Specifications
- Purity
- ≥98% (HPLC)
- Appearance
- Orange powder
- Identity
- 1H-NMR
Properties
- Solvents
- DMSO
- Fluorescence
- λex 495 nm, λem 515 nm
Downloads
- Safety Data Sheet
- CDX A0023 MSDS.pdf
Categories: Fluorescent Detection, Fluorescent Detection
DAF-FM is an important reagent for quantitating low concentrations of nitric oxide in solution. This compound is essentially nonfluorescent until it reacts with NO to form a fluorescent benzotrizole. With excitation/emission maxima of 495/515 nm, DAF-FM can be detected using any instrument that can be used to detect fluorescein.
Synonyms | 3-Amino-4-(N-methylamino)-2', 7'-difluorofluorescein |
---|---|
Purity | ≥98% (HPLC) |
Appearance | Orange powder |
CAS-Number | 254109-20-1 |
Molecular Formula | C21H14F2N2O5 |
Molecular Weight | 412.34 |
Identity | 1H-NMR |
Solvents | DMSO |
Fluorescence | λex 495 nm, λem 515 nm |
Smiles | CNC1=C(N)C(C(O)=O)=C(C=C1)C1=C2C=C(F)C(=O)C=C2OC2=C1C=C(F)C(O)=C2 |
InChi Key | BJTLSPOVXMBXRZ-UHFFFAOYSA-N |
Shipping | AMBIENT |
Short Term Storage | +4°C |
Long Term Storage | -20°C |
Handling Advice | Protect from light and moisture. |
Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
Hazard statements | H302 |
GHS Symbol | GHS07 |
Signal word | Warning |
Transportation | Not dangerous goods |
References | (1) W.S. Kim et al.: Anal. Chem. 78(6), 1859 (2006), (2) M.M. Knight et al.: Am. J. Physiol. 284(4, Pt. 1), C1083 (2003), (3) H. Kojima et al.: Adv. Mat. 12(10), 763 (2002), (4) H. Kojima et al.: Angew. Chem. 111( 21), 3419 (1999) |
InChi | InChI=1S/C21H14F2N2O5/c1-25-13-3-2-8(19(20(13)24)21(28)29)18-9-4-11(22)14(26)6-16(9)30-17-7-15(27)12(23)5-10(17)18/h2-7,25-26H,24H2,1H3,(H,28,29) |
Quantity | 1 mg, 5 mg, Bulk |