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    10-Acetyl-3

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    10-Acetyl-3,7-dihydroxyphenoxazine

    SKU
    A0022

    Category: Fluorescent Detection

    Synonyms
    Amplex® Red (Registered trademark of LifeTechnologies)
    119171-73-2
    CAS-Number
    C14H11NO4
    Molecular Formula
    257.24
    Molecular Weight

    • Product Data
    • Handling
    • Safety Information
    • Details

    Specifications

    Purity
    ≥98% (HPLC)
    Identity
    1H-NMR
    Appearance
    Light brown powder

    Properties

    Fluorescence
    λex 571 nm, λem 585 nm in DMSO
    Solvents
    DMSO, DMF

    Downloads

    Safety Data Sheet
    CDX A0022 MSDS.pdf
    Long Term Storage
    -20°C
    Use / Stability
    Stable for at least 2 years after receipt when stored at -20°C.
    Handling Advice
    Protect from light and moisture.
    Short Term Storage
    +4°C
    Shipping
    AMBIENT
    Transportation
    Not dangerous goods
    Signal word
    Warning
    GHS Symbol
    GHS07
    Hazard statements
    H302
    Description
    10-acetyl-3,7-dihydroxyphenoxazin (Amplex Red) is a non-fluorescent, highly sensitive and stable probe for H2O2. Amplex Red is an applicable as a fluorogenic substrate for peroxidase. In the presence of horseradish peroxidase (HRP), the Amplex Red reagent reacts in a 1:1 stechiometry with H2O2 to produce highly fluorescent resorufin. Because H2O2 is produced in many different enzymatic reactions, the Amplex Red reagent allows researchers to detect the activity of many different enzymes. 10-acetyl-3,7-dihydroxyphenoxazin has absorption and fluorescence emission maxima of approximately 563 nm and 587 nm, respectively, and because its extinction coefficient is high (54,000 cm-1M-1), the assay can be performed either fluorometrically or spectrophotometrically.
    InChi
    InChI=1S/C14H11NO4/c1-8(16)15-11-4-2-9(17)6-13(11)19-14-7-10(18)3-5-12(14)15/h2-7,17-18H,1H3
    References
    (1) I. Snyrychova et al.: Phys. Plantarum, 135(1), 1-18 (2009) , (2) J.G. Montay et al.: J. Immunol. Meth. 202, 133 (1997) , (3) M. Zhou et al.: Anal. Biochem. 253, 162 (1997)
    InChi Key
    PKYCWFICOKSIHZ-UHFFFAOYSA-N
    Smiles
    CC(=O)N1C2=CC=C(O)C=C2OC2=C1C=CC(O)=C2

    10-acetyl-3,7-dihydroxyphenoxazin (Amplex Red) is a non-fluorescent, highly sensitive and stable probe for H2O2. Amplex Red is an applicable as a fluorogenic substrate for peroxidase. In the presence of horseradish peroxidase (HRP), the Amplex Red reagent reacts in a 1:1 stechiometry with H2O2 to produce highly fluorescent resorufin. Because H2O2 is produced in many different enzymatic reactions, the Amplex Red reagent allows researchers to detect the activity of many different enzymes. 10-acetyl-3,7-dihydroxyphenoxazin has absorption and fluorescence emission maxima of approximately 563 nm and 587 nm, respectively, and because its extinction coefficient is high (54,000 cm-1M-1), the assay can be performed either fluorometrically or spectrophotometrically.

    SKU: A0022 Category: Fluorescent Detection
    • Additional information

    Additional information

    Synonyms

    Amplex® Red (Registered trademark of LifeTechnologies)

    Purity

    ≥98% (HPLC)

    Appearance

    Light brown powder

    CAS-Number

    119171-73-2

    Molecular Formula

    C14H11NO4

    Molecular Weight

    257.24

    Identity

    1H-NMR

    Solvents

    DMSO, DMF

    Fluorescence

    λex 571 nm, λem 585 nm in DMSO

    Smiles

    CC(=O)N1C2=CC=C(O)C=C2OC2=C1C=CC(O)=C2

    InChi Key

    PKYCWFICOKSIHZ-UHFFFAOYSA-N

    Shipping

    AMBIENT

    Short Term Storage

    +4°C

    Long Term Storage

    -20°C

    Handling Advice

    Protect from light and moisture.

    Use / Stability

    Stable for at least 2 years after receipt when stored at -20°C.

    Hazard statements

    H302

    GHS Symbol

    GHS07

    Signal word

    Warning

    Transportation

    Not dangerous goods

    References

    (1) I. Snyrychova et al.: Phys. Plantarum, 135(1), 1-18 (2009), (2) J.G. Montay et al.: J. Immunol. Meth. 202, 133 (1997), (3) M. Zhou et al.: Anal. Biochem. 253, 162 (1997)

    InChi

    InChI=1S/C14H11NO4/c1-8(16)15-11-4-2-9(17)6-13(11)19-14-7-10(18)3-5-12(14)15/h2-7,17-18H,1H3

    Quantity

    25 mg, 200 mg, Bulk

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