Specifications
- Purity
- ≥97% (HPLC)
- Appearance
- Solid
- Identity
- 1H-NMR
Properties
- Solvents
- DMSO
- Fluorescence
- λex 500 nm, λem ~593 nm in 0.1 M Tris pH 8.0 (after cleavage by esterase)
Available from stock
Resorufin-isobutyrate
- SKU
- I0005
Categories: Fluorescent Detection, Fluorescent Detection
- Synonyms
- 7-(Isobutyloxycarbonyloxy)-3H-phenoxazin-3-one
- 251292-24-7
- CAS-Number
- C17H15NO5
- Molecular Formula
- 313.3
- Molecular Weight
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
- Shipping
- AMBIENT
- Short Term Storage
- +4°C
- Long Term Storage
- -20°C
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at -20°C.
- Transportation
- Not dangerous goods
- Description
- Cell permeable resorufin derivative used as a fluorogenic indicator for cell viability. Resorufin was selected as the fluorophore because it fluoresces at a longer wavelength and is amenable to modification at the hydroxyl group which is essential for its fluorescence. Incubation with esterase at pH 8.0 results in a 80-90 nm shift of emission ≤ (Ex/Em: 500/~593nm in 0.1 M Tris pH 8.0 (after cleavage by esterase)). This cell viability indicator is 2 times more sensitive than calcein-AM.
- Smiles
- CC(C)COC(=O)OC1=CC2=C(C=C1)N=C1C=CC(=O)C=C1O2
- InChi Key
- RVDFSVVDUVHHKE-UHFFFAOYSA-N
- References
- (1) M. Ishiyama et al.: Analyt. Sci. 15, 1025 (1999)
- InChi
- InChI=1S/C17H15NO5/c1-10(2)9-21-17(20)22-12-4-6-14-16(8-12)23-15-7-11(19)3-5-13(15)18-14/h3-8,10H,9H2,1-2H3
Cell permeable resorufin derivative used as a fluorogenic indicator for cell viability. Resorufin was selected as the fluorophore because it fluoresces at a longer wavelength and is amenable to modification at the hydroxyl group which is essential for its fluorescence. Incubation with esterase at pH 8.0 results in a 80-90 nm shift of emission ≤ (Ex/Em: 500/~593nm in 0.1 M Tris pH 8.0 (after cleavage by esterase)). This cell viability indicator is 2 times more sensitive than calcein-AM.
Additional information
| Synonyms | 7-(Isobutyloxycarbonyloxy)-3H-phenoxazin-3-one |
|---|---|
| Purity | ≥97% (HPLC) |
| Appearance | Solid |
| CAS-Number | 251292-24-7 |
| Molecular Formula | C17H15NO5 |
| Molecular Weight | 313.3 |
| Identity | 1H-NMR |
| Solvents | DMSO |
| Fluorescence | λex 500 nm, λem ~593 nm in 0.1 M Tris pH 8.0 (after cleavage by esterase) |
| Smiles | CC(C)COC(=O)OC1=CC2=C(C=C1)N=C1C=CC(=O)C=C1O2 |
| InChi Key | RVDFSVVDUVHHKE-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Transportation | Not dangerous goods |
| References | (1) M. Ishiyama et al.: Analyt. Sci. 15, 1025 (1999) |
| InChi | InChI=1S/C17H15NO5/c1-10(2)9-21-17(20)22-12-4-6-14-16(8-12)23-15-7-11(19)3-5-13(15)18-14/h3-8,10H,9H2,1-2H3 |
| Quantity | 1 mg, 5 mg, 25 mg, Bulk |
