Specifications
- Purity
- ≥98% (HPLC)
- Appearance
- Light yellow powder
- Identity
- 1H-NMR
Properties
- Solvents
- DMSO
- Fluorescence
- λex 500 nm, λem 515 nm in DMSO
Documentation
- Safety Data Sheet (SDS)
- CDX A0024 MSDS_Vs1.pdf
Available from stock
DAF-FM DA
- SKU
- A0024
Categories: Fluorescent Detection, Fluorescent Detection
- Synonyms
- 3-Amino-4-(N-methylamino)-2', 7'-difluorofluorescein diacetate
- 254109-22-3
- CAS-Number
- C25H18F2N2O7
- Molecular Formula
- 496.42
- Molecular Weight
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
- Shipping
- AMBIENT
- Short Term Storage
- +4°C
- Long Term Storage
- -20°C
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at -20°C.
- Transportation
- Not dangerous goods
- Description
- DAF-FM diacetate is an important reagent for quantification of low concentrations of nitric oxide in solution. This compound is essentially nonfluorescent until it reacts with NO to form a fluorescent benzotriazole. With excitation/emission maxima of 495/515 nm, DAF-FM can be detected using any instrument suitable for detecting fluorescein.
- Smiles
- CNC1=C(N)C2=C(C=C1)C1(OC2=O)C2=C(OC3=C1C=C(F)C(OC(C)=O)=C3)C=C(OC(C)=O)C(F)=C2
- InChi Key
- BEVHTVRRVVEMEF-UHFFFAOYSA-N
- References
- (1) J. S. Carroll et al.: Anal. Chem. 79(14), 5133 (2007) , (2) Y. Itoh et al.: Anal. Biochem. 287(2), 203 (2000) , (3) H. Kojima et al.: Angew. Chem. 111(219), 3419 (1999)
- InChi
- InChI=1S/C25H18F2N2O7/c1-10(30)33-20-8-18-13(6-15(20)26)25(12-4-5-17(29-3)23(28)22(12)24(32)36-25)14-7-16(27)21(34-11(2)31)9-19(14)35-18/h4-9,29H,28H2,1-3H3
DAF-FM diacetate is an important reagent for quantification of low concentrations of nitric oxide in solution. This compound is essentially nonfluorescent until it reacts with NO to form a fluorescent benzotriazole. With excitation/emission maxima of 495/515 nm, DAF-FM can be detected using any instrument suitable for detecting fluorescein.
Additional information
| Synonyms | 3-Amino-4-(N-methylamino)-2', 7'-difluorofluorescein diacetate |
|---|---|
| Purity | ≥98% (HPLC) |
| Appearance | Light yellow powder |
| CAS-Number | 254109-22-3 |
| Molecular Formula | C25H18F2N2O7 |
| Molecular Weight | 496.42 |
| Identity | 1H-NMR |
| Solvents | DMSO |
| Fluorescence | λex 500 nm, λem 515 nm in DMSO |
| Smiles | CNC1=C(N)C2=C(C=C1)C1(OC2=O)C2=C(OC3=C1C=C(F)C(OC(C)=O)=C3)C=C(OC(C)=O)C(F)=C2 |
| InChi Key | BEVHTVRRVVEMEF-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Transportation | Not dangerous goods |
| References | (1) J. S. Carroll et al.: Anal. Chem. 79(14), 5133 (2007), (2) Y. Itoh et al.: Anal. Biochem. 287(2), 203 (2000), (3) H. Kojima et al.: Angew. Chem. 111(219), 3419 (1999) |
| InChi | InChI=1S/C25H18F2N2O7/c1-10(30)33-20-8-18-13(6-15(20)26)25(12-4-5-17(29-3)23(28)22(12)24(32)36-25)14-7-16(27)21(34-11(2)31)9-19(14)35-18/h4-9,29H,28H2,1-3H3 |
| Quantity | 1 mg, 5 mg, Bulk |
