Specifications
- Purity
- ≥97% (NMR)
- Appearance
- White to off-white powder
- Identity
- 1H-NMR
Properties
- Solvents
- DMSO, chloroform, water (insoluble)
Category: Fluorescent Detection, Fluorescent Detection
Fluorescent indicator. The non-fluorescent 6-CDCF DA passively diffuses across the cell membrane and is hydrolyzed to fluorescent CDCF by intracellular esterases. It has been shown to be highly sensitive, long-lasting, cell-permeant and fluorescein-based tracer for quantifying ROS generation. Substrate for MRP2 and MRP3 and therefore the function of the efflux transporters (hepatic exrection) and multi-drug resistance associated proteins MRP2 and MRP3 can be measured using 6-CDCF DA. The CDCFDA/CDCF technoique provides a fast screening tool for MRP2 substrates and/or inhibitors. Spectral data: λEx=502nm, λEm=530nm.
Synonyms | 6-CDCF DA, 6-Carboxy-DCF DA |
---|---|
Purity | ≥97% (NMR) |
Appearance | White to off-white powder |
CAS-Number | 144489-10-1 |
Molecular Formula | C25H14Cl2O9 |
Molecular Weight | 529.29 |
Identity | 1H-NMR |
Solvents | DMSO, chloroform, water (insoluble) |
Smiles | O=C(O1)C2=C(C=C(C(O)=O)C=C2)C31C4=C(C=C(OC(C)=O)C(Cl)=C4)OC5=CC(OC(C)=O)=C(Cl)C=C53 |
InChi Key | FCNONRRKVDBQRD-UHFFFAOYSA-N |
Shipping | AMBIENT |
Short Term Storage | +4°C |
Long Term Storage | -20°C |
Handling Advice | Protect from light and moisture. |
Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
Transportation | Not dangerous goods |
References | (1) J.P. Kehrer & T. Paraidathathu, Free Rad. Res. Commun. 16, 217 (1992), (2) M.J. Zamek-Gliszczynski, et al., J. Pharmacol. Exp. Ther. 304, 801 (2003), (3) S. Siissalo, et al., Eur. J. Pharm. Biopharm. 71, 332 (2009), (4) A.I. Pogue, et al., Int. J. Mol. Sci. 13, 9615 (2012), (5) J.A.Sinclair, et al., Toxicol. In Vitro 27, 451 (2013) |
InChi | InChI=1S/C25H14Cl2O9/c1-10(28)33-21-8-19-15(6-17(21)26)25(14-5-12(23(30)31)3-4-13(14)24(32)36-25)16-7-18(27)22(34-11(2)29)9-20(16)35-19/h3-9H,1-2H3,(H,30,31) |
Quantity | 100 mg, 250 mg, Bulk |