- ≥97% (HPLC)
- λex 500 nm, λem ~593 nm in 0.1 M Tris pH 8.0 (after cleavage by esterase)
Category: Fluorescent Detection
Cell permeable resorufin derivative used as a fluorogenic indicator for cell viability. Resorufin was selected as the fluorophore because it fluoresces at a longer wavelength and is amenable to modification at the hydroxyl group which is essential for its fluorescence. Incubation with esterase at pH 8.0 results in a 80-90 nm shift of emission ≤ (Ex/Em: 500/~593nm in 0.1 M Tris pH 8.0 (after cleavage by esterase)). This cell viability indicator is 2 times more sensitive than calcein-AM.
λex 500 nm, λem ~593 nm in 0.1 M Tris pH 8.0 (after cleavage by esterase)
|Short Term Storage|
|Long Term Storage|
Protect from light and moisture.
|Use / Stability|
Stable for at least 2 years after receipt when stored at -20°C.
Not dangerous goods
(1) M. Ishiyama et al.: Analyt. Sci. 15, 1025 (1999)
1 mg, 5 mg, 25 mg, Bulk