Specifications
- Purity
- ≥95% (HPLC)
- Appearance
- Dark violet powder
- Identity
- 1H-NMR
Properties
- Solvents
- water, ethanol (slightly), DMSO (slightly), methanol (slightly)
- Melting Point
- 242-244°C
- Fluorescence
- λex 480 nm, λem 605 nm in water
Available from stock
Ethidium bromide
- SKU
- E0005
Categories: Fluorescent Detection, Fluorescent Detection
- Synonyms
- 3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide , EtBr , Homidium bromide
- 1239-45-8
- CAS-Number
- C21H20BrN3
- Molecular Formula
- 394.31
- Molecular Weight
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
- Shipping
- AMBIENT
- Short Term Storage
- RT
- Long Term Storage
- +4°C
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- This product should not be used if colour has changed or if there are
- Hazard statements
- H302-H330-H341
- Precautionary statements
- P260-P281-P284-P310
- GHS Symbol
- GHS06+GHS08
- Signal word
- Danger
- RIDADR
- UN2811PIH
- Transportation
- Packing Group I
- Description
- "Ethidium bromide (EtBr) is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 µg/ml). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. Ethidium bromide intercalates double-stranded DNA and RNA and acts as a frameshift mutagen. It confers a deep red stain to DNA by acting as an intercalating agent between the starch bases. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase. It can be used in conjunction with acridine orange to differentiate between viable, apoptotic and necrotic cells. Has been used in the past as an anti-parasitic and anti-microbial agent and described as an uncoupler of oxidative phosphorylation. Has also been used to stain mitochondrial DNA.
- Smiles
- CC[N+]1=C2C=C(C=CC2=C3C=CC(=CC3=C1C4=CC=CC=C4)N)N.[Br-]
- InChi Key
- ZMMJGEGLRURXTF-UHFFFAOYSA-N
“Ethidium bromide (EtBr) is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 µg/ml). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. Ethidium bromide intercalates double-stranded DNA and RNA and acts as a frameshift mutagen. It confers a deep red stain to DNA by acting as an intercalating agent between the starch bases. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase. It can be used in conjunction with acridine orange to differentiate between viable, apoptotic and necrotic cells. Has been used in the past as an anti-parasitic and anti-microbial agent and described as an uncoupler of oxidative phosphorylation. Has also been used to stain mitochondrial DNA.
Additional information
| Synonyms | 3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide, EtBr, Homidium bromide |
|---|---|
| Purity | ≥95% (HPLC) |
| Appearance | Dark violet powder |
| CAS-Number | 1239-45-8 |
| Molecular Formula | C21H20BrN3 |
| Molecular Weight | 394.31 |
| Identity | 1H-NMR |
| Solvents | water, ethanol (slightly), DMSO (slightly), methanol (slightly) |
| Melting Point | 242-244°C |
| Fluorescence | λex 480 nm, λem 605 nm in water |
| Smiles | CC[N+]1=C2C=C(C=CC2=C3C=CC(=CC3=C1C4=CC=CC=C4)N)N.[Br-] |
| InChi Key | ZMMJGEGLRURXTF-UHFFFAOYSA-N |
| Shipping | AMBIENT |
| Short Term Storage | RT |
| Long Term Storage | +4°C |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | This product should not be used if colour has changed or if there are |
| Hazard statements | H302-H330-H341 |
| Precautionary statements | P260-P281-P284-P310 |
| GHS Symbol | GHS06+GHS08 |
| Signal word | Danger |
| RIDADR | UN2811PIH |
| Transportation | Packing Group I |
| Quantity | 1 g |
