Hoechst 33258

SKU: B0029

Category: Fluorescent Detection

Synonyms: 4-[5-(4-Methyl-1-piperazinyl)-1H,1'H-2,5'-bibenzimidazol-2'-yl]phenol trihydrochloride; 23491-45-4
CAS-Number: C₂₅H₂₄N₆O . ₃HCl
Molecular Formula: 533.88
Molecular Weight

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥98% (HPLC, TLC)
Identity: 1H-NMR
Appearance: Yellow solid

Properties

Fluorescence: λex 355 nm, λem 465 nm in TE buffer
Melting Point: >300 °C (lit.)
Solvents: water, ethanol, DMSO

Long Term Storage: +4°C
Use / Stability: Stable for at least 2 years after receipt when stored at +4°C.
Handling Advice: Protect from light and moisture.
Short Term Storage: +4°C
Shipping: AMBIENT

Transportation: Not dangerous goods
Signal word: Warning
GHS Symbol: GHS07
Precautionary statements: P305 + P351 + P338
Hazard statements: H302, H315, H319

Description: Useful for staining DNA, chromosomes and nuclei. Binds to the minor groove of DNA at AT-rich sequences. This dye is commonly used for determining the DNA content of viable cells without detergent treatment or fixation and for fluorescence microscopy or flow cytometry.
InChi: InChI=1S/C25H24N6O/c1-30-10-12-31(13-11-30)18-5-9-21-23(15-18)29-25(27-21)17-4-8-20-22(14-17)28-24(26-20)16-2-6-19(32)7-3-16/h2-9,14-15,32H,10-13H2,1H3,(H,26,28)(H,27,29)
References: (1) A. Kumar et al.: In Vitro Cell. & Developm. Biol. 44(7), 189 (2008) , (2) D. Weidmann et al.: Meth. Enzym. 446, 277 (2008) , (3) D. Plesca et al.: Meth. Enzym. 446, 107 (2008) , (4) K.H. Elstein et al.: Exp. Cell. Res. 211, 322 (1994) , (5) Y.-J. Kim et al.: Anal. Biochem. 174, 168 (1988) , (6) C. Labarca et al.: Anal. Biochem. 102, 344 (1980) , (7) S.A. Latt et al.: J. Histochem. Cytochem. 24, 24 (1976)
InChi Key: INAAIJLSXJJHOZ-UHFFFAOYSA-N
Smiles: CN1CCN(CC1)C1=CC=C2NC(=NC2=C1)C1=CC=C2NC(=NC2=C1)C1=CC=C(O)C=C1

Useful for staining DNA, chromosomes and nuclei. Binds to the minor groove of DNA at AT-rich sequences. This dye is commonly used for determining the DNA content of viable cells without detergent treatment or fixation and for fluorescence microscopy or flow cytometry.

SKU: B0029 Category: Fluorescent Detection

Additional information

Additional information

Synonyms	4-[5-(4-Methyl-1-piperazinyl)-1H,1'H-2,5'-bibenzimidazol-2'-yl]phenol trihydrochloride
Purity	≥98% (HPLC, TLC)
Appearance	Yellow solid
CAS-Number	23491-45-4
Molecular Formula	C25H24N6O . 3HCl
Molecular Weight	533.88
Identity	1H-NMR
Solvents	water, ethanol, DMSO
Melting Point	>300 °C (lit.)
Fluorescence	λex 355 nm, λem 465 nm in TE buffer
Smiles	CN1CCN(CC1)C1=CC=C2NC(=NC2=C1)C1=CC=C2NC(=NC2=C1)C1=CC=C(O)C=C1
InChi Key	INAAIJLSXJJHOZ-UHFFFAOYSA-N
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	+4°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at +4°C.
Hazard statements	H302, H315, H319
Precautionary statements	P305 + P351 + P338
GHS Symbol	GHS07
Signal word	Warning
Transportation	Not dangerous goods
References	(1) A. Kumar et al.: In Vitro Cell. & Developm. Biol. 44(7), 189 (2008), (2) D. Weidmann et al.: Meth. Enzym. 446, 277 (2008), (3) D. Plesca et al.: Meth. Enzym. 446, 107 (2008), (4) K.H. Elstein et al.: Exp. Cell. Res. 211, 322 (1994), (5) Y.-J. Kim et al.: Anal. Biochem. 174, 168 (1988), (6) C. Labarca et al.: Anal. Biochem. 102, 344 (1980), (7) S.A. Latt et al.: J. Histochem. Cytochem. 24, 24 (1976)
InChi	InChI=1S/C25H24N6O/c1-30-10-12-31(13-11-30)18-5-9-21-23(15-18)29-25(27-21)17-4-8-20-22(14-17)28-24(26-20)16-2-6-19(32)7-3-16/h2-9,14-15,32H,10-13H2,1H3,(H,26,28)(H,27,29)
Quantity	500 mg, 5 g, Bulk