Specifications
- Purity
- ≥98% (HPLC)
- Appearance
- Yellow powder
- Identity
- 1H-NMR
Properties
- Solvents
- chloroform, DMSO
Documentation
- Safety Data Sheet (SDS)
- CDX C0036 MSDS.pdf
Available from stock
3-Carboxy-6,8-difluoro-7-hydroxycoumarin
- SKU
- C0036
Categories: Fluorescent Detection, Fluorescent Detection
- Synonyms
- NHS Pacific Blue succinimidyl ester , 2,5-Dioxopyrrolidin-1-yl 6,8-difluoro-7-hydroxy-2-oxo-2H-chromene-3-carboxylate
- 215868-33-0
- CAS-Number
- C14H7F2NO7
- Molecular Formula
- 339.2
- Molecular Weight
- Shipping
- AMBIENT
- Short Term Storage
- +4°C
- Long Term Storage
- -20°C
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at -20°C.
- Hazard statements
- H315-H319-H335
- Precautionary statements
- P261-P305 + P351 + P338
- GHS Symbol
- GHS07
- Signal word
- Warning
- Transportation
- Not dangerous goods
- Description
- This amine-reactive reagent can be used to create blue-fluorescent bioconjugates with excitation/emission maxima ~410/455 nm that are excitable by the 405 nm spectral line of the blue diode (violet) laser. Used in highly sensitive analyses of amines, amino acids and dipeptides. Amine-reactive reagents react with non-protonated aliphatic amine groups, including the amine terminus of proteins and the ?-amino group of lysines.
- Smiles
- OC1=C(F)C2=C(C=C1F)C=C(C(=O)ON1C(=O)CCC1=O)C(=O)O2
- InChi Key
- NZYXABPVYJRICY-UHFFFAOYSA-N
- References
- (1) B. Abrams, et al.: Anal. Biochem. 386, 262 (2009) , (2) T.N. Chiesl, et al.: Anal. Chem. 81, 2537 (2009) , (3) J.D. Cohen, et al.: Biochem. 50, 8221 (2011) , (4) A.M. Stockton, et al.: Dyes & Pigments 96, 148 (2013)
- InChi
- InChI=1S/C14H7F2NO7/c15-7-4-5-3-6(13(21)23-12(5)10(16)11(7)20)14(22)24-17-8(18)1-2-9(17)19/h3-4,20H,1-2H2
This amine-reactive reagent can be used to create blue-fluorescent bioconjugates with excitation/emission maxima ~410/455 nm that are excitable by the 405 nm spectral line of the blue diode (violet) laser. Used in highly sensitive analyses of amines, amino acids and dipeptides. Amine-reactive reagents react with non-protonated aliphatic amine groups, including the amine terminus of proteins and the ?-amino group of lysines.
Additional information
| Synonyms | NHS Pacific Blue succinimidyl ester, 2,5-Dioxopyrrolidin-1-yl 6,8-difluoro-7-hydroxy-2-oxo-2H-chromene-3-carboxylate |
|---|---|
| Purity | ≥98% (HPLC) |
| Appearance | Yellow powder |
| CAS-Number | 215868-33-0 |
| Molecular Formula | C14H7F2NO7 |
| Molecular Weight | 339.2 |
| Identity | 1H-NMR |
| Solvents | chloroform, DMSO |
| Smiles | OC1=C(F)C2=C(C=C1F)C=C(C(=O)ON1C(=O)CCC1=O)C(=O)O2 |
| InChi Key | NZYXABPVYJRICY-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Hazard statements | H315-H319-H335 |
| Precautionary statements | P261-P305 + P351 + P338 |
| GHS Symbol | GHS07 |
| Signal word | Warning |
| Transportation | Not dangerous goods |
| References | (1) B. Abrams, et al.: Anal. Biochem. 386, 262 (2009), (2) T.N. Chiesl, et al.: Anal. Chem. 81, 2537 (2009), (3) J.D. Cohen, et al.: Biochem. 50, 8221 (2011), (4) A.M. Stockton, et al.: Dyes & Pigments 96, 148 (2013) |
| InChi | InChI=1S/C14H7F2NO7/c15-7-4-5-3-6(13(21)23-12(5)10(16)11(7)20)14(22)24-17-8(18)1-2-9(17)19/h3-4,20H,1-2H2 |
| Quantity | 25 mg, 100 mg, Bulk |
