Specifications
- Purity
- ≥98% (HPLC)
- Identity
- 1H-NMR
- Appearance
- Light brown powder
Properties
- Fluorescence
- λex 571 nm, λem 585 nm in DMSO
- Solvents
- DMSO, DMF
Available from stock
10-Acetyl-3,7-dihydroxyphenoxazine
- SKU
- A0022
Category: Fluorescent Detection
- Synonyms
- Amplex® Red (Registered trademark of LifeTechnologies)
- 119171-73-2
- CAS-Number
- C14H11NO4
- Molecular Formula
- 257.24
- Molecular Weight
- Long Term Storage
- -20°C
- Use / Stability
- Stable for at least 2 years after receipt when stored at -20°C.
- Handling Advice
- Protect from light and moisture.
- Short Term Storage
- +4°C
- Shipping
- AMBIENT
- Transportation
- Not dangerous goods
- Signal word
- Warning
- GHS Symbol
- GHS07
- Hazard statements
- H302
- Description
- 10-acetyl-3,7-dihydroxyphenoxazin (Amplex Red) is a non-fluorescent, highly sensitive and stable probe for H2O2. Amplex Red is an applicable as a fluorogenic substrate for peroxidase. In the presence of horseradish peroxidase (HRP), the Amplex Red reagent reacts in a 1:1 stechiometry with H2O2 to produce highly fluorescent resorufin. Because H2O2 is produced in many different enzymatic reactions, the Amplex Red reagent allows researchers to detect the activity of many different enzymes. 10-acetyl-3,7-dihydroxyphenoxazin has absorption and fluorescence emission maxima of approximately 563 nm and 587 nm, respectively, and because its extinction coefficient is high (54,000 cm-1M-1), the assay can be performed either fluorometrically or spectrophotometrically.
- InChi
- InChI=1S/C14H11NO4/c1-8(16)15-11-4-2-9(17)6-13(11)19-14-7-10(18)3-5-12(14)15/h2-7,17-18H,1H3
- References
- (1) I. Snyrychova et al.: Phys. Plantarum, 135(1), 1-18 (2009) , (2) J.G. Montay et al.: J. Immunol. Meth. 202, 133 (1997) , (3) M. Zhou et al.: Anal. Biochem. 253, 162 (1997)
- InChi Key
- PKYCWFICOKSIHZ-UHFFFAOYSA-N
- Smiles
- CC(=O)N1C2=CC=C(O)C=C2OC2=C1C=CC(O)=C2
10-acetyl-3,7-dihydroxyphenoxazin (Amplex Red) is a non-fluorescent, highly sensitive and stable probe for H2O2. Amplex Red is an applicable as a fluorogenic substrate for peroxidase. In the presence of horseradish peroxidase (HRP), the Amplex Red reagent reacts in a 1:1 stechiometry with H2O2 to produce highly fluorescent resorufin. Because H2O2 is produced in many different enzymatic reactions, the Amplex Red reagent allows researchers to detect the activity of many different enzymes. 10-acetyl-3,7-dihydroxyphenoxazin has absorption and fluorescence emission maxima of approximately 563 nm and 587 nm, respectively, and because its extinction coefficient is high (54,000 cm-1M-1), the assay can be performed either fluorometrically or spectrophotometrically.
Additional information
| Synonyms | Amplex® Red (Registered trademark of LifeTechnologies) |
|---|---|
| Purity | ≥98% (HPLC) |
| Appearance | Light brown powder |
| CAS-Number | 119171-73-2 |
| Molecular Formula | C14H11NO4 |
| Molecular Weight | 257.24 |
| Identity | 1H-NMR |
| Solvents | DMSO, DMF |
| Fluorescence | λex 571 nm, λem 585 nm in DMSO |
| Smiles | CC(=O)N1C2=CC=C(O)C=C2OC2=C1C=CC(O)=C2 |
| InChi Key | PKYCWFICOKSIHZ-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Hazard statements | H302 |
| GHS Symbol | GHS07 |
| Signal word | Warning |
| Transportation | Not dangerous goods |
| References | (1) I. Snyrychova et al.: Phys. Plantarum, 135(1), 1-18 (2009), (2) J.G. Montay et al.: J. Immunol. Meth. 202, 133 (1997), (3) M. Zhou et al.: Anal. Biochem. 253, 162 (1997) |
| InChi | InChI=1S/C14H11NO4/c1-8(16)15-11-4-2-9(17)6-13(11)19-14-7-10(18)3-5-12(14)15/h2-7,17-18H,1H3 |
| Quantity | 25 mg, 200 mg, Bulk |
