- Synonyms: 3-Amino-4-(N-methylamino)-2', 7'-difluorofluorescein
- Purity: ≥98% (HPLC)
- Appearance: Orange powder.
- CAS-Number: 254109-20-1
- Molecular Formula: C21H14F2N2O5
- Molecular Weight: 412.34
- Identity: 1H-NMR
- Solvents: DMSO
- Fluorescence: λex 495 nm; λem 515 nm
- Smiles: CNC1=C(N)C(C(O)=O)=C(C=C1)C1=C2C=C(F)C(=O)C=C2OC2=C1C=C(F)C(O)=C2
- InChi: InChI=1S/C21H14F2N2O5/c1-25-13-3-2-8(19(20(13)24)21(28)29)18-9-4-11(22)14(26)6-16(9)30-17-7-15(27)12(23)5-10(17)18/h2-7,25-26H,24H2,1H3,(H,28,29)
- InChi Key: BJTLSPOVXMBXRZ-UHFFFAOYSA-N
Available from stock
DAF-FM
SKU: A0023
Category: Fluorescent Detection
- Shipping: AMBIENT
- Short Term Storage: +4°C
- Long Term Storage: -20°C
- Handling Advice: Protect from light and moisture.
- Use / Stability: Stable for at least 2 years after receipt when stored at -20°C.
- Description: DAF-FM is an important reagent for quantitating low concentrations of nitric oxide in solution. This compound is essentially nonfluorescent until it reacts with NO to form a fluorescent benzotrizole. With excitation/emission maxima of 495/515 nm, DAF-FM can be detected using any instrument that can be used to detect fluorescein.
- References: (1) W.S. Kim et al.; Anal. Chem. 78(6), 1859 (2006), (2) M.M. Knight et al.; Am. J. Physiol. 284(4, Pt. 1), C1083 (2003), (3) H. Kojima et al.; Adv. Mat. 12(10), 763 (2002), (4) H. Kojima et al.; Angew. Chem. 111( 21), 3419 (1999)
DAF-FM is an important reagent for quantitating low concentrations of nitric oxide in solution. This compound is essentially nonfluorescent until it reacts with NO to form a fluorescent benzotrizole. With excitation/emission maxima of 495/515 nm, DAF-FM can be detected using any instrument that can be used to detect fluorescein.
Additional information
| Identity | 1H-NMR |
|---|---|
| Handling Advice | Protect from light and moisture. |
| Availability | 1 |
| CAS-Number | 254109-20-1 |
| Long Term Storage | -20°C |
| InChi Key | BJTLSPOVXMBXRZ-UHFFFAOYSA-N |
| Quantity | 1 mg, 5 mg, Bulk |
| Appearance | Orange powder. |
| InChi | InChI=1S/C21H14F2N2O5/c1-25-13-3-2-8(19(20(13)24)21(28)29)18-9-4-11(22)14(26)6-16(9)30-17-7-15(27)12(23)5-10(17)18/h2-7,25-26H,24H2,1H3,(H,28,29) |
| Fluorescence | λex 495 nm; λem 515 nm |
| Molecular Formula | |
| Short Term Storage | +4°C |
| Purity | |
| Pricetable | |
| References | (1) W.S. Kim et al.; Anal. Chem. 78(6), 1859 (2006), (2) M.M. Knight et al.; Am. J. Physiol. 284(4, Pt. 1), C1083 (2003), (3) H. Kojima et al.; Adv. Mat. 12(10), 763 (2002), (4) H. Kojima et al.; Angew. Chem. 111( 21), 3419 (1999) |
| Smiles | CNC1=C(N)C(C(O)=O)=C(C=C1)C1=C2C=C(F)C(=O)C=C2OC2=C1C=C(F)C(O)=C2 |
| Use / Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Molecular Weight | |
| Solvents | DMSO |
| Synonyms | 3-Amino-4-(N-methylamino)-2', 7'-difluorofluorescein |
| Shipping | AMBIENT |
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