Specifications
- Purity
- ≥98% (HPLC)
- Appearance
- Liquid
- Identity
- 1H-NMR
- Concentration
- 1 mg/ml in water
Properties
- Solvents
- water
- Fluorescence
- λex 355 nm, λem 465 nm in TE buffer
Documentation
- Safety Data Sheet (SDS)
- CDX B0447 MSDS.pdf
Available from stock
Hoechst 33342 solution, 1 mg/ml in water
- SKU
- B0447
Categories: Fluorescent Detection, Fluorescent Detection
- Synonyms
- 2'-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bi-1H-benzimidazole trihydrochloride , BisBenzimide H 33342 , HOE 33342
- 23491-52-3
- CAS-Number
- C27H28N6O . 3HCl . xH2O
- Molecular Formula
- 561.93
- Molecular Weight
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
- Shipping
- AMBIENT
- Short Term Storage
- +4°C
- Long Term Storage
- +4°C
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at +4°C.
- Hazard statements
- H302-H315-H335-H341
- Precautionary statements
- P280-P301 + P312 + P330
- GHS Symbol
- GHS07+GHS08
- Signal word
- Warning
- Transportation
- Not dangerous goods
- Description
- The Hoechst stains are a family of fluorescent stains for labeling DNA in fluorescence microscopy. The blue fluorescent Hoechst dye is a cell permeable nucleic acid stain that has multiple applications, including sensitive detection of DNA in the presence of RNA in agarose gels, automated DNA determination, sensitive determination of cell number and chromosome sorting. Useful vital stain for the flow cytometric recognition of DNA damage and other viability measurements by monitoring the emission spectral shifts of the dyes. Because this fluorescent stain labels DNA, it can also be used to visualize nuclei and mitochondria. Hoechst 33342 is a cell-permeable, benzimidazole dye that stains DNA by binding to the minor groove of adenine and thymine-rich sequences. It emits blue fluorescence (excitation 350 nm/emission maximum 461 nm) when bound to double stranded DNA and is useful as a marker of nuclei for cell cycle studies and to distinguish nuclear morphology in apoptotic cells.
- Smiles
- CN(CC1)CCN1C2=CC=C(NC(C3=CC(NC(C4=CC=C(OCC)C=C4)=N5)=C5C=C3)=N6)C6=C2
- InChi Key
- PRDFBSVERLRRMY-UHFFFAOYSA-N
- References
- (1) D.J. Pierce et al.: Exp. Hematol. 35(9), 1437 (2007) , (2) M.E. Lalande et al.: Proc. Natl. Acad. Sci. USA 78, 363 (1981) , (3) M.J. Lydon et al.: J. Cell. Physiol. 102, 175 (1980)
- InChi
- InChI=1S/C27H28N6O/c1-3-34-21-8-4-18(5-9-21)26-28-22-10-6-19(16-24(22)30-26)27-29-23-11-7-20(17-25(23)31-27)33-14-12-32(2)13-15-33/h4-11,16-17H,3,12-15H2,1-2H3,(H,28,30)(H,29,31)
The Hoechst stains are a family of fluorescent stains for labeling DNA in fluorescence microscopy. The blue fluorescent Hoechst dye is a cell permeable nucleic acid stain that has multiple applications, including sensitive detection of DNA in the presence of RNA in agarose gels, automated DNA determination, sensitive determination of cell number and chromosome sorting. Useful vital stain for the flow cytometric recognition of DNA damage and other viability measurements by monitoring the emission spectral shifts of the dyes. Because this fluorescent stain labels DNA, it can also be used to visualize nuclei and mitochondria. Hoechst 33342 is a cell-permeable, benzimidazole dye that stains DNA by binding to the minor groove of adenine and thymine-rich sequences. It emits blue fluorescence (excitation 350 nm/emission maximum 461 nm) when bound to double stranded DNA and is useful as a marker of nuclei for cell cycle studies and to distinguish nuclear morphology in apoptotic cells.
Additional information
| Synonyms | 2'-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bi-1H-benzimidazole trihydrochloride, BisBenzimide H 33342, HOE 33342 |
|---|---|
| Purity | ≥98% (HPLC) |
| Appearance | Liquid |
| CAS-Number | 23491-52-3 |
| Molecular Formula | C27H28N6O . 3HCl . xH2O |
| Molecular Weight | 561.93 |
| Identity | 1H-NMR |
| Solvents | water |
| Fluorescence | λex 355 nm, λem 465 nm in TE buffer |
| Smiles | CN(CC1)CCN1C2=CC=C(NC(C3=CC(NC(C4=CC=C(OCC)C=C4)=N5)=C5C=C3)=N6)C6=C2 |
| InChi Key | PRDFBSVERLRRMY-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | +4°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at +4°C. |
| Hazard statements | H302-H315-H335-H341 |
| Precautionary statements | P280-P301 + P312 + P330 |
| GHS Symbol | GHS07+GHS08 |
| Signal word | Warning |
| Transportation | Not dangerous goods |
| References | (1) D.J. Pierce et al.: Exp. Hematol. 35(9), 1437 (2007), (2) M.E. Lalande et al.: Proc. Natl. Acad. Sci. USA 78, 363 (1981), (3) M.J. Lydon et al.: J. Cell. Physiol. 102, 175 (1980) |
| InChi | InChI=1S/C27H28N6O/c1-3-34-21-8-4-18(5-9-21)26-28-22-10-6-19(16-24(22)30-26)27-29-23-11-7-20(17-25(23)31-27)33-14-12-32(2)13-15-33/h4-11,16-17H,3,12-15H2,1-2H3,(H,28,30)(H,29,31) |
| Quantity | 5 ml, Bulk |
| cdx-Concentration | 1 mg/ml in water |
