- Synonyms: Amplex® Red (Registered trademark of LifeTechnologies)
- Purity: ≥98% (HPLC)
- Appearance: Light brown powder.
- CAS-Number: 119171-73-2
- Molecular Formula: C14H11NO4
- Molecular Weight: 257.24
- Identity: 1H-NMR
- Solvents: DMSO or DMF
- Fluorescence: λex 571 nm; λem 585 nm in DMSO
- Smiles: CC(=O)N1C2=CC=C(O)C=C2OC2=C1C=CC(O)=C2
- InChi: InChI=1S/C14H11NO4/c1-8(16)15-11-4-2-9(17)6-13(11)19-14-7-10(18)3-5-12(14)15/h2-7,17-18H,1H3
- InChi Key: PKYCWFICOKSIHZ-UHFFFAOYSA-N
Available from stock
10-Acetyl-3,7-dihydroxyphenoxazine
SKU: A0022
Category: Fluorescent Detection
- Shipping: AMBIENT
- Short Term Storage: +4°C
- Long Term Storage: -20°C
- Handling Advice: Protect from light and moisture.
- Use / Stability: Stable for at least 2 years after receipt when stored at -20°C.
- Description: 10-acetyl-3,7-dihydroxyphenoxazin (Amplex Red) is a non-fluorescent, highly sensitive and stable probe for H2O2. Amplex Red is an applicable as a fluorogenic substrate for peroxidase. In the presence of horseradish peroxidase (HRP), the Amplex Red reagent reacts in a 1:1 stechiometry with H2O2 to produce highly fluorescent resorufin. Because H2O2 is produced in many different enzymatic reactions, the Amplex Red reagent allows researchers to detect the activity of many different enzymes. 10-acetyl-3,7-dihydroxyphenoxazin has absorption and fluorescence emission maxima of approximately 563 nm and 587 nm, respectively, and because its extinction coefficient is high (54,000 cm-1M-1), the assay can be performed either fluorometrically or spectrophotometrically.
- References: (1) I. Snyrychova et al.; Phys. Plantarum, 135(1), 1-18 (2009), (2) J.G. Montay et al.; J. Immunol. Meth. 202, 133 (1997), (3) M. Zhou et al.; Anal. Biochem. 253, 162 (1997)
10-acetyl-3,7-dihydroxyphenoxazin (Amplex Red) is a non-fluorescent, highly sensitive and stable probe for H2O2. Amplex Red is an applicable as a fluorogenic substrate for peroxidase. In the presence of horseradish peroxidase (HRP), the Amplex Red reagent reacts in a 1:1 stechiometry with H2O2 to produce highly fluorescent resorufin. Because H2O2 is produced in many different enzymatic reactions, the Amplex Red reagent allows researchers to detect the activity of many different enzymes. 10-acetyl-3,7-dihydroxyphenoxazin has absorption and fluorescence emission maxima of approximately 563 nm and 587 nm, respectively, and because its extinction coefficient is high (54,000 cm-1M-1), the assay can be performed either fluorometrically or spectrophotometrically.
Additional information
| Identity | 1H-NMR |
|---|---|
| Handling Advice | Protect from light and moisture. |
| Availability | 1 |
| CAS-Number | 119171-73-2 |
| Long Term Storage | -20°C |
| InChi Key | PKYCWFICOKSIHZ-UHFFFAOYSA-N |
| Quantity | 200 mg, 25 mg, Bulk |
| Appearance | Light brown powder. |
| InChi | InChI=1S/C14H11NO4/c1-8(16)15-11-4-2-9(17)6-13(11)19-14-7-10(18)3-5-12(14)15/h2-7,17-18H,1H3 |
| Fluorescence | λex 571 nm; λem 585 nm in DMSO |
| Molecular Formula | |
| Short Term Storage | +4°C |
| Purity | |
| Pricetable | |
| References | (1) I. Snyrychova et al.; Phys. Plantarum, 135(1), 1-18 (2009), (2) J.G. Montay et al.; J. Immunol. Meth. 202, 133 (1997), (3) M. Zhou et al.; Anal. Biochem. 253, 162 (1997) |
| Smiles | CC(=O)N1C2=CC=C(O)C=C2OC2=C1C=CC(O)=C2 |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Molecular Weight | |
| Solvents | DMSO or DMF |
| Synonyms | Amplex® Red (Registered trademark of LifeTechnologies) |
| Shipping | AMBIENT |
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