10-Acetyl-3

Available from stock

10-Acetyl-3,7-dihydroxyphenoxazine

SKU
A0022

Categories: ,

Synonyms
Amplex® Red (Registered trademark of LifeTechnologies)
119171-73-2
CAS-Number
C14H11NO4
Molecular Formula
257.24
Molecular Weight

Specifications

Purity
≥98% (HPLC)
Identity
1H-NMR
Appearance
Light brown powder

Properties

Fluorescence
λex 571 nm, λem 585 nm in DMSO
Solvents
DMSO, DMF

Downloads

Safety Data Sheet
CDX A0022 MSDS.pdf
Long Term Storage
-20°C
Use / Stability
Stable for at least 2 years after receipt when stored at -20°C.
Handling Advice
Protect from light and moisture.
Short Term Storage
+4°C
Shipping
AMBIENT
Transportation
Not dangerous goods
Signal word
Warning
GHS Symbol
GHS07
Hazard statements
H302
Description
10-acetyl-3,7-dihydroxyphenoxazin (Amplex Red) is a non-fluorescent, highly sensitive and stable probe for H2O2. Amplex Red is an applicable as a fluorogenic substrate for peroxidase. In the presence of horseradish peroxidase (HRP), the Amplex Red reagent reacts in a 1:1 stechiometry with H2O2 to produce highly fluorescent resorufin. Because H2O2 is produced in many different enzymatic reactions, the Amplex Red reagent allows researchers to detect the activity of many different enzymes. 10-acetyl-3,7-dihydroxyphenoxazin has absorption and fluorescence emission maxima of approximately 563 nm and 587 nm, respectively, and because its extinction coefficient is high (54,000 cm-1M-1), the assay can be performed either fluorometrically or spectrophotometrically.
InChi
InChI=1S/C14H11NO4/c1-8(16)15-11-4-2-9(17)6-13(11)19-14-7-10(18)3-5-12(14)15/h2-7,17-18H,1H3
References
(1) I. Snyrychova et al.: Phys. Plantarum, 135(1), 1-18 (2009) , (2) J.G. Montay et al.: J. Immunol. Meth. 202, 133 (1997) , (3) M. Zhou et al.: Anal. Biochem. 253, 162 (1997)
InChi Key
PKYCWFICOKSIHZ-UHFFFAOYSA-N
Smiles
CC(=O)N1C2=CC=C(O)C=C2OC2=C1C=CC(O)=C2

10-acetyl-3,7-dihydroxyphenoxazin (Amplex Red) is a non-fluorescent, highly sensitive and stable probe for H2O2. Amplex Red is an applicable as a fluorogenic substrate for peroxidase. In the presence of horseradish peroxidase (HRP), the Amplex Red reagent reacts in a 1:1 stechiometry with H2O2 to produce highly fluorescent resorufin. Because H2O2 is produced in many different enzymatic reactions, the Amplex Red reagent allows researchers to detect the activity of many different enzymes. 10-acetyl-3,7-dihydroxyphenoxazin has absorption and fluorescence emission maxima of approximately 563 nm and 587 nm, respectively, and because its extinction coefficient is high (54,000 cm-1M-1), the assay can be performed either fluorometrically or spectrophotometrically.

SKU: A0022 Categories: ,

Additional information

Synonyms

Amplex® Red (Registered trademark of LifeTechnologies)
Purity

≥98% (HPLC)
Appearance

Light brown powder
CAS-Number

119171-73-2
Molecular Formula

C14H11NO4
Molecular Weight

257.24
Identity

1H-NMR
Solvents

DMSO, DMF
Fluorescence

λex 571 nm, λem 585 nm in DMSO
Smiles

CC(=O)N1C2=CC=C(O)C=C2OC2=C1C=CC(O)=C2
InChi Key

PKYCWFICOKSIHZ-UHFFFAOYSA-N
Shipping

AMBIENT
Short Term Storage

+4°C
Long Term Storage

-20°C
Handling Advice

Protect from light and moisture.
Use / Stability

Stable for at least 2 years after receipt when stored at -20°C.
Hazard statements

H302
GHS Symbol

GHS07
Signal word

Warning
Transportation

Not dangerous goods
References

(1) I. Snyrychova et al.: Phys. Plantarum, 135(1), 1-18 (2009), (2) J.G. Montay et al.: J. Immunol. Meth. 202, 133 (1997), (3) M. Zhou et al.: Anal. Biochem. 253, 162 (1997)
InChi

InChI=1S/C14H11NO4/c1-8(16)15-11-4-2-9(17)6-13(11)19-14-7-10(18)3-5-12(14)15/h2-7,17-18H,1H3
Quantity

25 mg, 200 mg, Bulk

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