DAR-4M solution (5 mM in DMSO), 1 mg in 0.46 ml DMSO

SKU: D0506

Category: Fluorescent Detection

Synonyms: Diaminorhodamine-4M , 3,6-Bis(dimethylamino)-9-[3-amino-4-(N-methylamino)-2-carboxyphenyl]xanthylium; 339527-79-6
CAS-Number: C₂₅H₂₆N₄O₃
Molecular Formula: 430.51
Molecular Weight

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥98% (HPLC)
Identity: 1H-NMR
Appearance: Reddish-violet liquid

Properties

Boiling Point: 219°C
Melting Point: 219°C
Solvents: DMSO

Long Term Storage: -20°C
Use / Stability: Stable for at least 2 years after receipt when stored at -20°C.
Handling Advice: Protect from light and moisture.
Short Term Storage: +4°C
Shipping: AMBIENT

Transportation: Not dangerous goods
Signal word: Warning
GHS Symbol: GHS07
Precautionary statements: P261-P305 + P351 + P338
Hazard statements: H315-H319-H335

Description: Photo-stable fluorescent probe (Ex: ~560nm, Em: 575nm) for the detection of nitric oxide (NO) in presence of oxygen, resulting in a triazolo-rhodamine analog (DAR-4M T) that exhibits about 840-fold greater fluorescence quantum efficiency. Suitable probe for monitoring RNS production, but not NO alone. Stable in a pH range of 4-12. Detection limit ~10nM.
InChi: InChI=1S/C25H26N4O3/c1-27-19-11-10-18(23(24(19)26)25(30)31)22-16-8-6-14(28(2)3)12-20(16)32-21-13-15(29(4)5)7-9-17(21)22/h6-13,27H,26H2,1-5H3
References: (1) H. Kojima, et al., Anal. Chem.73, 1967 (2001) , (2) H. Kojima & N. Nagano, Advanced Materials 12, 763 (2000) , (3) H. Kojima, et al., Tetrahedr. Lett. 41, 69 (2000) , (4) Z. Lacza, et al., J. Pharmacol. Toxicol. Meth. 52, 335 (2005)
InChi Key: LNDSYTUQQCXPAM-UHFFFAOYSA-N
Smiles: CN(C)C1=CC2=[O+]C(C=C(N(C)C)C=C3)=C3C(C4=CC=C(NC)C(N)=C4C([O-])=O)=C2C=C1

Photo-stable fluorescent probe (Ex: ~560nm, Em: 575nm) for the detection of nitric oxide (NO) in presence of oxygen, resulting in a triazolo-rhodamine analog (DAR-4M T) that exhibits about 840-fold greater fluorescence quantum efficiency. Suitable probe for monitoring RNS production, but not NO alone. Stable in a pH range of 4-12. Detection limit ~10nM.

SKU: D0506 Category: Fluorescent Detection

Additional information

Additional information

Synonyms	Diaminorhodamine-4M, 3,6-Bis(dimethylamino)-9-[3-amino-4-(N-methylamino)-2-carboxyphenyl]xanthylium
Purity	≥98% (HPLC)
Appearance	Reddish-violet liquid
CAS-Number	339527-79-6
Molecular Formula	C25H26N4O3
Molecular Weight	430.51
Identity	1H-NMR
Solvents	DMSO
Melting Point	219°C
Boiling Point	219°C
Smiles	CN(C)C1=CC2=[O+]C(C=C(N(C)C)C=C3)=C3C(C4=CC=C(NC)C(N)=C4C([O-])=O)=C2C=C1
InChi Key	LNDSYTUQQCXPAM-UHFFFAOYSA-N
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at -20°C.
Hazard statements	H315-H319-H335
Precautionary statements	P261-P305 + P351 + P338
GHS Symbol	GHS07
Signal word	Warning
Transportation	Not dangerous goods
References	(1) H. Kojima, et al., Anal. Chem.73, 1967 (2001), (2) H. Kojima & N. Nagano, Advanced Materials 12, 763 (2000), (3) H. Kojima, et al., Tetrahedr. Lett. 41, 69 (2000), (4) Z. Lacza, et al., J. Pharmacol. Toxicol. Meth. 52, 335 (2005)
InChi	InChI=1S/C25H26N4O3/c1-27-19-11-10-18(23(24(19)26)25(30)31)22-16-8-6-14(28(2)3)12-20(16)32-21-13-15(29(4)5)7-9-17(21)22/h6-13,27H,26H2,1-5H3
Quantity	1 mg, Bulk