4-Methylumbelliferyl ß-D-glucuronide dihydrate
- SKU
- M0076
Category: Fluorescent Detection, Fluorescent Detection
- Synonyms
- 4-Methylumbelliferyl-ß-D-glucopyranosiduronic acid , MUG , 4-MUG , 4-MU-glcUA , MUGlcU , 6160-80-1 (anhydrous)
- 6160-80-1
- CAS-Number
- C16H16O9 · xH2O
- Molecular Formula
- 352.29
- Molecular Weight
Specifications
- Purity
- ≥99% (HPLC)
- Appearance
- Colourless to white crystalline powder
- Identity
- 1H-NMR
Properties
- Solvents
- DMSO (50 mg/ml) or water.
- Melting Point
- 140-143 °C
- Optical Activity
- -116 ° (0.25 g/100 mL in water; wavlen 589.3 nm)
- Density
- 1.632 g/mL (predicted)
- Fluorescence
- λEx 365 nm, lamEm 445 nm in 0.1 M phosphate pH 6.5
- Shipping
- AMBIENT
- Short Term Storage
- +4°C
- Long Term Storage
- +4°C
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at +4°C.
- Transportation
- Not dangerous goods
- Description
- 4-Methylumbelliferyl-β-D-glucuronic acid dihydrate (MUG) is a non-fluorescent but fluorogenic β-glucuronidase substrate. Once cleaved, MUG yields blue fluorescence seen under UV light. The substrate is commonly used for rapid sensitive identification of Escherichia coli contamination and for detecting marker GUS gene expression in plants with high sensitivity. In microbiology it is a component in Violet Red Bile Agar and Lauryl Tryptose Broth, for the detection of coliform organisms in water, food and dairy products. Spectral Data. λEx/λEm= 360/450 nm (cleaved product). MUG both directly and indirectly inhibits hyaluronan (HA) synthesis.
- Smiles
- O[C@@H]1[C@@H](O)[C@H](O)[C@@H](C(O)=O)O[C@H]1OC2=CC(OC(C=C3C)=O)=C3C=C2
- InChi Key
- ARQXEQLMMNGFDU-JHZZJYKESA-N
- References
- (1) P.C. Feng & P.A. Hartman, Appl. Environ. Microbiol. 43, 1320 (1982) , (2) B. Sperker, et al., J. Chromatogr. B Biomed. Sci. Appl. 685, 181 (1996) , (3) P. Villari, et al., Lett. Appl. Microbiol. 24, 286 (1997) , (4) S. Fior, et al., Plant Sci. 176, 130 (2009) , (5) N. Nagy, et al., JBC 294, 7864 (2019)
- InChi
- InChI=1S/C16H16O9/c1-6-4-10(17)24-9-5-7(2-3-8(6)9)23-16-13(20)11(18)12(19)14(25-16)15(21)22/h2-5,11-14,16,18-20H,1H3,(H,21,22)/t11-,12-,13+,14-,16+/m0/s1
4-Methylumbelliferyl-β-D-glucuronic acid dihydrate (MUG) is a non-fluorescent but fluorogenic β-glucuronidase substrate. Once cleaved, MUG yields blue fluorescence seen under UV light. The substrate is commonly used for rapid sensitive identification of Escherichia coli contamination and for detecting marker GUS gene expression in plants with high sensitivity. In microbiology it is a component in Violet Red Bile Agar and Lauryl Tryptose Broth, for the detection of coliform organisms in water, food and dairy products. Spectral Data. λEx/λEm= 360/450 nm (cleaved product). MUG both directly and indirectly inhibits hyaluronan (HA) synthesis.