Specifications
- Purity
- ≥98%
- Appearance
- White to off-white powder
- Identity
- 1H-NMR
Properties
- Solvents
- DMSO (20 mg/ml), DMF (20 mg/ml)
Documentation
- Safety Data Sheet (SDS)
- CDX B0217 MSDS.pdf
Available from stock
BAPTA
- SKU
- B0217
Categories: Fluorescent Detection, Fluorescent Detection
- Synonyms
- 1,2-Bis(2-aminophenoxy)ethane-N,N,N’,N’-tetraacetic acid
- 85233-19-8
- CAS-Number
- C22H24N2O10
- Molecular Formula
- 476.4
- Molecular Weight
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
- Shipping
- AMBIENT
- Short Term Storage
- +4°C
- Long Term Storage
- +4°C
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at +4°C.
- Transportation
- Not dangerous goods
- Description
- BAPTA is a membrane-impermeable highly selective calcium chelator and is useful for manipulating extracellular Ca2+ levels, with 105-fold greater affinity for Ca2+ than for Mg2+. The presence of four carboxylic acid functional groups allows for the binding of two calcium ions. BAPTA and its derivatives can be used as calcium indicators, since the absorption maximum for BAPTA changes when it is complexed with calcium (absorption maxima free/complexed = 254/274 nm, emission maxima free/complexed = 363/363 nm). Key advantages of BAPTA include relative insensitivity toward intracellular pH change and fast release of calcium. This product is a high quality and sensitive compound for calcium signaling studies, investigation of signal transduction, apoptotic cascades and neuroscience research.
- Smiles
- O=C(O)CN(CC(O)=O)C1=C(OCCOC2=C(N(CC(O)=O)CC(O)=O)C=CC=C2)C=CC=C1
- InChi Key
- FTEDXVNDVHYDQW-UHFFFAOYSA-N
- References
- (1) R.Y. Tsien, et al., Biochemistry. 19, 2396 (1980) , (2) R.Y. Tsien, et al., Annu. Rev. Biophys. Bioeng. 12, 91 (1983) , (3) G. Grynkiewicz, et al., J. Biol. Chem. 260, 3440 (1985) , (4) H. Kijima, et al., J. Physiol. 403, 135 (1988) , (5) N. Tanabe, et al., Neurosci. Lett. 92, 52 (1988) , (6) R.C. Hardie, Cell Calcium. 38, 547 (2005) , (7) R.W. Sabnis, Handbook of biological dyes and stains (2010)
- InChi
- InChI=1S/C22H24N2O10/c25-19(26)11-23(12-20(27)28)15-5-1-3-7-17(15)33-9-10-34-18-8-4-2-6-16(18)24(13-21(29)30)14-22(31)32/h1-8H,9-14H2,(H,25,26)(H,27,28)(H,29,30)(H,31,32)
BAPTA is a membrane-impermeable highly selective calcium chelator and is useful for manipulating extracellular Ca2+ levels, with 105-fold greater affinity for Ca2+ than for Mg2+. The presence of four carboxylic acid functional groups allows for the binding of two calcium ions. BAPTA and its derivatives can be used as calcium indicators, since the absorption maximum for BAPTA changes when it is complexed with calcium (absorption maxima free/complexed = 254/274 nm, emission maxima free/complexed = 363/363 nm). Key advantages of BAPTA include relative insensitivity toward intracellular pH change and fast release of calcium. This product is a high quality and sensitive compound for calcium signaling studies, investigation of signal transduction, apoptotic cascades and neuroscience research.
Additional information
| Synonyms | 1,2-Bis(2-aminophenoxy)ethane-N,N,N’,N’-tetraacetic acid |
|---|---|
| Purity | ≥98% |
| Appearance | White to off-white powder |
| CAS-Number | 85233-19-8 |
| Molecular Formula | C22H24N2O10 |
| Molecular Weight | 476.4 |
| Identity | 1H-NMR |
| Solvents | DMSO (20 mg/ml), DMF (20 mg/ml) |
| Smiles | O=C(O)CN(CC(O)=O)C1=C(OCCOC2=C(N(CC(O)=O)CC(O)=O)C=CC=C2)C=CC=C1 |
| InChi Key | FTEDXVNDVHYDQW-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | +4°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at +4°C. |
| Transportation | Not dangerous goods |
| References | (1) R.Y. Tsien, et al., Biochemistry. 19, 2396 (1980), (2) R.Y. Tsien, et al., Annu. Rev. Biophys. Bioeng. 12, 91 (1983), (3) G. Grynkiewicz, et al., J. Biol. Chem. 260, 3440 (1985), (4) H. Kijima, et al., J. Physiol. 403, 135 (1988), (5) N. Tanabe, et al., Neurosci. Lett. 92, 52 (1988), (6) R.C. Hardie, Cell Calcium. 38, 547 (2005), (7) R.W. Sabnis, Handbook of biological dyes and stains (2010) |
| InChi | InChI=1S/C22H24N2O10/c25-19(26)11-23(12-20(27)28)15-5-1-3-7-17(15)33-9-10-34-18-8-4-2-6-16(18)24(13-21(29)30)14-22(31)32/h1-8H,9-14H2,(H,25,26)(H,27,28)(H,29,30)(H,31,32) |
| Quantity | 250 mg, 1 g, 5 g, Bulk |
