Available from stock

Dansyl fluoride

SKU: D0241

Categories: Fluorescent Detection, Fluorescent Detection

Synonyms: 5-(Dimethylamino)-1-naphthalenesulfonyl fluoride, DNSF; 34523-28-9
CAS-Number: C₁₂H₁₂FNO₂S
Molecular Formula: 253.3
Molecular Weight

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥98% (HPLC)
Appearance: Yellow powder
Identity: 1H-NMR

Properties

Solvents: Soluble in chloroform or methanol.

Shipping: AMBIENT
Short Term Storage: +4°C
Long Term Storage: +4°C
Handling Advice: Protect from light and moisture.
Use / Stability: Stable for at least 2 years after receipt when stored at +4°C.

Hazard statements: H314
Precautionary statements: P280-P305 + P351 + P338-P310
GHS Symbol: GHS05
Signal word: Danger
RIDADR: UN3261
Transportation: Packing Group II

Description: D-Desthiobiotin is a protein cross-linking agent and a precursor in the synthesis of biotin. Desthiobiotin is a modified form of biotin that binds less tightly to avidin and streptavidin than biotin while still providing excellent specificity in affinity purification methods. Unlike biomolecules that are labeled with biotin, proteins and other targets that are labeled with desthiobiotin can be eluted without harsh, denaturing conditions. D-Desthiobiotin is used in affinity chromatography and protein chromatography, and can be used for protein and cell labeling, detection and isolation. It has been used in the preparation of agarose matrices for affinity-based isolation of streptavidin-fluorophore conjugates. Desthiobiotin is an efficient competitor for the mild elution of Strep-tag®II and TwinStrep-tag® fusion proteins from Strep-Tactin® columns. For elution 2.5 mM desthiobiotin is added to the buffer.
Smiles: CN(C)C1=C(C=CC=C2S(=O)(F)=O)C2=CC=C1
InChi Key: JMHHECQPPFEVMU-UHFFFAOYSA-N
References: (1) J.D. Hirsch, et al.; Anal. Biochem. 308, 343 (2002) , (2) R. Rodriguez-Melendez, et al.; J. Nutr. 133, 1259 (2003) , (3) S.C. Wu & S.L. Wong; Anal. Biochem. 331, 340 (2004) , (4) P. Guillaume, et al.; J. Immunol. 177, 3903 (2006) , (5) T.G.M. Schmidt, et al.; Protein Expr.Purif. 92, 54 (2013)
InChi: (1) G. Chen, et al.: Protein Sci. 8, 2151 (1999) , (2) M.K. Thorson, et al.: Angew. Chem. Int. Ed. Engl. 52, 4641 (2013) , (3) B. Chen, et al.: Analyst. 138, 946 (2013)

D-Desthiobiotin is a protein cross-linking agent and a precursor in the synthesis of biotin. Desthiobiotin is a modified form of biotin that binds less tightly to avidin and streptavidin than biotin while still providing excellent specificity in affinity purification methods. Unlike biomolecules that are labeled with biotin, proteins and other targets that are labeled with desthiobiotin can be eluted without harsh, denaturing conditions. D-Desthiobiotin is used in affinity chromatography and protein chromatography, and can be used for protein and cell labeling, detection and isolation. It has been used in the preparation of agarose matrices for affinity-based isolation of streptavidin-fluorophore conjugates. Desthiobiotin is an efficient competitor for the mild elution of Strep-tag®II and TwinStrep-tag® fusion proteins from Strep-Tactin® columns. For elution 2.5 mM desthiobiotin is added to the buffer.

SKU: D0241 Categories: Fluorescent Detection, Fluorescent Detection

Additional information

Additional information

Synonyms	5-(Dimethylamino)-1-naphthalenesulfonyl fluoride, DNSF
Purity	≥98% (HPLC)
Appearance	Yellow powder
CAS-Number	34523-28-9
Molecular Formula	C12H12FNO2S
Molecular Weight	253.3
Identity	1H-NMR
Solvents	Soluble in chloroform or methanol.
Smiles	CN(C)C1=C(C=CC=C2S(=O)(F)=O)C2=CC=C1
InChi Key	JMHHECQPPFEVMU-UHFFFAOYSA-N
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	+4°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at +4°C.
Hazard statements	H314
Precautionary statements	P280-P305 + P351 + P338-P310
GHS Symbol	GHS05
Signal word	Danger
RIDADR	UN3261
Transportation	Packing Group II
References	(1) J.D. Hirsch, et al.; Anal. Biochem. 308, 343 (2002), (2) R. Rodriguez-Melendez, et al.; J. Nutr. 133, 1259 (2003), (3) S.C. Wu & S.L. Wong; Anal. Biochem. 331, 340 (2004), (4) P. Guillaume, et al.; J. Immunol. 177, 3903 (2006), (5) T.G.M. Schmidt, et al.; Protein Expr.Purif. 92, 54 (2013)
InChi	(1) G. Chen, et al.: Protein Sci. 8, 2151 (1999), (2) M.K. Thorson, et al.: Angew. Chem. Int. Ed. Engl. 52, 4641 (2013), (3) B. Chen, et al.: Analyst. 138, 946 (2013)
Quantity	250 mg, Bulk