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7-Chlorobenzofurazan-4-sulfonic acidammonium salt

SKU: A0052

Category: Fluorescent Detection

Synonyms: SBF-Cl, SBF-Chloride, 4-Chloro-7-sulfobenzofurazan ammonium salt, 4-Chloro-7-sulfobenz-2-oxa-1,3-diazole ammonium salt, 7-Chloro-4-benzofurazansulfonic acid ammonium salt; 81377-14-2
CAS-Number: C₆H₃ClN₂O₄S · NH₃
Molecular Formula: 251.65
Molecular Weight

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥97% (NMR)
Appearance: Off-white to brown powder
Identity: 1H-NMR

Properties

Solvents: Soluble in water (50 mg/ml).
Melting Point: >300 °C (lit.) decomp. without melting
Fluorescence: λex 360 nm; λem 410 nm (pH 8.5)λex 383 nm; λem 528 nm (Reaction product)

Shipping: AMBIENT
Short Term Storage: +4°C
Long Term Storage: -20°C
Handling Advice: Protect from light and moisture.
Use / Stability: Stable for at least 2 years after receipt when stored at -20°C.

Hazard statements: H315 - H319 - H335
Precautionary statements: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
GHS Symbol: GHS07
Signal word: Warning
Transportation: Not dangerous goods

Description: Ammonium 4-chloro-7-sulfobenzofurazan (SBF-Cl) is a water-soluble fluorogenic reagent which is highly specific for thiol groups. SBF-Cl is a very good substrate for the investigation of glutathione S-transferases (GST). Used as a hematology and histology stain and in diagnostic assay manufacturing. Spectral data: ?ex 360 nm, ?em 410 nm (pH 8.5), ?ex 383 nm, ?em 528 nm (Reaction product).
Smiles: ClC1=CC=C(S(=O)([O-])=O)C2=NON=C21.[NH4+]
InChi Key: QFHXPBLOMHGYOC-UHFFFAOYSA-N
References: (1) J.L. Andrews, et al., Arch. Biochem. Biophys. 214, 386 (1982) , (2) R.M. Bolton, et al., Anal. Biochem. 216, 418 (1994) , (3) X.P. Chen, et al., J. Chromatogr. B: Biom. Sci. Appl. 709, 19 (1998)

Ammonium 4-chloro-7-sulfobenzofurazan (SBF-Cl) is a water-soluble fluorogenic reagent which is highly specific for thiol groups. SBF-Cl is a very good substrate for the investigation of glutathione S-transferases (GST). Used as a hematology and histology stain and in diagnostic assay manufacturing. Spectral data: ?ex 360 nm, ?em 410 nm (pH 8.5), ?ex 383 nm, ?em 528 nm (Reaction product).

SKU: A0052 Category: Fluorescent Detection

Additional information

Additional information

Synonyms	SBF-Cl, SBF-Chloride, 4-Chloro-7-sulfobenzofurazan ammonium salt, 4-Chloro-7-sulfobenz-2-oxa-1,3-diazole ammonium salt, 7-Chloro-4-benzofurazansulfonic acid ammonium salt
Purity	≥97% (NMR)
Appearance	Off-white to brown powder
CAS-Number	81377-14-2
Molecular Formula	C6H3ClN2O4S · NH3
Molecular Weight	251.65
Identity	1H-NMR
Solvents	Soluble in water (50 mg/ml).
Melting Point	>300 °C (lit.) decomp. without melting
Fluorescence	λex 360 nm; λem 410 nm (pH 8.5)λex 383 nm; λem 528 nm (Reaction product)
Smiles	ClC1=CC=C(S(=O)([O-])=O)C2=NON=C21.[NH4+]
InChi Key	QFHXPBLOMHGYOC-UHFFFAOYSA-N
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at -20°C.
Hazard statements	H315 – H319 – H335
Precautionary statements	P261 – P264 – P271 – P280 – P302 + P352 – P305 + P351 + P338
GHS Symbol	GHS07
Signal word	Warning
Transportation	Not dangerous goods
References	(1) J.L. Andrews, et al., Arch. Biochem. Biophys. 214, 386 (1982), (2) R.M. Bolton, et al., Anal. Biochem. 216, 418 (1994), (3) X.P. Chen, et al., J. Chromatogr. B: Biom. Sci. Appl. 709, 19 (1998)
Quantity	100 mg, Bulk