Available from stock

Hoechst 33258

SKU: B0029

Categories: Fluorescent Detection, Fluorescent Detection

Synonyms: 4-[5-(4-Methyl-1-piperazinyl)-1H,1'H-2,5'-bibenzimidazol-2'-yl]phenol trihydrochloride; 23491-45-4
CAS-Number: C₂₅H₂₄N₆O . ₃HCl
Molecular Formula: 533.88
Molecular Weight

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥98% (HPLC, TLC)
Appearance: Yellow solid
Identity: 1H-NMR

Properties

Solvents: water, ethanol, DMSO
Melting Point: >300 °C (lit.)
Fluorescence: λex 355 nm, λem 465 nm in TE buffer

Documentation

Safety Data Sheet (SDS): CDX B0029 MSDS_V1.2.pdf

Shipping: AMBIENT
Short Term Storage: +4°C
Long Term Storage: +4°C
Handling Advice: Protect from light and moisture.
Use / Stability: Stable for at least 2 years after receipt when stored at +4°C.

Hazard statements: H302, H315, H319
Precautionary statements: P305 + P351 + P338
GHS Symbol: GHS07
Signal word: Warning
Transportation: Not dangerous goods

Description

Useful for staining DNA, chromosomes and nuclei. Binds to the minor groove of DNA at AT-rich sequences. This dye is commonly used for determining the DNA content of viable cells without detergent treatment or fixation and for fluorescence microscopy or flow cytometry.

Other available Hoechst dyes:

Name	Hoechst 33342	Hoechst 33258	Hoechst 34580	Hoechst S769121 (Nuclear Yellow)
SKU	B0030	B0029	B0094	H0328
CAS	875756-97-1	23491-45-4	911004-45-0	74681-68-8
Ex/Em (nm)	352/454	352/454	371/438	372/504
Molecular Weight	561.93	533.88	560.95	596.96

Smiles

CN1CCN(CC1)C1=CC=C2NC(=NC2=C1)C1=CC=C2NC(=NC2=C1)C1=CC=C(O)C=C1

InChi Key

INAAIJLSXJJHOZ-UHFFFAOYSA-N

References

(1) A. Kumar et al.: In Vitro Cell. & Developm. Biol. 44(7), 189 (2008) , (2) D. Weidmann et al.: Meth. Enzym. 446, 277 (2008) , (3) D. Plesca et al.: Meth. Enzym. 446, 107 (2008) , (4) K.H. Elstein et al.: Exp. Cell. Res. 211, 322 (1994) , (5) Y.-J. Kim et al.: Anal. Biochem. 174, 168 (1988) , (6) C. Labarca et al.: Anal. Biochem. 102, 344 (1980) , (7) S.A. Latt et al.: J. Histochem. Cytochem. 24, 24 (1976)

InChi

InChI=1S/C25H24N6O/c1-30-10-12-31(13-11-30)18-5-9-21-23(15-18)29-25(27-21)17-4-8-20-22(14-17)28-24(26-20)16-2-6-19(32)7-3-16/h2-9,14-15,32H,10-13H2,1H3,(H,26,28)(H,27,29)

Name	Hoechst 33342	Hoechst 33258	Hoechst 34580	Hoechst S769121 (Nuclear Yellow)
SKU	B0030	B0029	B0094	H0328
CAS	875756-97-1	23491-45-4	911004-45-0	74681-68-8
Ex/Em (nm)	352/454	352/454	371/438	372/504
Molecular Weight	561.93	533.88	560.95	596.96

SKU: B0029 Categories: Fluorescent Detection, Fluorescent Detection

Description
Additional information

Description

Additional information

Synonyms	4-[5-(4-Methyl-1-piperazinyl)-1H,1'H-2,5'-bibenzimidazol-2'-yl]phenol trihydrochloride
Purity	≥98% (HPLC, TLC)
Appearance	Yellow solid
CAS-Number	23491-45-4
Molecular Formula	C25H24N6O . 3HCl
Molecular Weight	533.88
Identity	1H-NMR
Solvents	water, ethanol, DMSO
Melting Point	>300 °C (lit.)
Fluorescence	λex 355 nm, λem 465 nm in TE buffer
Smiles	CN1CCN(CC1)C1=CC=C2NC(=NC2=C1)C1=CC=C2NC(=NC2=C1)C1=CC=C(O)C=C1
InChi Key	INAAIJLSXJJHOZ-UHFFFAOYSA-N
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	+4°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at +4°C.
Hazard statements	H302, H315, H319
Precautionary statements	P305 + P351 + P338
GHS Symbol	GHS07
Signal word	Warning
Transportation	Not dangerous goods
References	(1) A. Kumar et al.: In Vitro Cell. & Developm. Biol. 44(7), 189 (2008), (2) D. Weidmann et al.: Meth. Enzym. 446, 277 (2008), (3) D. Plesca et al.: Meth. Enzym. 446, 107 (2008), (4) K.H. Elstein et al.: Exp. Cell. Res. 211, 322 (1994), (5) Y.-J. Kim et al.: Anal. Biochem. 174, 168 (1988), (6) C. Labarca et al.: Anal. Biochem. 102, 344 (1980), (7) S.A. Latt et al.: J. Histochem. Cytochem. 24, 24 (1976)
InChi	InChI=1S/C25H24N6O/c1-30-10-12-31(13-11-30)18-5-9-21-23(15-18)29-25(27-21)17-4-8-20-22(14-17)28-24(26-20)16-2-6-19(32)7-3-16/h2-9,14-15,32H,10-13H2,1H3,(H,26,28)(H,27,29)
Quantity	500 mg, 5 g, Bulk