- Synonyms: 5(6)-Carboxyfluorescein diacetate
- Purity: ≥90% (HPLC)
- Appearance: White powder
- CAS-Number: 124387-19-5
- Molecular Formula: C25H16O9
- Molecular Weight: 460.39
- Identity: 1H-NMR
- Solvents: DMSO
- Fluorescence: λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0, esterase
- Smiles: CC(O)=O.CC(=O)OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=CC=C3)C1=CC=C(OC(C)=O)C=C1O2
- InChi: InChI=1S/C20H12O5.C2H4O2/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20
- InChi Key: DCPDRLHHNBUQPJ-UHFFFAOYSA-N
Available from stock
5(6)-CFDA
SKU: C0046
Category: Fluorescent Detection
- Shipping: AMBIENT
- Short Term Storage: +4°C
- Long Term Storage: +4°C
- Handling Advice: Protect from light and moisture.
- Use / Stability: Stable for at least 2 years after receipt when stored at +4°C.
- Description: CFDA is a popular, sensitive, fluorescent substrate for measuring esterase activity in live cells. It is often used in cell viability assays and Live-Dead assay systems, especially automated cell viability assays. The cleavage product produced upon enzymatic or chemical hydrolysis of the acetate group(s) is carboxyfluorescein which is well-retained inside live cells, while the substrate CFDA is membrane permeant.
- References: (1) M. Bradley et al.; Bioorg. Med. Chem. Lett. 18(1), 313 (2008), (2) K. Hashizaki et al.; Chem. Pharm. Bull. 54(1), 80 (2006), (3) M.L. Graber et al.; Anal. Biochem. 156, 202 (1986), (4) D.F. Babcock et al.; J. Biol. Chem. 258, 6380 (1983)
CFDA is a popular, sensitive, fluorescent substrate for measuring esterase activity in live cells. It is often used in cell viability assays and Live-Dead assay systems, especially automated cell viability assays. The cleavage product produced upon enzymatic or chemical hydrolysis of the acetate group(s) is carboxyfluorescein which is well-retained inside live cells, while the substrate CFDA is membrane permeant.
Additional information
| Identity | 1H-NMR |
|---|---|
| Handling Advice | Protect from light and moisture. |
| Availability | 1 |
| CAS-Number | 124387-19-5 |
| Long Term Storage | +4°C |
| InChi Key | DCPDRLHHNBUQPJ-UHFFFAOYSA-N |
| Quantity | 1 g, 100 mg, 25 mg, Bulk |
| Appearance | White powder |
| InChi | InChI=1S/C20H12O5.C2H4O2/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20 |
| Fluorescence | λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0, esterase |
| Molecular Formula | |
| Short Term Storage | +4°C |
| Purity | |
| Pricetable | |
| References | (1) M. Bradley et al.; Bioorg. Med. Chem. Lett. 18(1), 313 (2008), (2) K. Hashizaki et al.; Chem. Pharm. Bull. 54(1), 80 (2006), (3) M.L. Graber et al.; Anal. Biochem. 156, 202 (1986), (4) D.F. Babcock et al.; J. Biol. Chem. 258, 6380 (1983) |
| Smiles | CC(O)=O.CC(=O)OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=CC=C3)C1=CC=C(OC(C)=O)C=C1O2 |
| Use / Stability | Stable for at least 2 years after receipt when stored at +4°C. |
| Molecular Weight | |
| Solvents | DMSO |
| Synonyms | 5(6)-Carboxyfluorescein diacetate |
| Shipping | AMBIENT |
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