Available from stock
Categories: Fluorescent Detection, Fluorescent Detection
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
5(6)-Carboxyfluorescein diacetate N-succinimidyl ester is a useful fluorescent tracer that can passivley diffuse into cells and covalently label intracellular proteins, resulting in long-term cell labeling. The reagent itself is colorless and nonfluorescent but becomes brightly green fluorescent once it is hydrolyzed by intracellular esterases.
| Synonyms | 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester, CFSE |
|---|---|
| Purity | ≥90% (HPLC) |
| Appearance | White or slightly yellow powder |
| CAS-Number | 150347-59-4 |
| Molecular Formula | C29H19NO11 |
| Molecular Weight | 557.46 |
| Identity | 1H-NMR |
| Solvents | DMSO, DMF, chloroform |
| Melting Point | 152-154 °C (lit.) |
| Fluorescence | λex 492 nm, λem 517 nm in 0.1 M Tris pH 8.0 |
| Smiles | CC(=O)ON1C(=O)CCC1=O.CC(=O)OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=CC=C3)C1=CC=C(OC(C)=O)C=C1O2 |
| InChi Key | KQPBJOQXHVRGEG-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Transportation | Not dangerous goods |
| References | (1) V.V. Ganusov et al.: J. Immun. Meth. 298(1-2), 183 (2005), (2) C. Bergsdorf et al.: FEBS Letters 536(1-3), 120 (2003), (3) I.E. Dumitriu et al.: Analyt. Biochem. 299, 247 (2001), (4) A. Lyons et al.: Meth. Cell Biol. 63, 375 (2001) |
| InChi | InChI=1S/C24H16O7.C6H7NO4/c1-13(25)28-15-7-9-19-21(11-15)30-22-12-16(29-14(2)26)8-10-20(22)24(19)18-6-4-3-5-17(18)23(27)31-24 |
| Quantity | 50 mg, 500 mg, Bulk |
5(6)-TAMRA
