Available from stock

Biotin-(5-fluorescein)-conjugate

SKU: B0078

Category: Fluorescent Detection

Synonyms: Biotin-4-Fluorescein , B4F , N-(Biotinylamidoethyl)-fluorescein-5-carboxamide , N-(Fluorescein-5-carbamidoethyl)biotinamide , 225241-94-1, 1260072-13-6, 1032732-74-3; 957494-27-8
CAS-Number: C₃₃H₃₂N₄O₈S
Molecular Formula: 644.70
Molecular Weight

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥90% (HPLC)
Appearance: Yellow solid
Purity 2: ≥90% (TLC)

Properties

Solvents: DMSO
UV / Vis: λmax 494 ± 3 nm (buffer pH10)
Fluorescence: λmax 524 ± 4 nm (buffer pH10)

Documentation

Safety Data Sheet (SDS): CDX B0078 MSDS.pdf

Shipping: AMBIENT
Short Term Storage: +4°C
Long Term Storage: -20°C
Handling Advice: Protect from light and moisture.
Use / Stability: Stable for at least 2 years after receipt when stored at -20°C.

Transportation: Not dangerous goods

Description: Biotin-fluorescein conjugate for the fluorometric accurate measurement and quantification of avidin and streptavidin. Shows exceptionally fast and tight binding to avidin and streptavidin accompanied by strong quenching. Used in spectrophotometric streptavidin-biotin binding assays. May be used in situations similar to fluorescein isothiocyanate staining to label avidin/streptavidin conjugated molecules such as antibodies with the fluorophore 5-fluorescein. Spectral Data: λex 490nm, λem 524nm in 0.1M Tris pH8.0.
Smiles: O=C1C=C2OC3=CC(O)=CC=C3C(C4=C(C(O)=O)C=C(C(NCCNC(CCCC[C@H]5[C@](NC(N6)=O)([H])[C@]6([H])CS5)=O)=O)C=C4)=C2C=C1
InChi Key: DFUFXKZUEOKPSD-LFERIPGTSA-N
References: (1) G. Kada et al. Biochim. Biophys. Acta 1427, 33 (1999) , (2) G. Kada et al. Biochim. Biophys. Acta 1427, 44 (1999) , (3) N. Humbert, et al., Electrophoresis 26, 47 (2005) , (4) F.M. Aslan, et al., PNAS 102, 8507 (2005) , (5) M.J. Waner & D.P. Mascotti, J. Biochem. Biophys. Methods 70, 873 (2008) , (6) A. Ebner, et al., Methods Mol. Biol. 418, 73 (2008) , (7) R. Mittal & M.P. Bruchez, Bioconjug. Chem. 22, 362 (2011)
InChi: InChI=1S/C33H32N4O8S/c38-18-6-9-21-25(14-18)45-26-15-19(39)7-10-22(26)29(21)20-8-5-17(13-23(20)32(42)43)31(41)35-12-11-34-28(40)4-2-1-3-27-30-24(16-46-27)36-33(44)37-30/h5-10,13-15,24,27,30,38H,1-4,11-12,16H2,(H,34,40)(H,35,41)(H,42,43)(H2,36,37,44)/t24-,27-,30-/m0/s1

Biotin-fluorescein conjugate for the fluorometric accurate measurement and quantification of avidin and streptavidin. Shows exceptionally fast and tight binding to avidin and streptavidin accompanied by strong quenching. Used in spectrophotometric streptavidin-biotin binding assays. May be used in situations similar to fluorescein isothiocyanate staining to label avidin/streptavidin conjugated molecules such as antibodies with the fluorophore 5-fluorescein. Spectral Data: λex 490nm, λem 524nm in 0.1M Tris pH8.0.

SKU: B0078 Category: Fluorescent Detection

Additional information

Additional information

Synonyms	Biotin-4-Fluorescein, B4F, N-(Biotinylamidoethyl)-fluorescein-5-carboxamide, N-(Fluorescein-5-carbamidoethyl)biotinamide, 225241-94-1, 1260072-13-6, 1032732-74-3
Purity	≥90% (HPLC)
Appearance	Yellow solid
CAS-Number	957494-27-8
Molecular Formula	C33H32N4O8S
Molecular Weight	644.70
Solvents	DMSO
UV / Vis	λmax 494 ± 3 nm (buffer pH10)
Fluorescence	λmax 524 ± 4 nm (buffer pH10)
Smiles	O=C1C=C2OC3=CC(O)=CC=C3C(C4=C(C(O)=O)C=C(C(NCCNC(CCCC[C@H]5[C@](NC(N6)=O)([H])[C@]6([H])CS5)=O)=O)C=C4)=C2C=C1
InChi Key	DFUFXKZUEOKPSD-LFERIPGTSA-N
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at -20°C.
Transportation	Not dangerous goods
References	(1) G. Kada et al. Biochim. Biophys. Acta 1427, 33 (1999), (2) G. Kada et al. Biochim. Biophys. Acta 1427, 44 (1999), (3) N. Humbert, et al., Electrophoresis 26, 47 (2005), (4) F.M. Aslan, et al., PNAS 102, 8507 (2005), (5) M.J. Waner & D.P. Mascotti, J. Biochem. Biophys. Methods 70, 873 (2008), (6) A. Ebner, et al., Methods Mol. Biol. 418, 73 (2008), (7) R. Mittal & M.P. Bruchez, Bioconjug. Chem. 22, 362 (2011)
InChi	InChI=1S/C33H32N4O8S/c38-18-6-9-21-25(14-18)45-26-15-19(39)7-10-22(26)29(21)20-8-5-17(13-23(20)32(42)43)31(41)35-12-11-34-28(40)4-2-1-3-27-30-24(16-46-27)36-33(44)37-30/h5-10,13-15,24,27,30,38H,1-4,11-12,16H2,(H,34,40)(H,35,41)(H,42,43)(H2,36,37,44)/t24-,27-,30-/m0/s1
Quantity	1 mg, 5 mg, Bulk
cdx-Purity 2	≥90% (TLC)