Specifications
- Purity
- ≥98% (HPLC)
- Appearance
- Clear, colorless liquid
- Identity
- 1H-NMR
Properties
- Solvents
- DMSO
Available from stock
DAF-FM DA solution (5 mM in DMSO), 1 mg in 0.40 ml DMSO
- SKU
- A0524
Categories: Fluorescent Detection, Fluorescent Detection
- Synonyms
- 4-Amino-5-methylamino- 2',7'-difluorofluorescein diacetate, Diaminofluorescein-FM diacetate
- 254109-22-3
- CAS-Number
- C25H18F2N2O7
- Molecular Formula
- 496.42
- Molecular Weight
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
- Shipping
- AMBIENT
- Short Term Storage
- +4°C
- Long Term Storage
- -20°C
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at -20°C.
- Hazard statements
- H227
- Transportation
- Not dangerous goods
- Description
- DAF-FM DA is a cell-permeable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM and DAF-FM DA are more sensitive to nitric oxide (NO) compared with DAF-2 and more photo-stable and less pH sensitive. DAF-FM DA permeates well into living cells and is rapidly transformed into water-soluble DAF-FM by cytosolic esterases.
- Smiles
- FC1=CC2=C(C=C1OC(C)=O)OC3=C(C2(OC4=O)C5=C4C(N)=C(NC)C=C5)C=C(F)C(OC(C)=O)=C3
- InChi Key
- BEVHTVRRVVEMEF-UHFFFAOYSA-N
- References
- (1) J. S. Carroll et al., Anal. Chem. 79(14), 5133 (2007) , (2) Y. Itoh et al., Anal. Biochem. 287(2), 203 (2000) , (3) H. Kojima et al., Angew. Chem. 111(219), 3419 (1999)
- InChi
- InChI=1S/C25H18F2N2O7/c1-10(30)33-20-8-18-13(6-15(20)26)25(12-4-5-17(29-3)23(28)22(12)24(32)36-25)14-7-16(27)21(34-11(2)31)9-19(14)35-18/h4-9,29H,28H2,1-3H3
DAF-FM DA is a cell-permeable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM and DAF-FM DA are more sensitive to nitric oxide (NO) compared with DAF-2 and more photo-stable and less pH sensitive. DAF-FM DA permeates well into living cells and is rapidly transformed into water-soluble DAF-FM by cytosolic esterases.
Additional information
| Synonyms | 4-Amino-5-methylamino- 2',7'-difluorofluorescein diacetate, Diaminofluorescein-FM diacetate |
|---|---|
| Purity | ≥98% (HPLC) |
| Appearance | Clear, colorless liquid |
| CAS-Number | 254109-22-3 |
| Molecular Formula | C25H18F2N2O7 |
| Molecular Weight | 496.42 |
| Identity | 1H-NMR |
| Solvents | DMSO |
| Smiles | FC1=CC2=C(C=C1OC(C)=O)OC3=C(C2(OC4=O)C5=C4C(N)=C(NC)C=C5)C=C(F)C(OC(C)=O)=C3 |
| InChi Key | BEVHTVRRVVEMEF-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Hazard statements | H227 |
| Transportation | Not dangerous goods |
| References | (1) J. S. Carroll et al., Anal. Chem. 79(14), 5133 (2007), (2) Y. Itoh et al., Anal. Biochem. 287(2), 203 (2000), (3) H. Kojima et al., Angew. Chem. 111(219), 3419 (1999) |
| InChi | InChI=1S/C25H18F2N2O7/c1-10(30)33-20-8-18-13(6-15(20)26)25(12-4-5-17(29-3)23(28)22(12)24(32)36-25)14-7-16(27)21(34-11(2)31)9-19(14)35-18/h4-9,29H,28H2,1-3H3 |
| Quantity | 1 mg, Bulk |
