5(6)-CFDA

SKU: C0046

Category: Fluorescent Detection

Synonyms: 5(6)-Carboxyfluorescein diacetate; 124387-19-5
CAS-Number: C₂₅H₁₆O₉
Molecular Formula: 460.39
Molecular Weight

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥90% (HPLC)
Identity: 1H-NMR
Appearance: White powder

Properties

Fluorescence: λex 492 nm, λem 517 nm in 0.1 M Tris pH 8.0, esterase
Solvents: DMSO

Short Term Storage: +4°C
Use / Stability: Stable for at least 2 years after receipt when stored at +4°C.
Handling Advice: Protect from light and moisture.
Long Term Storage: +4°C
Shipping: AMBIENT

Transportation: Not dangerous goods

Description: CFDA is a popular, sensitive, fluorescent substrate for measuring esterase activity in live cells. It is often used in cell viability assays and Live-Dead assay systems, especially automated cell viability assays. The cleavage product produced upon enzymatic or chemical hydrolysis of the acetate group(s) is carboxyfluorescein which is well-retained inside live cells, while the substrate CFDA is membrane permeant.
InChi: InChI=1S/C24H16O7.C2H4O2/c1-13(25)28-15-7-9-19-21(11-15)30-22-12-16(29-14(2)26)8-10-20(22)24(19)18-6-4-3-5-17(18)23(27)31-24
References: (1) C. Thrane et al.: J. Microbiol.l Meth. 28(1), 35 (1997) , (2) G. Liminga et al.: Anti-Cancer Drugs 6(4), 578 (1995) , (3) L.S. DeClerck et al.: J. Immun. Meth. 172(1), 115 (1994)
InChi Key: DCPDRLHHNBUQPJ-UHFFFAOYSA-N
Smiles: CC(O)=O.CC(=O)OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=CC=C3)C1=CC=C(OC(C)=O)C=C1O2

CFDA is a popular, sensitive, fluorescent substrate for measuring esterase activity in live cells. It is often used in cell viability assays and Live-Dead assay systems, especially automated cell viability assays. The cleavage product produced upon enzymatic or chemical hydrolysis of the acetate group(s) is carboxyfluorescein which is well-retained inside live cells, while the substrate CFDA is membrane permeant.

SKU: C0046 Category: Fluorescent Detection

Additional information

Additional information

Synonyms	5(6)-Carboxyfluorescein diacetate
Purity	≥90% (HPLC)
Appearance	White powder
CAS-Number	124387-19-5
Molecular Formula	C25H16O9
Molecular Weight	460.39
Identity	1H-NMR
Solvents	DMSO
Fluorescence	λex 492 nm, λem 517 nm in 0.1 M Tris pH 8.0, esterase
Smiles	CC(O)=O.CC(=O)OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=CC=C3)C1=CC=C(OC(C)=O)C=C1O2
InChi Key	DCPDRLHHNBUQPJ-UHFFFAOYSA-N
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	+4°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at +4°C.
Transportation	Not dangerous goods
References	(1) C. Thrane et al.: J. Microbiol.l Meth. 28(1), 35 (1997), (2) G. Liminga et al.: Anti-Cancer Drugs 6(4), 578 (1995), (3) L.S. DeClerck et al.: J. Immun. Meth. 172(1), 115 (1994)
InChi	InChI=1S/C24H16O7.C2H4O2/c1-13(25)28-15-7-9-19-21(11-15)30-22-12-16(29-14(2)26)8-10-20(22)24(19)18-6-4-3-5-17(18)23(27)31-24
Quantity	25 mg, 100 mg, 1 g, Bulk