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    Hoechst 33258

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    Hoechst 33258

    SKU
    B0029

    Category: Fluorescent Detection

    Synonyms
    4-[5-(4-Methyl-1-piperazinyl)-1H,1'H-2,5'-bibenzimidazol-2'-yl]phenol trihydrochloride
    23491-45-4
    CAS-Number
    C25H24N6O . 3HCl
    Molecular Formula
    533.88
    Molecular Weight

    • Product Data
    • Handling
    • Safety Information
    • Details

    Specifications

    Purity
    ≥98% (HPLC, TLC)
    Identity
    1H-NMR
    Appearance
    Yellow solid

    Properties

    Fluorescence
    λex 355 nm, λem 465 nm in TE buffer
    Melting Point
    >300 °C (lit.)
    Solvents
    water, ethanol, DMSO

    Downloads

    Safety Data Sheet
    CDX B0029 MSDS.pdf
    Long Term Storage
    +4°C
    Use / Stability
    Stable for at least 2 years after receipt when stored at +4°C.
    Handling Advice
    Protect from light and moisture.
    Short Term Storage
    +4°C
    Shipping
    AMBIENT
    Transportation
    Not dangerous goods
    Signal word
    Warning
    GHS Symbol
    GHS07
    Precautionary statements
    P305 + P351 + P338
    Hazard statements
    H302, H315, H319
    Description
    Useful for staining DNA, chromosomes and nuclei. Binds to the minor groove of DNA at AT-rich sequences. This dye is commonly used for determining the DNA content of viable cells without detergent treatment or fixation and for fluorescence microscopy or flow cytometry.
    InChi
    InChI=1S/C25H24N6O/c1-30-10-12-31(13-11-30)18-5-9-21-23(15-18)29-25(27-21)17-4-8-20-22(14-17)28-24(26-20)16-2-6-19(32)7-3-16/h2-9,14-15,32H,10-13H2,1H3,(H,26,28)(H,27,29)
    References
    (1) A. Kumar et al.: In Vitro Cell. & Developm. Biol. 44(7), 189 (2008) , (2) D. Weidmann et al.: Meth. Enzym. 446, 277 (2008) , (3) D. Plesca et al.: Meth. Enzym. 446, 107 (2008) , (4) K.H. Elstein et al.: Exp. Cell. Res. 211, 322 (1994) , (5) Y.-J. Kim et al.: Anal. Biochem. 174, 168 (1988) , (6) C. Labarca et al.: Anal. Biochem. 102, 344 (1980) , (7) S.A. Latt et al.: J. Histochem. Cytochem. 24, 24 (1976)
    InChi Key
    INAAIJLSXJJHOZ-UHFFFAOYSA-N
    Smiles
    CN1CCN(CC1)C1=CC=C2NC(=NC2=C1)C1=CC=C2NC(=NC2=C1)C1=CC=C(O)C=C1

    Useful for staining DNA, chromosomes and nuclei. Binds to the minor groove of DNA at AT-rich sequences. This dye is commonly used for determining the DNA content of viable cells without detergent treatment or fixation and for fluorescence microscopy or flow cytometry.

    SKU: B0029 Category: Fluorescent Detection
    • Additional information

    Additional information

    Synonyms

    4-[5-(4-Methyl-1-piperazinyl)-1H,1'H-2,5'-bibenzimidazol-2'-yl]phenol trihydrochloride

    Purity

    ≥98% (HPLC, TLC)

    Appearance

    Yellow solid

    CAS-Number

    23491-45-4

    Molecular Formula

    C25H24N6O . 3HCl

    Molecular Weight

    533.88

    Identity

    1H-NMR

    Solvents

    water, ethanol, DMSO

    Melting Point

    >300 °C (lit.)

    Fluorescence

    λex 355 nm, λem 465 nm in TE buffer

    Smiles

    CN1CCN(CC1)C1=CC=C2NC(=NC2=C1)C1=CC=C2NC(=NC2=C1)C1=CC=C(O)C=C1

    InChi Key

    INAAIJLSXJJHOZ-UHFFFAOYSA-N

    Shipping

    AMBIENT

    Short Term Storage

    +4°C

    Long Term Storage

    +4°C

    Handling Advice

    Protect from light and moisture.

    Use / Stability

    Stable for at least 2 years after receipt when stored at +4°C.

    Hazard statements

    H302, H315, H319

    Precautionary statements

    P305 + P351 + P338

    GHS Symbol

    GHS07

    Signal word

    Warning

    Transportation

    Not dangerous goods

    References

    (1) A. Kumar et al.: In Vitro Cell. & Developm. Biol. 44(7), 189 (2008), (2) D. Weidmann et al.: Meth. Enzym. 446, 277 (2008), (3) D. Plesca et al.: Meth. Enzym. 446, 107 (2008), (4) K.H. Elstein et al.: Exp. Cell. Res. 211, 322 (1994), (5) Y.-J. Kim et al.: Anal. Biochem. 174, 168 (1988), (6) C. Labarca et al.: Anal. Biochem. 102, 344 (1980), (7) S.A. Latt et al.: J. Histochem. Cytochem. 24, 24 (1976)

    InChi

    InChI=1S/C25H24N6O/c1-30-10-12-31(13-11-30)18-5-9-21-23(15-18)29-25(27-21)17-4-8-20-22(14-17)28-24(26-20)16-2-6-19(32)7-3-16/h2-9,14-15,32H,10-13H2,1H3,(H,26,28)(H,27,29)

    Quantity

    500 mg, 5 g, Bulk

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