Specifications
- Purity
- ≥90% (HPLC)
- Identity
- 1H-NMR
- Appearance
- White powder
Properties
- Fluorescence
- λex 492 nm, λem 517 nm in 0.1 M Tris pH 8.0, esterase
- Solvents
- DMSO
Category: Fluorescent Detection
CFDA is a popular, sensitive, fluorescent substrate for measuring esterase activity in live cells. It is often used in cell viability assays and Live-Dead assay systems, especially automated cell viability assays. The cleavage product produced upon enzymatic or chemical hydrolysis of the acetate group(s) is carboxyfluorescein which is well-retained inside live cells, while the substrate CFDA is membrane permeant.
Synonyms | 5(6)-Carboxyfluorescein diacetate |
---|---|
Purity | ≥90% (HPLC) |
Appearance | White powder |
CAS-Number | 124387-19-5 |
Molecular Formula | C25H16O9 |
Molecular Weight | 460.39 |
Identity | 1H-NMR |
Solvents | DMSO |
Fluorescence | λex 492 nm, λem 517 nm in 0.1 M Tris pH 8.0, esterase |
Smiles | CC(O)=O.CC(=O)OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=CC=C3)C1=CC=C(OC(C)=O)C=C1O2 |
InChi Key | DCPDRLHHNBUQPJ-UHFFFAOYSA-N |
Shipping | AMBIENT |
Short Term Storage | +4°C |
Long Term Storage | +4°C |
Handling Advice | Protect from light and moisture. |
Use / Stability | Stable for at least 2 years after receipt when stored at +4°C. |
Transportation | Not dangerous goods |
References | (1) C. Thrane et al.: J. Microbiol.l Meth. 28(1), 35 (1997), (2) G. Liminga et al.: Anti-Cancer Drugs 6(4), 578 (1995), (3) L.S. DeClerck et al.: J. Immun. Meth. 172(1), 115 (1994) |
InChi | InChI=1S/C24H16O7.C2H4O2/c1-13(25)28-15-7-9-19-21(11-15)30-22-12-16(29-14(2)26)8-10-20(22)24(19)18-6-4-3-5-17(18)23(27)31-24 |
Quantity | 25 mg, 100 mg, 1 g, Bulk |
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