Specifications
- Purity
- ≥97% (NMR)
- Appearance
- White to off-white powder
- Identity
- 1H-NMR
Properties
- Solvents
- DMSO (20 mg/ml), DMF(20 mg/ml), methanol
Biotin-X-NHS is an amino-reactive biotinylation reagent with a long spacer arm. The 6-aminocaproic acid spacer arm provides distance between the biotin and the target ligand which helps alleviate steric hindrance. Biotin-X-NHS is used to biotinylate antibodies, proteins, peptides, or amino acids by reacting with primary amines as found in the N-terminus and Lysine side chains. The Biotin-X-NHS molecule is cell membrane permeable and can be used to label intracellular proteins. Optimal reaction conditions are at a pH of 7-9. Avoid amine-containing buffers, which may compete in the acylation reaction.
Synonyms | NHS-LC-Biotin, Biotinamidohexanoic acid N-hydroxysuccinimide ester, (+)-Biotinamidocaproate N-hydroxysuccinimidyl ester, N-(+)-Biotinyl-6-aminocaproic acid N-succinimidyl ester, N-(+)-Biotinyl-6-aminohexanoic acid NHS ester, Enzotin |
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Purity | ≥97% (NMR) |
Appearance | White to off-white powder |
CAS-Number | 72040-63-2 |
Molecular Formula | C20H30N4O6S |
Molecular Weight | 454.54 |
Identity | 1H-NMR |
Solvents | DMSO (20 mg/ml), DMF(20 mg/ml), methanol |
Smiles | O=C(CCCCC1SCC2NC(=O)NC12)NCCCCCC(=O)ON1C(=O)CCC1=O |
InChi Key | UVGHPGOONBRLCX-NJSLBKSFSA-N |
Shipping | AMBIENT |
Short Term Storage | -20°C |
Long Term Storage | -20°C |
Handling Advice | Protect from light and moisture. |
Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
Transportation | Not dangerous goods |
References | (1) G.T. Hermanson, Bioconjugate Techn. 2nd p506-545 (2008) |
Quantity | 25 mg, 100 mg, Bulk |