Specifications
- Purity
- ≥98% (HPLC)
- Identity
- 1H-NMR
- Appearance
- Orange powder
Properties
- Fluorescence
- λex 495 nm, λem 515 nm
- Solvents
- DMSO
Available from stock
DAF-FM
- SKU
- A0023
Category: Fluorescent Detection
- Synonyms
- 3-Amino-4-(N-methylamino)-2', 7'-difluorofluorescein
- 254109-20-1
- CAS-Number
- C21H14F2N2O5
- Molecular Formula
- 412.34
- Molecular Weight
- Long Term Storage
- -20°C
- Use / Stability
- Stable for at least 2 years after receipt when stored at -20°C.
- Handling Advice
- Protect from light and moisture.
- Short Term Storage
- +4°C
- Shipping
- AMBIENT
- Transportation
- Not dangerous goods
- Signal word
- Warning
- GHS Symbol
- GHS07
- Hazard statements
- H302
- Description
- DAF-FM is an important reagent for quantitating low concentrations of nitric oxide in solution. This compound is essentially nonfluorescent until it reacts with NO to form a fluorescent benzotrizole. With excitation/emission maxima of 495/515 nm, DAF-FM can be detected using any instrument that can be used to detect fluorescein.
- InChi
- InChI=1S/C21H14F2N2O5/c1-25-13-3-2-8(19(20(13)24)21(28)29)18-9-4-11(22)14(26)6-16(9)30-17-7-15(27)12(23)5-10(17)18/h2-7,25-26H,24H2,1H3,(H,28,29)
- References
- (1) W.S. Kim et al.: Anal. Chem. 78(6), 1859 (2006) , (2) M.M. Knight et al.: Am. J. Physiol. 284(4, Pt. 1), C1083 (2003) , (3) H. Kojima et al.: Adv. Mat. 12(10), 763 (2002) , (4) H. Kojima et al.: Angew. Chem. 111( 21), 3419 (1999)
- InChi Key
- BJTLSPOVXMBXRZ-UHFFFAOYSA-N
- Smiles
- CNC1=C(N)C(C(O)=O)=C(C=C1)C1=C2C=C(F)C(=O)C=C2OC2=C1C=C(F)C(O)=C2
DAF-FM is an important reagent for quantitating low concentrations of nitric oxide in solution. This compound is essentially nonfluorescent until it reacts with NO to form a fluorescent benzotrizole. With excitation/emission maxima of 495/515 nm, DAF-FM can be detected using any instrument that can be used to detect fluorescein.
Additional information
| Synonyms | 3-Amino-4-(N-methylamino)-2', 7'-difluorofluorescein |
|---|---|
| Purity | ≥98% (HPLC) |
| Appearance | Orange powder |
| CAS-Number | 254109-20-1 |
| Molecular Formula | C21H14F2N2O5 |
| Molecular Weight | 412.34 |
| Identity | 1H-NMR |
| Solvents | DMSO |
| Fluorescence | λex 495 nm, λem 515 nm |
| Smiles | CNC1=C(N)C(C(O)=O)=C(C=C1)C1=C2C=C(F)C(=O)C=C2OC2=C1C=C(F)C(O)=C2 |
| InChi Key | BJTLSPOVXMBXRZ-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Hazard statements | H302 |
| GHS Symbol | GHS07 |
| Signal word | Warning |
| Transportation | Not dangerous goods |
| References | (1) W.S. Kim et al.: Anal. Chem. 78(6), 1859 (2006), (2) M.M. Knight et al.: Am. J. Physiol. 284(4, Pt. 1), C1083 (2003), (3) H. Kojima et al.: Adv. Mat. 12(10), 763 (2002), (4) H. Kojima et al.: Angew. Chem. 111( 21), 3419 (1999) |
| InChi | InChI=1S/C21H14F2N2O5/c1-25-13-3-2-8(19(20(13)24)21(28)29)18-9-4-11(22)14(26)6-16(9)30-17-7-15(27)12(23)5-10(17)18/h2-7,25-26H,24H2,1H3,(H,28,29) |
| Quantity | 1 mg, 5 mg, Bulk |
