Specifications
- Purity
- ≥98% (HPLC, TLC)
- Identity
- 1H-NMR
- Appearance
- Yellow solid
Properties
- Fluorescence
- λex 355 nm, λem 465 nm in TE buffer
- Melting Point
- >300 °C (lit.)
- Solvents
- water, ethanol, DMSO
Downloads
- Safety Data Sheet
- CDX B0029 MSDS.pdf
Category: Fluorescent Detection
Useful for staining DNA, chromosomes and nuclei. Binds to the minor groove of DNA at AT-rich sequences. This dye is commonly used for determining the DNA content of viable cells without detergent treatment or fixation and for fluorescence microscopy or flow cytometry.
Synonyms | 4-[5-(4-Methyl-1-piperazinyl)-1H,1'H-2,5'-bibenzimidazol-2'-yl]phenol trihydrochloride |
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Purity | ≥98% (HPLC, TLC) |
Appearance | Yellow solid |
CAS-Number | 23491-45-4 |
Molecular Formula | C25H24N6O . 3HCl |
Molecular Weight | 533.88 |
Identity | 1H-NMR |
Solvents | water, ethanol, DMSO |
Melting Point | >300 °C (lit.) |
Fluorescence | λex 355 nm, λem 465 nm in TE buffer |
Smiles | CN1CCN(CC1)C1=CC=C2NC(=NC2=C1)C1=CC=C2NC(=NC2=C1)C1=CC=C(O)C=C1 |
InChi Key | INAAIJLSXJJHOZ-UHFFFAOYSA-N |
Shipping | AMBIENT |
Short Term Storage | +4°C |
Long Term Storage | +4°C |
Handling Advice | Protect from light and moisture. |
Use / Stability | Stable for at least 2 years after receipt when stored at +4°C. |
Hazard statements | H302, H315, H319 |
Precautionary statements | P305 + P351 + P338 |
GHS Symbol | GHS07 |
Signal word | Warning |
Transportation | Not dangerous goods |
References | (1) A. Kumar et al.: In Vitro Cell. & Developm. Biol. 44(7), 189 (2008), (2) D. Weidmann et al.: Meth. Enzym. 446, 277 (2008), (3) D. Plesca et al.: Meth. Enzym. 446, 107 (2008), (4) K.H. Elstein et al.: Exp. Cell. Res. 211, 322 (1994), (5) Y.-J. Kim et al.: Anal. Biochem. 174, 168 (1988), (6) C. Labarca et al.: Anal. Biochem. 102, 344 (1980), (7) S.A. Latt et al.: J. Histochem. Cytochem. 24, 24 (1976) |
InChi | InChI=1S/C25H24N6O/c1-30-10-12-31(13-11-30)18-5-9-21-23(15-18)29-25(27-21)17-4-8-20-22(14-17)28-24(26-20)16-2-6-19(32)7-3-16/h2-9,14-15,32H,10-13H2,1H3,(H,26,28)(H,27,29) |
Quantity | 500 mg, 5 g, Bulk |
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