5(6)-TAMRA

SKU: C0038

Category: Fluorescent Detection

Synonyms: 5(6)-Carboxytetramethylrhodamine , 150347-56-1; 98181-63-6
CAS-Number: C₂₅H₂₂N₂O₅
Molecular Formula: 430.45
Molecular Weight

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥85% (sum of isomers, HPCE)
Identity: 1H-NMR
Appearance: Dark red to brown powder

Properties

Fluorescence: λex 543 nm, λem 572 nm in methanol
Melting Point: ≥300 °C
Solvents: DMSO, DMF, methanol, aqueous buffers (pH ≥65)

Long Term Storage: +4°C
Use / Stability: Stable for at least 2 years after receipt when stored at +4°C.
Handling Advice: Protect from light and moisture.
Short Term Storage: +4°C
Shipping: AMBIENT

Transportation: Not dangerous goods
Signal word: Warning
GHS Symbol: GHS07
Hazard statements: H302

Description: Mixed isomers of carboxytetramethylrhodamine free acid. Tetramethylrhodamine (TMR) is one of the fluorophores most often used for preparing peptide, protein, nucleotide and nucleic acid conjugates. TMR dyes have been widely used as acceptors for FAM fluorophores in a variety of FRET studies. The dye can be coupled to primary or secondary amines via standard peptide chemistry.
InChi: InChI=1S/C24H22N2O3.C2H4O2/c1-25(2)15-9-11-19-21(13-15)28-22-14-16(26(3)4)10-12-20(22)24(19)18-8-6-5-7-17(18)23(27)29-24
References: (1) N.A. Evans et al.: J. Neurochem. 77, 476 (2001) , (2) K.L. Hess et al.: Cytometry 27, 145 (1997) , (3) W. Lutz et al.: J. Biol. Chem. 267, 1109 (1992)
InChi Key: VNRGODVGRCXKSJ-UHFFFAOYSA-N
Smiles: CC(O)=O.CN(C)C1=CC2=C(C=C1)C1(OC(=O)C3=C1C=CC=C3)C1=CC=C(C=C1O2)N(C)C

Mixed isomers of carboxytetramethylrhodamine free acid. Tetramethylrhodamine (TMR) is one of the fluorophores most often used for preparing peptide, protein, nucleotide and nucleic acid conjugates. TMR dyes have been widely used as acceptors for FAM fluorophores in a variety of FRET studies. The dye can be coupled to primary or secondary amines via standard peptide chemistry.

SKU: C0038 Category: Fluorescent Detection

Additional information

Additional information

Synonyms	5(6)-Carboxytetramethylrhodamine, 150347-56-1
Purity	≥85% (sum of isomers, HPCE)
Appearance	Dark red to brown powder
CAS-Number	98181-63-6
Molecular Formula	C25H22N2O5
Molecular Weight	430.45
Identity	1H-NMR
Solvents	DMSO, DMF, methanol, aqueous buffers (pH ≥65)
Melting Point	≥300 °C
Fluorescence	λex 543 nm, λem 572 nm in methanol
Smiles	CC(O)=O.CN(C)C1=CC2=C(C=C1)C1(OC(=O)C3=C1C=CC=C3)C1=CC=C(C=C1O2)N(C)C
InChi Key	VNRGODVGRCXKSJ-UHFFFAOYSA-N
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	+4°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at +4°C.
Hazard statements	H302
GHS Symbol	GHS07
Signal word	Warning
Transportation	Not dangerous goods
References	(1) N.A. Evans et al.: J. Neurochem. 77, 476 (2001), (2) K.L. Hess et al.: Cytometry 27, 145 (1997), (3) W. Lutz et al.: J. Biol. Chem. 267, 1109 (1992)
InChi	InChI=1S/C24H22N2O3.C2H4O2/c1-25(2)15-9-11-19-21(13-15)28-22-14-16(26(3)4)10-12-20(22)24(19)18-8-6-5-7-17(18)23(27)29-24
Quantity	100 mg, 2 g, Bulk