Specifications
- Purity
- ≥98% (HPLC)
- Appearance
- Clear colorless to yellow liquid
- Identity
- 1H-NMR
Properties
- Solvents
- DMSO
- Density
- 1.100 g/cm3
Documentation
- Safety Data Sheet (SDS)
- CDX A0523 SDS_Vs1.1.pdf
Available from stock
DAF-FM solution (5 mM in DMSO), 1 mg in 0.49 ml DMSO
- SKU
- A0523
Category: Fluorescent Detection
- Synonyms
- 4-Amino-5-methylamino- 2',7'-difluorofluorescein , Diaminofluorescein-FM
- 254109-20-1
- CAS-Number
- C21H14F2N2O5
- Molecular Formula
- 412.34
- Molecular Weight
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
- Shipping
- AMBIENT
- Short Term Storage
- +4°C
- Long Term Storage
- -20°C
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- Stable for at least 2 years after receipt when stored at -20°C.
- Transportation
- Not dangerous goods
- Description
- DAF-FM is a highly sensitive, photo-stable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM is more sensitive to nitric oxide (NO) compared with DAF-2. DAF-FM (Ex: 500 nm, Em: 515 nm, dectection limit: 3 nM) is more photo-stable and less pH sensitive than DAF-2. DAF-FM is not quite as cell-permeable as the diacetate derivative, but it does not need to be activated by cytosolic enzymes.
- Smiles
- O=C1C=C2OC3=C(C(C4=C(C(O)=O)C(N)=C(NC)C=C4)=C2C=C1F)C=C(F)C(O)=C3
- InChi Key
- BJTLSPOVXMBXRZ-UHFFFAOYSA-N
- References
- (1) W.S. Kim et al., Anal. Chem. 78(6), 1859 (2006) , (2) M.M. Knight et al., Am. J. Physiol. 284(4, Pt. 1), C1083 (2003) , (3) H. Kojima et al., Adv. Mat. 12(10), 763 (2002) , (4) H. Kojima et al., Angew. Chem. 111( 21), 3419 (1999)
- InChi
- InChI=1S/C21H14F2N2O5/c1-25-13-3-2-8(19(20(13)24)21(28)29)18-9-4-11(22)14(26)6-16(9)30-17-7-15(27)12(23)5-10(17)18/h2-7,25-26H,24H2,1H3,(H,28,29)
DAF-FM is a highly sensitive, photo-stable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM is more sensitive to nitric oxide (NO) compared with DAF-2. DAF-FM (Ex: 500 nm, Em: 515 nm, dectection limit: 3 nM) is more photo-stable and less pH sensitive than DAF-2. DAF-FM is not quite as cell-permeable as the diacetate derivative, but it does not need to be activated by cytosolic enzymes.
Additional information
| Synonyms | 4-Amino-5-methylamino- 2',7'-difluorofluorescein, Diaminofluorescein-FM |
|---|---|
| Purity | ≥98% (HPLC) |
| Appearance | Clear colorless to yellow liquid |
| CAS-Number | 254109-20-1 |
| Molecular Formula | C21H14F2N2O5 |
| Molecular Weight | 412.34 |
| Identity | 1H-NMR |
| Solvents | DMSO |
| Density | 1.100 g/cm3 |
| Smiles | O=C1C=C2OC3=C(C(C4=C(C(O)=O)C(N)=C(NC)C=C4)=C2C=C1F)C=C(F)C(O)=C3 |
| InChi Key | BJTLSPOVXMBXRZ-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Transportation | Not dangerous goods |
| References | (1) W.S. Kim et al., Anal. Chem. 78(6), 1859 (2006), (2) M.M. Knight et al., Am. J. Physiol. 284(4, Pt. 1), C1083 (2003), (3) H. Kojima et al., Adv. Mat. 12(10), 763 (2002), (4) H. Kojima et al., Angew. Chem. 111( 21), 3419 (1999) |
| InChi | InChI=1S/C21H14F2N2O5/c1-25-13-3-2-8(19(20(13)24)21(28)29)18-9-4-11(22)14(26)6-16(9)30-17-7-15(27)12(23)5-10(17)18/h2-7,25-26H,24H2,1H3,(H,28,29) |
| Quantity | 1 mg, Bulk |
