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    DAF-FM solution (5 mM in DMSO)

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      DAF-FM solution (5 mM in DMSO), 1 mg in 0.49 ml DMSO

      SKU
      A0523

      Categories: Fluorescent Detection, Fluorescent Detection

      Synonyms
      4-Amino-5-methylamino- 2',7'-difluorofluorescein , Diaminofluorescein-FM
      254109-20-1
      CAS-Number
      C21H14F2N2O5
      Molecular Formula
      412.34
      Molecular Weight

      For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

      • Product Data
      • Handling
      • Safety Information
      • Details

      Specifications

      Purity
      ≥98% (HPLC)
      Appearance
      Clear colorless to yellow liquid
      Identity
      1H-NMR

      Properties

      Solvents
      DMSO
      Density
      1.100 g/cm3
      Shipping
      AMBIENT
      Short Term Storage
      +4°C
      Long Term Storage
      -20°C
      Handling Advice
      Protect from light and moisture.
      Use / Stability
      Stable for at least 2 years after receipt when stored at -20°C.
      RIDADR
      UN1993
      Transportation
      Packing Group III
      Description
      DAF-FM is a highly sensitive, photo-stable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM is more sensitive to nitric oxide (NO) compared with DAF-2. DAF-FM (Ex: 500 nm, Em: 515 nm, dectection limit: 3 nM) is more photo-stable and less pH sensitive than DAF-2. DAF-FM is not quite as cell-permeable as the diacetate derivative, but it does not need to be activated by cytosolic enzymes.
      Smiles
      O=C1C=C2OC3=C(C(C4=C(C(O)=O)C(N)=C(NC)C=C4)=C2C=C1F)C=C(F)C(O)=C3
      InChi Key
      BJTLSPOVXMBXRZ-UHFFFAOYSA-N
      References
      (1) W.S. Kim et al., Anal. Chem. 78(6), 1859 (2006) , (2) M.M. Knight et al., Am. J. Physiol. 284(4, Pt. 1), C1083 (2003) , (3) H. Kojima et al., Adv. Mat. 12(10), 763 (2002) , (4) H. Kojima et al., Angew. Chem. 111( 21), 3419 (1999)
      InChi
      InChI=1S/C21H14F2N2O5/c1-25-13-3-2-8(19(20(13)24)21(28)29)18-9-4-11(22)14(26)6-16(9)30-17-7-15(27)12(23)5-10(17)18/h2-7,25-26H,24H2,1H3,(H,28,29)

      DAF-FM is a highly sensitive, photo-stable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM is more sensitive to nitric oxide (NO) compared with DAF-2. DAF-FM (Ex: 500 nm, Em: 515 nm, dectection limit: 3 nM) is more photo-stable and less pH sensitive than DAF-2. DAF-FM is not quite as cell-permeable as the diacetate derivative, but it does not need to be activated by cytosolic enzymes.

      SKU: A0523 Categories: Fluorescent Detection, Fluorescent Detection
      • Additional information

      Additional information

      Synonyms

      4-Amino-5-methylamino- 2',7'-difluorofluorescein, Diaminofluorescein-FM

      Purity

      ≥98% (HPLC)

      Appearance

      Clear colorless to yellow liquid

      CAS-Number

      254109-20-1

      Molecular Formula

      C21H14F2N2O5

      Molecular Weight

      412.34

      Identity

      1H-NMR

      Solvents

      DMSO

      Density

      1.100 g/cm3

      Smiles

      O=C1C=C2OC3=C(C(C4=C(C(O)=O)C(N)=C(NC)C=C4)=C2C=C1F)C=C(F)C(O)=C3

      InChi Key

      BJTLSPOVXMBXRZ-UHFFFAOYSA-N

      Shipping

      AMBIENT

      Short Term Storage

      +4°C

      Long Term Storage

      -20°C

      Handling Advice

      Protect from light and moisture.

      Use / Stability

      Stable for at least 2 years after receipt when stored at -20°C.

      RIDADR

      UN1993

      Transportation

      Packing Group III

      References

      (1) W.S. Kim et al., Anal. Chem. 78(6), 1859 (2006), (2) M.M. Knight et al., Am. J. Physiol. 284(4, Pt. 1), C1083 (2003), (3) H. Kojima et al., Adv. Mat. 12(10), 763 (2002), (4) H. Kojima et al., Angew. Chem. 111( 21), 3419 (1999)

      InChi

      InChI=1S/C21H14F2N2O5/c1-25-13-3-2-8(19(20(13)24)21(28)29)18-9-4-11(22)14(26)6-16(9)30-17-7-15(27)12(23)5-10(17)18/h2-7,25-26H,24H2,1H3,(H,28,29)

      Quantity

      1 mg, Bulk

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