Specifications
- Purity
- ≥90% (NMR)
- Appearance
- Red to Violet powder
- Identity
- 1H-NMR
Properties
- Solvents
- DMSO, DMF
Category: Fluorescent Detection
6-ROX N-succinimidyl ester (6-ROX-SE) is an amine-reactive long wavelength rhodamine dye. Derivatives made with the amine-reactive carboxy-X-rhodamine (ROX), succinimidyl ester are widely used for oligonucleotide labeling and automated DNA sequencing applications. Conjugates of this dye have longer-wavelength spectra than the spectra of Lissamine rhodamine B conjugates, but somewhat shorter-wavelength spectra than those of Texas Red® conjugates. 6-ROX-SE derivatized compounds such as proteins, nucleic acids and drugs may be analyzed by fluorescence resonance energy transfer (FRET) and fluorescence quenching applications. 6-ROX-SE is a labeling reagent for preparation of charge-modified dye-labeled ddNTPs to “direct-load” DNA sequencing. 6-ROX-SE is a single isomer of ROX and increasingly preferred for labeling peptides and nucleotides because it gives better resolution in HPLC purifications that are often required in bioconjugations. Variable ratios of the 5- and 6-isomers often cause complications in the interpretation of labeling results and assay performances. Spectral data: λex=578nm, λem=604nm, Extinction coefficient (cm-1M-1): 82000, Quantum yield: 0.94 (aqueous buffer pH 7.0).
Synonyms | 6-Carboxy-X-rhodamine succinimidyl ester, 6-ROX SE, 6-CXR SE |
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Purity | ≥90% (NMR) |
Appearance | Red to Violet powder |
CAS-Number | 216699-36-4 |
Molecular Formula | C37H33N3O7 |
Molecular Weight | 631.67 |
Identity | 1H-NMR |
Solvents | DMSO, DMF |
Smiles | [O-]C(=O)c1ccc(cc1-c2c3cc4CCCN5CCCc(c45)c3[o+]c6c7CCCN8CCCc(cc26)c78)C(=O)ON9C(=O)CCC9=O |
InChi Key | KWADUASJUMATIK-UHFFFAOYSA-N |
Shipping | AMBIENT |
Short Term Storage | +4°C |
Long Term Storage | -20°C |
Handling Advice | Protect from light and moisture. |
Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
Hazard statements | H302,H315,H319,H335 |
Precautionary statements | P280,P305+P351+P338 |
GHS Symbol | GHS07 |
Signal word | Warning |
Transportation | Not dangerous goods |
References | (1) L.G. Lee, et al., Nucl. Acids Res. 20, 2471 (1992), (2) J. Ju, et al., PNAS 92, 4347 (1995), (3) S.-C. Hung, et al., Anal. Biochem. 255, 32 (1998), (4) Y. Yoshikawa, et al., Anal. Biochem. 256, 82 (1998), (5) P.J. Finn, et al., Nucl. Acids Res. 30, 2877 (2002), (6) T.S. Seo, et al., PNAS 102, 5926 (2005) |
InChi | InChI=1S/C37H33N3O7/c41-29-11-12-30(42)40(29)47-37(45)22-9-10-23(36(43)44)26(19-22)31-27-17-20-5-1-13-38-15-3-7-24(32(20)38)34(27)46-35-25-8-4-16-39-14-2-6-21(33(25)39)18-28(31)35/h9-10,17-19H,1-8,11-16H2 |
Quantity | 5 mg, Bulk |
cdx-Emission max. | 600±7 nm |