- Synonyms: 4-[5-(4-Methyl-1-piperazinyl)-1H,1'H-2,5'-bibenzimidazol-2'-yl]phenol trihydrochloride
- Purity: ≥98% (HPLC, TLC)
- Appearance: Yellow solid.
- CAS-Number: 23491-45-4
- Molecular Formula: C25H24N6O . 3HCl
- Molecular Weight: 533.88
- Identity: 1H-NMR
- Solvents: water, ethanol, DMSO
- Melting Point: >300 °C (lit.)
- Fluorescence: λex 355 nm; λem 465 nm in TE buffer
- Smiles: CN1CCN(CC1)C1=CC=C2NC(=NC2=C1)C1=CC=C2NC(=NC2=C1)C1=CC=C(O)C=C1
- InChi: InChI=1S/C25H24N6O/c1-30-10-12-31(13-11-30)18-5-9-21-23(15-18)29-25(27-21)17-4-8-20-22(14-17)28-24(26-20)16-2-6-19(32)7-3-16/h2-9,14-15,32H,10-13H2,1H3,(H,26,28)(H,27,29)
- InChi Key: INAAIJLSXJJHOZ-UHFFFAOYSA-N
Available from stock
Hoechst 33258
SKU: B0029
Category: Fluorescent Detection
- Shipping: AMBIENT
- Short Term Storage: +4°C
- Long Term Storage: +4°C
- Handling Advice: Protect from light and moisture.
- Use / Stability: Stable for at least 2 years after receipt when stored at +4°C.
- Description: Useful for staining DNA, chromosomes and nuclei. Binds to the minor groove of DNA at AT-rich sequences. This dye is commonly used for determining the DNA content of viable cells without detergent treatment or fixation and for fluorescence microscopy or flow cytometry.
- References: (1) A. Kumar et al.; In Vitro Cell. & Developm. Biol. 44(7), 189 (2008), (2) D. Weidmann et al.; Meth. Enzym. 446, 277 (2008), (3) D. Plesca et al.; Meth. Enzym. 446, 107 (2008), (4) K.H. Elstein et al.; Exp. Cell. Res. 211, 322 (1994), (5) Y.-J. Kim et al.; Anal. Biochem. 174, 168 (1988), (6) C. Labarca et al.; Anal. Biochem. 102, 344 (1980), (7) S.A. Latt et al.; J. Histochem. Cytochem. 24, 24 (1976)
Useful for staining DNA, chromosomes and nuclei. Binds to the minor groove of DNA at AT-rich sequences. This dye is commonly used for determining the DNA content of viable cells without detergent treatment or fixation and for fluorescence microscopy or flow cytometry.
Additional information
| Melting Point | >300 °C (lit.) |
|---|---|
| Handling Advice | Protect from light and moisture. |
| Identity | 1H-NMR |
| Availability | 1 |
| CAS-Number | 23491-45-4 |
| Fluorescence | λex 355 nm; λem 465 nm in TE buffer |
| InChi | InChI=1S/C25H24N6O/c1-30-10-12-31(13-11-30)18-5-9-21-23(15-18)29-25(27-21)17-4-8-20-22(14-17)28-24(26-20)16-2-6-19(32)7-3-16/h2-9,14-15,32H,10-13H2,1H3,(H,26,28)(H,27,29) |
| Appearance | Yellow solid. |
| InChi Key | INAAIJLSXJJHOZ-UHFFFAOYSA-N |
| Long Term Storage | +4°C |
| Quantity | 5 g, 500 mg, Bulk |
| Pricetable | |
| Molecular Formula | |
| References | (1) A. Kumar et al.; In Vitro Cell. & Developm. Biol. 44(7), 189 (2008), (2) D. Weidmann et al.; Meth. Enzym. 446, 277 (2008), (3) D. Plesca et al.; Meth. Enzym. 446, 107 (2008), (4) K.H. Elstein et al.; Exp. Cell. Res. 211, 322 (1994), (5) Y.-J. Kim et al.; Anal. Biochem. 174, 168 (1988), (6) C. Labarca et al.; Anal. Biochem. 102, 344 (1980), (7) S.A. Latt et al.; J. Histochem. Cytochem. 24, 24 (1976) |
| Shipping | AMBIENT |
| Use / Stability | Stable for at least 2 years after receipt when stored at +4°C. |
| Purity | |
| Short Term Storage | +4°C |
| Molecular Weight | |
| Solvents | water, ethanol, DMSO |
| Synonyms | 4-[5-(4-Methyl-1-piperazinyl)-1H,1'H-2,5'-bibenzimidazol-2'-yl]phenol trihydrochloride |
| Smiles | CN1CCN(CC1)C1=CC=C2NC(=NC2=C1)C1=CC=C2NC(=NC2=C1)C1=CC=C(O)C=C1 |
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