Specifications
- Purity
- ≥85% (sum of isomers, HPCE)
- Identity
- 1H-NMR
- Appearance
- Dark red to brown powder
Properties
- Fluorescence
- λex 543 nm, λem 572 nm in methanol
- Melting Point
- ≥300 °C
- Solvents
- DMSO, DMF, methanol, aqueous buffers (pH ≥65)
Available from stock
5(6)-TAMRA
- SKU
- C0038
Category: Fluorescent Detection
- Synonyms
- 5(6)-Carboxytetramethylrhodamine , 150347-56-1
- 98181-63-6
- CAS-Number
- C25H22N2O5
- Molecular Formula
- 430.45
- Molecular Weight
- Long Term Storage
- +4°C
- Use / Stability
- Stable for at least 2 years after receipt when stored at +4°C.
- Handling Advice
- Protect from light and moisture.
- Short Term Storage
- +4°C
- Shipping
- AMBIENT
- Transportation
- Not dangerous goods
- Signal word
- Warning
- GHS Symbol
- GHS07
- Hazard statements
- H302
- Description
- Mixed isomers of carboxytetramethylrhodamine free acid. Tetramethylrhodamine (TMR) is one of the fluorophores most often used for preparing peptide, protein, nucleotide and nucleic acid conjugates. TMR dyes have been widely used as acceptors for FAM fluorophores in a variety of FRET studies. The dye can be coupled to primary or secondary amines via standard peptide chemistry.
- InChi
- InChI=1S/C24H22N2O3.C2H4O2/c1-25(2)15-9-11-19-21(13-15)28-22-14-16(26(3)4)10-12-20(22)24(19)18-8-6-5-7-17(18)23(27)29-24
- References
- (1) N.A. Evans et al.: J. Neurochem. 77, 476 (2001) , (2) K.L. Hess et al.: Cytometry 27, 145 (1997) , (3) W. Lutz et al.: J. Biol. Chem. 267, 1109 (1992)
- InChi Key
- VNRGODVGRCXKSJ-UHFFFAOYSA-N
- Smiles
- CC(O)=O.CN(C)C1=CC2=C(C=C1)C1(OC(=O)C3=C1C=CC=C3)C1=CC=C(C=C1O2)N(C)C
Mixed isomers of carboxytetramethylrhodamine free acid. Tetramethylrhodamine (TMR) is one of the fluorophores most often used for preparing peptide, protein, nucleotide and nucleic acid conjugates. TMR dyes have been widely used as acceptors for FAM fluorophores in a variety of FRET studies. The dye can be coupled to primary or secondary amines via standard peptide chemistry.
Additional information
| Synonyms | 5(6)-Carboxytetramethylrhodamine, 150347-56-1 |
|---|---|
| Purity | ≥85% (sum of isomers, HPCE) |
| Appearance | Dark red to brown powder |
| CAS-Number | 98181-63-6 |
| Molecular Formula | C25H22N2O5 |
| Molecular Weight | 430.45 |
| Identity | 1H-NMR |
| Solvents | DMSO, DMF, methanol, aqueous buffers (pH ≥65) |
| Melting Point | ≥300 °C |
| Fluorescence | λex 543 nm, λem 572 nm in methanol |
| Smiles | CC(O)=O.CN(C)C1=CC2=C(C=C1)C1(OC(=O)C3=C1C=CC=C3)C1=CC=C(C=C1O2)N(C)C |
| InChi Key | VNRGODVGRCXKSJ-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | +4°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at +4°C. |
| Hazard statements | H302 |
| GHS Symbol | GHS07 |
| Signal word | Warning |
| Transportation | Not dangerous goods |
| References | (1) N.A. Evans et al.: J. Neurochem. 77, 476 (2001), (2) K.L. Hess et al.: Cytometry 27, 145 (1997), (3) W. Lutz et al.: J. Biol. Chem. 267, 1109 (1992) |
| InChi | InChI=1S/C24H22N2O3.C2H4O2/c1-25(2)15-9-11-19-21(13-15)28-22-14-16(26(3)4)10-12-20(22)24(19)18-8-6-5-7-17(18)23(27)29-24 |
| Quantity | 100 mg, 2 g, Bulk |
