Specifications
- Purity
- ≥90% (HPLC)
- Appearance
- Liquid
- Identity
- 1H-NMR
Properties
- Solvents
- chloroform, DMSO
- Fluorescence
- λex 495 nm, λem 515 nm
Available from stock
DAF-4 DA solution (5 mM in DMSO), 1 mg in 0.39 ml DMSO
- SKU
- D0516
Categories: Fluorescent Detection, Fluorescent Detection
- Synonyms
- DAF-4 diacetate solution , 3,4-Diamino-2',7'-dichlorofluorescein diacetate solution
- 208850-34-4
- CAS-Number
- C24H16Cl2N2O7
- Molecular Formula
- 515.30
- Molecular Weight
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
- Shipping
- AMBIENT
- Short Term Storage
- +4°C
- Long Term Storage
- -20°C
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at -20°C.
- Transportation
- Not dangerous goods
- Description
- DAF-4 is a indicator for nitric oxide. As with other fluorescein diacetates (DAFs), the non-fluorescent DAF-4 diacetate (DAF-4 DA) is membrane permeant and is deacetylated by intracellular esterases to form DAF-4, which than can ract with NO to form the fluorescent DAF-4T. Can be used for the fluorimetric detection of nitric oxide and in fluorescence microscopy to measure real-time changes in nitric oxide levels in vivo. Spectral Data: λex=505nm, λem=530nm.
- Smiles
- ClC1=C(OC(C)=O)C=C(OC(C=C(OC(C)=O)C(Cl)=C2)=C2C34OC(C5=C4C=C(N)C(N)=C5)=O)C3=C1
- InChi Key
- OOSGYDWTQGDKAY-UHFFFAOYSA-N
- References
- (1) H. Kojima, et al., Anal. Chem. 70, 2446 (1998) , (2) N. Nakatsubo, et al., FEBS Lett. 427, 263 (1998) , (3) T. Nagano, Luminescence 14, 283 (1999) (Review) , (4) T. Nagano, Proc. Jpn. Acad. Ser. B 86, 837 (2010) (Review)
- InChi
- InChI=1S/C24H16Cl2N2O7/c1-9(29)32-21-7-19-13(4-15(21)25)24(12-6-18(28)17(27)3-11(12)23(31)35-24)14-5-16(26)22(33-10(2)30)8-20(14)34-19/h3-8H,27-28H2,1-2H3
DAF-4 is a indicator for nitric oxide. As with other fluorescein diacetates (DAFs), the non-fluorescent DAF-4 diacetate (DAF-4 DA) is membrane permeant and is deacetylated by intracellular esterases to form DAF-4, which than can ract with NO to form the fluorescent DAF-4T. Can be used for the fluorimetric detection of nitric oxide and in fluorescence microscopy to measure real-time changes in nitric oxide levels in vivo. Spectral Data: λex=505nm, λem=530nm.
Additional information
| Synonyms | DAF-4 diacetate solution, 3,4-Diamino-2',7'-dichlorofluorescein diacetate solution |
|---|---|
| Purity | ≥90% (HPLC) |
| Appearance | Liquid |
| CAS-Number | 208850-34-4 |
| Molecular Formula | C24H16Cl2N2O7 |
| Molecular Weight | 515.30 |
| Identity | 1H-NMR |
| Solvents | chloroform, DMSO |
| Fluorescence | λex 495 nm, λem 515 nm |
| Smiles | ClC1=C(OC(C)=O)C=C(OC(C=C(OC(C)=O)C(Cl)=C2)=C2C34OC(C5=C4C=C(N)C(N)=C5)=O)C3=C1 |
| InChi Key | OOSGYDWTQGDKAY-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Transportation | Not dangerous goods |
| References | (1) H. Kojima, et al., Anal. Chem. 70, 2446 (1998), (2) N. Nakatsubo, et al., FEBS Lett. 427, 263 (1998), (3) T. Nagano, Luminescence 14, 283 (1999) (Review), (4) T. Nagano, Proc. Jpn. Acad. Ser. B 86, 837 (2010) (Review) |
| InChi | InChI=1S/C24H16Cl2N2O7/c1-9(29)32-21-7-19-13(4-15(21)25)24(12-6-18(28)17(27)3-11(12)23(31)35-24)14-5-16(26)22(33-10(2)30)8-20(14)34-19/h3-8H,27-28H2,1-2H3 |
| Quantity | 1 mg, Bulk |
