Specifications
- Purity
- ≥98% (HPLC, TLC)
- Identity
- 1H-NMR
- Appearance
- Yellow solid
Properties
- Fluorescence
- λex 355 nm, λem 465 nm in TE buffer
- Melting Point
- >300 °C (lit.)
- Solvents
- water, ethanol, DMSO
Downloads
- Safety Data Sheet
- CDX B0029 MSDS.pdf
Available from stock
Hoechst 33258
- SKU
- B0029
Category: Fluorescent Detection
- Synonyms
- 4-[5-(4-Methyl-1-piperazinyl)-1H,1'H-2,5'-bibenzimidazol-2'-yl]phenol trihydrochloride
- 23491-45-4
- CAS-Number
- C25H24N6O . 3HCl
- Molecular Formula
- 533.88
- Molecular Weight
- Long Term Storage
- +4°C
- Use / Stability
- Stable for at least 2 years after receipt when stored at +4°C.
- Handling Advice
- Protect from light and moisture.
- Short Term Storage
- +4°C
- Shipping
- AMBIENT
- Transportation
- Not dangerous goods
- Signal word
- Warning
- GHS Symbol
- GHS07
- Precautionary statements
- P305 + P351 + P338
- Hazard statements
- H302, H315, H319
- Description
- Useful for staining DNA, chromosomes and nuclei. Binds to the minor groove of DNA at AT-rich sequences. This dye is commonly used for determining the DNA content of viable cells without detergent treatment or fixation and for fluorescence microscopy or flow cytometry.
- InChi
- InChI=1S/C25H24N6O/c1-30-10-12-31(13-11-30)18-5-9-21-23(15-18)29-25(27-21)17-4-8-20-22(14-17)28-24(26-20)16-2-6-19(32)7-3-16/h2-9,14-15,32H,10-13H2,1H3,(H,26,28)(H,27,29)
- References
- (1) A. Kumar et al.: In Vitro Cell. & Developm. Biol. 44(7), 189 (2008) , (2) D. Weidmann et al.: Meth. Enzym. 446, 277 (2008) , (3) D. Plesca et al.: Meth. Enzym. 446, 107 (2008) , (4) K.H. Elstein et al.: Exp. Cell. Res. 211, 322 (1994) , (5) Y.-J. Kim et al.: Anal. Biochem. 174, 168 (1988) , (6) C. Labarca et al.: Anal. Biochem. 102, 344 (1980) , (7) S.A. Latt et al.: J. Histochem. Cytochem. 24, 24 (1976)
- InChi Key
- INAAIJLSXJJHOZ-UHFFFAOYSA-N
- Smiles
- CN1CCN(CC1)C1=CC=C2NC(=NC2=C1)C1=CC=C2NC(=NC2=C1)C1=CC=C(O)C=C1
Useful for staining DNA, chromosomes and nuclei. Binds to the minor groove of DNA at AT-rich sequences. This dye is commonly used for determining the DNA content of viable cells without detergent treatment or fixation and for fluorescence microscopy or flow cytometry.
Additional information
| Synonyms | 4-[5-(4-Methyl-1-piperazinyl)-1H,1'H-2,5'-bibenzimidazol-2'-yl]phenol trihydrochloride |
|---|---|
| Purity | ≥98% (HPLC, TLC) |
| Appearance | Yellow solid |
| CAS-Number | 23491-45-4 |
| Molecular Formula | C25H24N6O . 3HCl |
| Molecular Weight | 533.88 |
| Identity | 1H-NMR |
| Solvents | water, ethanol, DMSO |
| Melting Point | >300 °C (lit.) |
| Fluorescence | λex 355 nm, λem 465 nm in TE buffer |
| Smiles | CN1CCN(CC1)C1=CC=C2NC(=NC2=C1)C1=CC=C2NC(=NC2=C1)C1=CC=C(O)C=C1 |
| InChi Key | INAAIJLSXJJHOZ-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | +4°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at +4°C. |
| Hazard statements | H302, H315, H319 |
| Precautionary statements | P305 + P351 + P338 |
| GHS Symbol | GHS07 |
| Signal word | Warning |
| Transportation | Not dangerous goods |
| References | (1) A. Kumar et al.: In Vitro Cell. & Developm. Biol. 44(7), 189 (2008), (2) D. Weidmann et al.: Meth. Enzym. 446, 277 (2008), (3) D. Plesca et al.: Meth. Enzym. 446, 107 (2008), (4) K.H. Elstein et al.: Exp. Cell. Res. 211, 322 (1994), (5) Y.-J. Kim et al.: Anal. Biochem. 174, 168 (1988), (6) C. Labarca et al.: Anal. Biochem. 102, 344 (1980), (7) S.A. Latt et al.: J. Histochem. Cytochem. 24, 24 (1976) |
| InChi | InChI=1S/C25H24N6O/c1-30-10-12-31(13-11-30)18-5-9-21-23(15-18)29-25(27-21)17-4-8-20-22(14-17)28-24(26-20)16-2-6-19(32)7-3-16/h2-9,14-15,32H,10-13H2,1H3,(H,26,28)(H,27,29) |
| Quantity | 500 mg, 5 g, Bulk |
