Specifications
- Purity
- ≥98% (HPLC)
- Appearance
- Clear, colorless liquid
- Identity
- 1H-NMR
Properties
- Solvents
- DMSO
Categories: Fluorescent Detection, Fluorescent Detection
DAF-FM DA is a cell-permeable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM and DAF-FM DA are more sensitive to nitric oxide (NO) compared with DAF-2 and more photo-stable and less pH sensitive. DAF-FM DA permeates well into living cells and is rapidly transformed into water-soluble DAF-FM by cytosolic esterases.
Synonyms | 4-Amino-5-methylamino- 2',7'-difluorofluorescein diacetate, Diaminofluorescein-FM diacetate |
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Purity | ≥98% (HPLC) |
Appearance | Clear, colorless liquid |
CAS-Number | 254109-22-3 |
Molecular Formula | C25H18F2N2O7 |
Molecular Weight | 496.42 |
Identity | 1H-NMR |
Solvents | DMSO |
Smiles | FC1=CC2=C(C=C1OC(C)=O)OC3=C(C2(OC4=O)C5=C4C(N)=C(NC)C=C5)C=C(F)C(OC(C)=O)=C3 |
InChi Key | BEVHTVRRVVEMEF-UHFFFAOYSA-N |
Shipping | AMBIENT |
Short Term Storage | +4°C |
Long Term Storage | -20°C |
Handling Advice | Protect from light and moisture. |
Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
Hazard statements | H227 |
Transportation | Not dangerous goods |
References | (1) J. S. Carroll et al., Anal. Chem. 79(14), 5133 (2007), (2) Y. Itoh et al., Anal. Biochem. 287(2), 203 (2000), (3) H. Kojima et al., Angew. Chem. 111(219), 3419 (1999) |
InChi | InChI=1S/C25H18F2N2O7/c1-10(30)33-20-8-18-13(6-15(20)26)25(12-4-5-17(29-3)23(28)22(12)24(32)36-25)14-7-16(27)21(34-11(2)31)9-19(14)35-18/h4-9,29H,28H2,1-3H3 |
Quantity | 1 mg, Bulk |