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    3-Carboxy-6

    Available from stock

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      3-Carboxy-6,8-difluoro-7-hydroxycoumarin

      SKU
      C0036

      Categories: Fluorescent Detection, Fluorescent Detection

      Synonyms
      NHS Pacific Blue succinimidyl ester , 2,5-Dioxopyrrolidin-1-yl 6,8-difluoro-7-hydroxy-2-oxo-2H-chromene-3-carboxylate
      215868-33-0
      CAS-Number
      C14H7F2NO7
      Molecular Formula
      339.2
      Molecular Weight

      For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

      • Product Data
      • Handling
      • Safety Information
      • Details

      Specifications

      Purity
      ≥98% (HPLC)
      Appearance
      Yellow powder
      Identity
      1H-NMR

      Properties

      Solvents
      chloroform, DMSO

      Documentation

      Safety Data Sheet (SDS)
      CDX C0036 MSDS.pdf
      Shipping
      AMBIENT
      Short Term Storage
      +4°C
      Long Term Storage
      -20°C
      Handling Advice
      Protect from light and moisture.
      Use / Stability
      Stable for at least 2 years after receipt when stored at -20°C.
      Hazard statements
      H315-H319-H335
      Precautionary statements
      P261-P305 + P351 + P338
      GHS Symbol
      GHS07
      Signal word
      Warning
      Transportation
      Not dangerous goods
      Description
      This amine-reactive reagent can be used to create blue-fluorescent bioconjugates with excitation/emission maxima ~410/455 nm that are excitable by the 405 nm spectral line of the blue diode (violet) laser. Used in highly sensitive analyses of amines, amino acids and dipeptides. Amine-reactive reagents react with non-protonated aliphatic amine groups, including the amine terminus of proteins and the ?-amino group of lysines.
      Smiles
      OC1=C(F)C2=C(C=C1F)C=C(C(=O)ON1C(=O)CCC1=O)C(=O)O2
      InChi Key
      NZYXABPVYJRICY-UHFFFAOYSA-N
      References
      (1) B. Abrams, et al.: Anal. Biochem. 386, 262 (2009) , (2) T.N. Chiesl, et al.: Anal. Chem. 81, 2537 (2009) , (3) J.D. Cohen, et al.: Biochem. 50, 8221 (2011) , (4) A.M. Stockton, et al.: Dyes & Pigments 96, 148 (2013)
      InChi
      InChI=1S/C14H7F2NO7/c15-7-4-5-3-6(13(21)23-12(5)10(16)11(7)20)14(22)24-17-8(18)1-2-9(17)19/h3-4,20H,1-2H2

      This amine-reactive reagent can be used to create blue-fluorescent bioconjugates with excitation/emission maxima ~410/455 nm that are excitable by the 405 nm spectral line of the blue diode (violet) laser. Used in highly sensitive analyses of amines, amino acids and dipeptides. Amine-reactive reagents react with non-protonated aliphatic amine groups, including the amine terminus of proteins and the ?-amino group of lysines.

      SKU: C0036 Categories: Fluorescent Detection, Fluorescent Detection
      • Additional information

      Additional information

      Synonyms

      NHS Pacific Blue succinimidyl ester, 2,5-Dioxopyrrolidin-1-yl 6,8-difluoro-7-hydroxy-2-oxo-2H-chromene-3-carboxylate

      Purity

      ≥98% (HPLC)

      Appearance

      Yellow powder

      CAS-Number

      215868-33-0

      Molecular Formula

      C14H7F2NO7

      Molecular Weight

      339.2

      Identity

      1H-NMR

      Solvents

      chloroform, DMSO

      Smiles

      OC1=C(F)C2=C(C=C1F)C=C(C(=O)ON1C(=O)CCC1=O)C(=O)O2

      InChi Key

      NZYXABPVYJRICY-UHFFFAOYSA-N

      Shipping

      AMBIENT

      Short Term Storage

      +4°C

      Long Term Storage

      -20°C

      Handling Advice

      Protect from light and moisture.

      Use / Stability

      Stable for at least 2 years after receipt when stored at -20°C.

      Hazard statements

      H315-H319-H335

      Precautionary statements

      P261-P305 + P351 + P338

      GHS Symbol

      GHS07

      Signal word

      Warning

      Transportation

      Not dangerous goods

      References

      (1) B. Abrams, et al.: Anal. Biochem. 386, 262 (2009), (2) T.N. Chiesl, et al.: Anal. Chem. 81, 2537 (2009), (3) J.D. Cohen, et al.: Biochem. 50, 8221 (2011), (4) A.M. Stockton, et al.: Dyes & Pigments 96, 148 (2013)

      InChi

      InChI=1S/C14H7F2NO7/c15-7-4-5-3-6(13(21)23-12(5)10(16)11(7)20)14(22)24-17-8(18)1-2-9(17)19/h3-4,20H,1-2H2

      Quantity

      25 mg, 100 mg, Bulk

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