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    5(6)-CFDA

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    5(6)-CFDA

    SKU
    C0046

    Category: Fluorescent Detection

    Synonyms
    5(6)-Carboxyfluorescein diacetate
    124387-19-5
    CAS-Number
    C25H16O9
    Molecular Formula
    460.39
    Molecular Weight

    • Product Data
    • Handling
    • Safety Information
    • Details

    Specifications

    Purity
    ≥90% (HPLC)
    Identity
    1H-NMR
    Appearance
    White powder

    Properties

    Fluorescence
    λex 492 nm, λem 517 nm in 0.1 M Tris pH 8.0, esterase
    Solvents
    DMSO
    Short Term Storage
    +4°C
    Use / Stability
    Stable for at least 2 years after receipt when stored at +4°C.
    Handling Advice
    Protect from light and moisture.
    Long Term Storage
    +4°C
    Shipping
    AMBIENT
    Transportation
    Not dangerous goods
    Description
    CFDA is a popular, sensitive, fluorescent substrate for measuring esterase activity in live cells. It is often used in cell viability assays and Live-Dead assay systems, especially automated cell viability assays. The cleavage product produced upon enzymatic or chemical hydrolysis of the acetate group(s) is carboxyfluorescein which is well-retained inside live cells, while the substrate CFDA is membrane permeant.
    InChi
    InChI=1S/C24H16O7.C2H4O2/c1-13(25)28-15-7-9-19-21(11-15)30-22-12-16(29-14(2)26)8-10-20(22)24(19)18-6-4-3-5-17(18)23(27)31-24
    References
    (1) C. Thrane et al.: J. Microbiol.l Meth. 28(1), 35 (1997) , (2) G. Liminga et al.: Anti-Cancer Drugs 6(4), 578 (1995) , (3) L.S. DeClerck et al.: J. Immun. Meth. 172(1), 115 (1994)
    InChi Key
    DCPDRLHHNBUQPJ-UHFFFAOYSA-N
    Smiles
    CC(O)=O.CC(=O)OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=CC=C3)C1=CC=C(OC(C)=O)C=C1O2

    CFDA is a popular, sensitive, fluorescent substrate for measuring esterase activity in live cells. It is often used in cell viability assays and Live-Dead assay systems, especially automated cell viability assays. The cleavage product produced upon enzymatic or chemical hydrolysis of the acetate group(s) is carboxyfluorescein which is well-retained inside live cells, while the substrate CFDA is membrane permeant.

    SKU: C0046 Category: Fluorescent Detection
    • Additional information

    Additional information

    Synonyms

    5(6)-Carboxyfluorescein diacetate

    Purity

    ≥90% (HPLC)

    Appearance

    White powder

    CAS-Number

    124387-19-5

    Molecular Formula

    C25H16O9

    Molecular Weight

    460.39

    Identity

    1H-NMR

    Solvents

    DMSO

    Fluorescence

    λex 492 nm, λem 517 nm in 0.1 M Tris pH 8.0, esterase

    Smiles

    CC(O)=O.CC(=O)OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=CC=C3)C1=CC=C(OC(C)=O)C=C1O2

    InChi Key

    DCPDRLHHNBUQPJ-UHFFFAOYSA-N

    Shipping

    AMBIENT

    Short Term Storage

    +4°C

    Long Term Storage

    +4°C

    Handling Advice

    Protect from light and moisture.

    Use / Stability

    Stable for at least 2 years after receipt when stored at +4°C.

    Transportation

    Not dangerous goods

    References

    (1) C. Thrane et al.: J. Microbiol.l Meth. 28(1), 35 (1997), (2) G. Liminga et al.: Anti-Cancer Drugs 6(4), 578 (1995), (3) L.S. DeClerck et al.: J. Immun. Meth. 172(1), 115 (1994)

    InChi

    InChI=1S/C24H16O7.C2H4O2/c1-13(25)28-15-7-9-19-21(11-15)30-22-12-16(29-14(2)26)8-10-20(22)24(19)18-6-4-3-5-17(18)23(27)31-24

    Quantity

    25 mg, 100 mg, 1 g, Bulk

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