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    DAF-FM

    Available from stock

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      DAF-FM

      SKU
      A0023

      Category: Fluorescent Detection

      Synonyms
      3-Amino-4-(N-methylamino)-2', 7'-difluorofluorescein
      254109-20-1
      CAS-Number
      C21H14F2N2O5
      Molecular Formula
      412.34
      Molecular Weight

      • Product Data
      • Handling
      • Safety Information
      • Details

      Specifications

      Purity
      ≥98% (HPLC)
      Identity
      1H-NMR
      Appearance
      Orange powder

      Properties

      Fluorescence
      λex 495 nm, λem 515 nm
      Solvents
      DMSO

      Downloads

      Safety Data Sheet
      CDX A0023 MSDS.pdf
      Long Term Storage
      -20°C
      Use / Stability
      Stable for at least 2 years after receipt when stored at -20°C.
      Handling Advice
      Protect from light and moisture.
      Short Term Storage
      +4°C
      Shipping
      AMBIENT
      Transportation
      Not dangerous goods
      Signal word
      Warning
      GHS Symbol
      GHS07
      Hazard statements
      H302
      Description
      DAF-FM is an important reagent for quantitating low concentrations of nitric oxide in solution. This compound is essentially nonfluorescent until it reacts with NO to form a fluorescent benzotrizole. With excitation/emission maxima of 495/515 nm, DAF-FM can be detected using any instrument that can be used to detect fluorescein.
      InChi
      InChI=1S/C21H14F2N2O5/c1-25-13-3-2-8(19(20(13)24)21(28)29)18-9-4-11(22)14(26)6-16(9)30-17-7-15(27)12(23)5-10(17)18/h2-7,25-26H,24H2,1H3,(H,28,29)
      References
      (1) W.S. Kim et al.: Anal. Chem. 78(6), 1859 (2006) , (2) M.M. Knight et al.: Am. J. Physiol. 284(4, Pt. 1), C1083 (2003) , (3) H. Kojima et al.: Adv. Mat. 12(10), 763 (2002) , (4) H. Kojima et al.: Angew. Chem. 111( 21), 3419 (1999)
      InChi Key
      BJTLSPOVXMBXRZ-UHFFFAOYSA-N
      Smiles
      CNC1=C(N)C(C(O)=O)=C(C=C1)C1=C2C=C(F)C(=O)C=C2OC2=C1C=C(F)C(O)=C2

      DAF-FM is an important reagent for quantitating low concentrations of nitric oxide in solution. This compound is essentially nonfluorescent until it reacts with NO to form a fluorescent benzotrizole. With excitation/emission maxima of 495/515 nm, DAF-FM can be detected using any instrument that can be used to detect fluorescein.

      SKU: A0023 Category: Fluorescent Detection
      • Additional information

      Additional information

      Synonyms

      3-Amino-4-(N-methylamino)-2', 7'-difluorofluorescein

      Purity

      ≥98% (HPLC)

      Appearance

      Orange powder

      CAS-Number

      254109-20-1

      Molecular Formula

      C21H14F2N2O5

      Molecular Weight

      412.34

      Identity

      1H-NMR

      Solvents

      DMSO

      Fluorescence

      λex 495 nm, λem 515 nm

      Smiles

      CNC1=C(N)C(C(O)=O)=C(C=C1)C1=C2C=C(F)C(=O)C=C2OC2=C1C=C(F)C(O)=C2

      InChi Key

      BJTLSPOVXMBXRZ-UHFFFAOYSA-N

      Shipping

      AMBIENT

      Short Term Storage

      +4°C

      Long Term Storage

      -20°C

      Handling Advice

      Protect from light and moisture.

      Use / Stability

      Stable for at least 2 years after receipt when stored at -20°C.

      Hazard statements

      H302

      GHS Symbol

      GHS07

      Signal word

      Warning

      Transportation

      Not dangerous goods

      References

      (1) W.S. Kim et al.: Anal. Chem. 78(6), 1859 (2006), (2) M.M. Knight et al.: Am. J. Physiol. 284(4, Pt. 1), C1083 (2003), (3) H. Kojima et al.: Adv. Mat. 12(10), 763 (2002), (4) H. Kojima et al.: Angew. Chem. 111( 21), 3419 (1999)

      InChi

      InChI=1S/C21H14F2N2O5/c1-25-13-3-2-8(19(20(13)24)21(28)29)18-9-4-11(22)14(26)6-16(9)30-17-7-15(27)12(23)5-10(17)18/h2-7,25-26H,24H2,1H3,(H,28,29)

      Quantity

      1 mg, 5 mg, Bulk

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