DAF-FM solution (5 mM in DMSO), 1 mg in 0.49 ml DMSO

SKU: A0523

Category: Fluorescent Detection

Synonyms: 4-Amino-5-methylamino- 2',7'-difluorofluorescein , Diaminofluorescein-FM; 254109-20-1
CAS-Number: C₂₁H₁₄F₂N₂O₅
Molecular Formula: 412.34
Molecular Weight

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥98% (HPLC)
Identity: 1H-NMR
Appearance: Clear colorless to yellow liquid

Properties

Density: 1.100 g/cm3
Solvents: DMSO

Short Term Storage: +4°C
Use / Stability: Stable for at least 2 years after receipt when stored at -20°C.
Handling Advice: Protect from light and moisture.
Long Term Storage: -20°C
Shipping: AMBIENT

Transportation: Packing Group III
RIDADR: UN1993

Description: DAF-FM is a highly sensitive, photo-stable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM is more sensitive to nitric oxide (NO) compared with DAF-2. DAF-FM (Ex: 500 nm, Em: 515 nm, dectection limit: 3 nM) is more photo-stable and less pH sensitive than DAF-2. DAF-FM is not quite as cell-permeable as the diacetate derivative, but it does not need to be activated by cytosolic enzymes.
InChi: InChI=1S/C21H14F2N2O5/c1-25-13-3-2-8(19(20(13)24)21(28)29)18-9-4-11(22)14(26)6-16(9)30-17-7-15(27)12(23)5-10(17)18/h2-7,25-26H,24H2,1H3,(H,28,29)
References: (1) W.S. Kim et al., Anal. Chem. 78(6), 1859 (2006) , (2) M.M. Knight et al., Am. J. Physiol. 284(4, Pt. 1), C1083 (2003) , (3) H. Kojima et al., Adv. Mat. 12(10), 763 (2002) , (4) H. Kojima et al., Angew. Chem. 111( 21), 3419 (1999)
InChi Key: BJTLSPOVXMBXRZ-UHFFFAOYSA-N
Smiles: O=C1C=C2OC3=C(C(C4=C(C(O)=O)C(N)=C(NC)C=C4)=C2C=C1F)C=C(F)C(O)=C3

DAF-FM is a highly sensitive, photo-stable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM is more sensitive to nitric oxide (NO) compared with DAF-2. DAF-FM (Ex: 500 nm, Em: 515 nm, dectection limit: 3 nM) is more photo-stable and less pH sensitive than DAF-2. DAF-FM is not quite as cell-permeable as the diacetate derivative, but it does not need to be activated by cytosolic enzymes.

SKU: A0523 Category: Fluorescent Detection

Additional information

Additional information

Synonyms	4-Amino-5-methylamino- 2',7'-difluorofluorescein, Diaminofluorescein-FM
Purity	≥98% (HPLC)
Appearance	Clear colorless to yellow liquid
CAS-Number	254109-20-1
Molecular Formula	C21H14F2N2O5
Molecular Weight	412.34
Identity	1H-NMR
Solvents	DMSO
Density	1.100 g/cm3
Smiles	O=C1C=C2OC3=C(C(C4=C(C(O)=O)C(N)=C(NC)C=C4)=C2C=C1F)C=C(F)C(O)=C3
InChi Key	BJTLSPOVXMBXRZ-UHFFFAOYSA-N
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at -20°C.
RIDADR	UN1993
Transportation	Packing Group III
References	(1) W.S. Kim et al., Anal. Chem. 78(6), 1859 (2006), (2) M.M. Knight et al., Am. J. Physiol. 284(4, Pt. 1), C1083 (2003), (3) H. Kojima et al., Adv. Mat. 12(10), 763 (2002), (4) H. Kojima et al., Angew. Chem. 111( 21), 3419 (1999)
InChi	InChI=1S/C21H14F2N2O5/c1-25-13-3-2-8(19(20(13)24)21(28)29)18-9-4-11(22)14(26)6-16(9)30-17-7-15(27)12(23)5-10(17)18/h2-7,25-26H,24H2,1H3,(H,28,29)
Quantity	1 mg, Bulk