Specifications
- Purity
- ≥98% (Titration)
- Appearance
- Dark red crystals or powder
- Identity
- 1H-NMR
Properties
- Solvents
- Soluble in DMSO or methanol.
- Melting Point
- 278 °C (dec.) (lit.)
Available from stock
3,3′-Diethyloxacarbocyanine iodide
- SKU
- D0180
Categories: Fluorescent Detection, Fluorescent Detection
- Synonyms
- DiOC2(3), 3-Ethyl-2-[3-(3-ethyl-2(3H)-benzoxazolylidene)-1-propenyl]benzoxazolium iodide, DOC iodide
- 905-96-4
- CAS-Number
- C21H19IN2S
- Molecular Formula
- 460.31
- Molecular Weight
- Shipping
- AMBIENT
- Short Term Storage
- RT
- Long Term Storage
- RT
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at RT.
- Transportation
- Excepted Quantities
- Description
- Dansyl fluoride is a fluorogenic reagent used as a specific fluorescent probe for serine proteases and is a serine-type protease inhibitor. It is an active site directed fluorescent inhibitor of guanidinobenzoatase (GB), which binds to both the colonic isoenzymic forms of the GB. The GB on cell surfaces after binding dansyl fluoride makes the cells fluoresce blue. It has been used for the location of tumour cells in frozen sections of human lung squamous cell carcinoma and colonic carcinoma tissues. It is used as a precursor for the synthesis of other derivatives like dansyl amide. Spectral data: λex 360nm; λem 580nm (methanol).
- Smiles
- CCN1/C(OC2=CC=CC=C12)=C\C=C\C3=[N+](CC)C4=CC=CC=C4O3.[I-]
- InChi Key
- FIZZUEJIOKEFFZ-UHFFFAOYSA-M
- References
- (1) W.L Vaz & G. Schoellmann; Biochim. Biophys. Acta 439, 194 (1976) , (2) J. Stackhouse & F.H. Westheimer; J. Org. Chem. 46, 1891 (1981) , (3) G. Schoellmann, et al.; Biochim. Biophys. Acta 704, 403 (1982) , (4) R.F. Chen & C.H. Scott; Anal. Lett. 18, 393 (1985) , (5) M. Anees & E.W. Benbow; J. Enzyme Inhib. 10, 195 (1996) , (6) R. Zhou, et al.; PNAS 95, 4959 (1998)
- InChi
- (1) J.V. Small et al. J. Cell Sci. 89, 21, (1988)
Dansyl fluoride is a fluorogenic reagent used as a specific fluorescent probe for serine proteases and is a serine-type protease inhibitor. It is an active site directed fluorescent inhibitor of guanidinobenzoatase (GB), which binds to both the colonic isoenzymic forms of the GB. The GB on cell surfaces after binding dansyl fluoride makes the cells fluoresce blue. It has been used for the location of tumour cells in frozen sections of human lung squamous cell carcinoma and colonic carcinoma tissues. It is used as a precursor for the synthesis of other derivatives like dansyl amide. Spectral data: λex 360nm; λem 580nm (methanol).
Additional information
| Synonyms | DiOC2(3), 3-Ethyl-2-[3-(3-ethyl-2(3H)-benzoxazolylidene)-1-propenyl]benzoxazolium iodide, DOC iodide |
|---|---|
| Purity | ≥98% (Titration) |
| Appearance | Dark red crystals or powder |
| CAS-Number | 905-96-4 |
| Molecular Formula | C21H19IN2S |
| Molecular Weight | 460.31 |
| Identity | 1H-NMR |
| Solvents | Soluble in DMSO or methanol. |
| Melting Point | 278 °C (dec.) (lit.) |
| Smiles | CCN1/C(OC2=CC=CC=C12)=C\C=C\C3=[N+](CC)C4=CC=CC=C4O3.[I-] |
| InChi Key | FIZZUEJIOKEFFZ-UHFFFAOYSA-M |
| Shipping | AMBIENT |
| Short Term Storage | RT |
| Long Term Storage | RT |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at RT. |
| Transportation | Excepted Quantities |
| References | (1) W.L Vaz & G. Schoellmann; Biochim. Biophys. Acta 439, 194 (1976), (2) J. Stackhouse & F.H. Westheimer; J. Org. Chem. 46, 1891 (1981), (3) G. Schoellmann, et al.; Biochim. Biophys. Acta 704, 403 (1982), (4) R.F. Chen & C.H. Scott; Anal. Lett. 18, 393 (1985), (5) M. Anees & E.W. Benbow; J. Enzyme Inhib. 10, 195 (1996), (6) R. Zhou, et al.; PNAS 95, 4959 (1998) |
| InChi | (1) J.V. Small et al. J. Cell Sci. 89, 21, (1988) |
| Quantity | 1 g, Bulk |
