, Biochim. Biophys. Acta 439, 194 (1976) , (2) J. Stackhouse & F.H. Westheimer, J. Org. Chem. 46, 1891 (1981) , (3) G. Schoellmann, et al., Biochim. Biophys. Acta 704, 403 (1982) , (4) R.F. Chen & C.H. Scott, Anal. Lett. 18, 393 (1985) , (5) M. Anees & E.W. Benbow, J. Enzyme Inhib. 10, 195 (1996) , (6) R. Zhou, et al., PNAS 95, 4959 (1998)
- InChi
- InChI=1S/C12H12FNO2S/c1-14(2)11-7-3-6-10-9(11)5-4-8-12(10)17(13,15)16/h3-8H,1-2H3
Dansyl fluoride is a fluorogenic reagent used as a specific fluorescent probe for serine proteases and is a serine-type protease inhibitor. It is an active site directed fluorescent inhibitor of guanidinobenzoatase (GB), which binds to both the colonic isoenzymic forms of the GB. The GB on cell surfaces after binding dansyl fluoride makes the cells fluoresce blue. It has been used for the location of tumour cells in frozen sections of human lung squamous cell carcinoma and colonic carcinoma tissues. It is used as a precursor for the synthesis of other derivatives like dansyl amide. Spectral data: &lambda,ex 360nm, &lambda,em 580nm (methanol).
Additional information
| Synonyms | 5-(Dimethylamino)-1-naphthalenesulfonyl fluoride, DNSF |
|---|---|
| Purity | ≥98% (HPLC) |
| Appearance | Yellow powder |
| CAS-Number | 34523-28-9 |
| Molecular Formula | C12H12FNO2S |
| Molecular Weight | 253.3 |
| Identity | 1H-NMR |
| Solvents | Soluble in chloroform or methanol. |
| Smiles | CN(C)C1=C(C=CC=C2S(=O)(F)=O)C2=CC=C1 |
| InChi Key | JMHHECQPPFEVMU-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | +4°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at +4°C. |
| Hazard statements | H314 |
| Precautionary statements | P280-P305 + P351 + P338-P310 |
| GHS Symbol | GHS05 |
| Signal word | Danger |
| RIDADR | UN3261 |
| Transportation | Packing Group II |
| References | (1) W.L Vaz & G. Schoellmann, Biochim. Biophys. Acta 439, 194 (1976), (2) J. Stackhouse & F.H. Westheimer, J. Org. Chem. 46, 1891 (1981), (3) G. Schoellmann, et al., Biochim. Biophys. Acta 704, 403 (1982), (4) R.F. Chen & C.H. Scott, Anal. Lett. 18, 393 (1985), (5) M. Anees & E.W. Benbow, J. Enzyme Inhib. 10, 195 (1996), (6) R. Zhou, et al., PNAS 95, 4959 (1998) |
| InChi | InChI=1S/C12H12FNO2S/c1-14(2)11-7-3-6-10-9(11)5-4-8-12(10)17(13,15)16/h3-8H,1-2H3 |
| Quantity | 250 mg, Bulk |
