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      Biotin-(5-fluorescein)-conjugate

      SKU
      B0078

      Category: Fluorescent Detection

      Synonyms
      Biotin-4-Fluorescein , B4F , N-(Biotinylamidoethyl)-fluorescein-5-carboxamide , N-(Fluorescein-5-carbamidoethyl)biotinamide
      957494-27-8
      CAS-Number
      C33H32N4O8S
      Molecular Formula
      644.70
      Molecular Weight

      • Product Data
      • Handling
      • Safety Information
      • Details

      Specifications

      Purity
      ≥90% (HPLC)
      Appearance
      Yellow solid
      Purity 2
      ≥90% (TLC)

      Properties

      Solvents
      DMSO

      Downloads

      Safety Data Sheet
      CDX B0078 MSDS.pdf
      Long Term Storage
      -20°C
      Use / Stability
      Stable for at least 2 years after receipt when stored at -20°C.
      Handling Advice
      Protect from light and moisture.
      Short Term Storage
      +4°C
      Shipping
      AMBIENT
      Transportation
      Not dangerous goods
      Description
      Biotin-fluorescein conjugate for the fluorometric accurate measurement and quantification of avidin and streptavidin. Shows exceptionally fast and tight binding to avidin and streptavidin accompanied by strong quenching. Used in spectrophotometric streptavidin-biotin binding assays. May be used in situations similar to fluorescein isothiocyanate staining to label avidin/streptavidin conjugated molecules such as antibodies with the fluorophore 5-fluorescein. Spectral Data: λex 490nm, λem 524nm in 0.1M Tris pH8.0.
      InChi
      InChI=1S/C33H32N4O8S/c38-18-6-9-21-25(14-18)45-26-15-19(39)7-10-22(26)29(21)20-8-5-17(13-23(20)32(42)43)31(41)35-12-11-34-28(40)4-2-1-3-27-30-24(16-46-27)36-33(44)37-30/h5-10,13-15,24,27,30,38H,1-4,11-12,16H2,(H,34,40)(H,35,41)(H,42,43)(H2,36,37,44)/t24-,27-,30-/m0/s1
      References
      (1) G. Kada et al. Biochim. Biophys. Acta 1427, 33 (1999) , (2) G. Kada et al. Biochim. Biophys. Acta 1427, 44 (1999) , (3) N. Humbert, et al., Electrophoresis 26, 47 (2005) , (4) F.M. Aslan, et al., PNAS 102, 8507 (2005) , (5) M.J. Waner & D.P. Mascotti, J. Biochem. Biophys. Methods 70, 873 (2008) , (6) A. Ebner, et al., Methods Mol. Biol. 418, 73 (2008) , (7) R. Mittal & M.P. Bruchez, Bioconjug. Chem. 22, 362 (2011)
      InChi Key
      DFUFXKZUEOKPSD-LFERIPGTSA-N
      Smiles
      O=C1C=C2OC3=CC(O)=CC=C3C(C4=C(C(O)=O)C=C(C(NCCNC(CCCC[C@H]5[C@](NC(N6)=O)([H])[C@]6([H])CS5)=O)=O)C=C4)=C2C=C1

      Biotin-fluorescein conjugate for the fluorometric accurate measurement and quantification of avidin and streptavidin. Shows exceptionally fast and tight binding to avidin and streptavidin accompanied by strong quenching. Used in spectrophotometric streptavidin-biotin binding assays. May be used in situations similar to fluorescein isothiocyanate staining to label avidin/streptavidin conjugated molecules such as antibodies with the fluorophore 5-fluorescein. Spectral Data: λex 490nm, λem 524nm in 0.1M Tris pH8.0.

      SKU: B0078 Category: Fluorescent Detection
      • Additional information

      Additional information

      Synonyms

      Biotin-4-Fluorescein, B4F, N-(Biotinylamidoethyl)-fluorescein-5-carboxamide, N-(Fluorescein-5-carbamidoethyl)biotinamide

      Purity

      ≥90% (HPLC)

      Appearance

      Yellow solid

      CAS-Number

      957494-27-8

      Molecular Formula

      C33H32N4O8S

      Molecular Weight

      644.70

      Solvents

      DMSO

      Smiles

      O=C1C=C2OC3=CC(O)=CC=C3C(C4=C(C(O)=O)C=C(C(NCCNC(CCCC[C@H]5[C@](NC(N6)=O)([H])[C@]6([H])CS5)=O)=O)C=C4)=C2C=C1

      InChi Key

      DFUFXKZUEOKPSD-LFERIPGTSA-N

      Shipping

      AMBIENT

      Short Term Storage

      +4°C

      Long Term Storage

      -20°C

      Handling Advice

      Protect from light and moisture.

      Use / Stability

      Stable for at least 2 years after receipt when stored at -20°C.

      Transportation

      Not dangerous goods

      References

      (1) G. Kada et al. Biochim. Biophys. Acta 1427, 33 (1999), (2) G. Kada et al. Biochim. Biophys. Acta 1427, 44 (1999), (3) N. Humbert, et al., Electrophoresis 26, 47 (2005), (4) F.M. Aslan, et al., PNAS 102, 8507 (2005), (5) M.J. Waner & D.P. Mascotti, J. Biochem. Biophys. Methods 70, 873 (2008), (6) A. Ebner, et al., Methods Mol. Biol. 418, 73 (2008), (7) R. Mittal & M.P. Bruchez, Bioconjug. Chem. 22, 362 (2011)

      InChi

      InChI=1S/C33H32N4O8S/c38-18-6-9-21-25(14-18)45-26-15-19(39)7-10-22(26)29(21)20-8-5-17(13-23(20)32(42)43)31(41)35-12-11-34-28(40)4-2-1-3-27-30-24(16-46-27)36-33(44)37-30/h5-10,13-15,24,27,30,38H,1-4,11-12,16H2,(H,34,40)(H,35,41)(H,42,43)(H2,36,37,44)/t24-,27-,30-/m0/s1

      Quantity

      1 mg, 5 mg, Bulk

      cdx-Purity 2

      ≥90% (TLC)

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