Available from stock

Fluorescamine

SKU: F0003

Categories: Fluorescent Detection, Fluorescent Detection

Synonyms: 1'(3'H)-isobenzofuran]-3,3'-dione, 4-phenyl-spiro[furan-2(3H) , 4-Phenylspiro[furan-2(3H),1'-phthalan]-3,3'-dione , Fluram; 38183-12-9
CAS-Number: C₁₇H₁₀O₄
Molecular Formula: 278.26
Molecular Weight

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Product Data
Handling
Safety Information
Details

Specifications

Purity: ≥98% (TLC)
Appearance: Off-white to yellow powder
Identity: 1H-NMR

Properties

Solvents: acetone (50 mg/ml), water (partly), ethanol, chloroform, DMSO, acetonitrile
Melting Point: 153-157 °C (lit.)
UV / Vis: λex 234 nm, λex 390 nm, λem 475 nm in 0.5 M borate pH 8.5 (after derivatization with L-leuc

Shipping: AMBIENT
Short Term Storage: RT
Long Term Storage: RT
Handling Advice: Protect from light and moisture.
Use / Stability: Stable for at least 2 years after receipt when stored at RT.

Transportation: Not dangerous goods

Description: A non-fluorescent reagent that reacts readily under mild conditions with primary amino groups in amino acids and peptides to form stable and highly fluorescent derivatives. Low background due to hydrolysis. Useful in fluorometric assays of amino acids, protein, and proteolytic enzymes. Effectively blocks newly generated amino termini in protein sequence analysis by the Beckman automated sequencer. Spectral data: Exc. 390nm/Em. 475 nm in 0.5 M borate buffer, pH 8.5, after derivatization with L-leucine.
Smiles: O=C1C(C2=CC=CC=C2)=COC31OC(C4=CC=CC=C43)=O
InChi Key: ZFKJVJIDPQDDFY-UHFFFAOYSA-N
References: (1) S. Udenfriend, et al.: Science 178, 871 (1972) , (2) M. Weigele, et al.: JACS 94, 5927 (1972) , (3) S. Stein, et al.: Arch. Biochem. Biophys. 155, 202 (1973) , (4) P. Bohlen, et al.: Arch. Biochem. Biophys. 163, 390 (1974) , (5) C.Y. Lai, et al.: Meth. Enzymol. 47, 236 (1977) , (6) K. Sogowa & K.J. Takahaski: Biochem. 83, 1783 (1978) , (7) A.S. Bhown, et al.: Anal. Biochem. 112, 158 (1981)
InChi: InChI=1S/C17H10O4/c18-15-13(11-6-2-1-3-7-11)10-20-17(15)14-9-5-4-8-12(14)16(19)21-17/h1-10H

A non-fluorescent reagent that reacts readily under mild conditions with primary amino groups in amino acids and peptides to form stable and highly fluorescent derivatives. Low background due to hydrolysis. Useful in fluorometric assays of amino acids, protein, and proteolytic enzymes. Effectively blocks newly generated amino termini in protein sequence analysis by the Beckman automated sequencer. Spectral data: Exc. 390nm/Em. 475 nm in 0.5 M borate buffer, pH 8.5, after derivatization with L-leucine.

SKU: F0003 Categories: Fluorescent Detection, Fluorescent Detection

Additional information

Additional information

Synonyms	1'(3'H)-isobenzofuran]-3,3'-dione, 4-phenyl-spiro[furan-2(3H), 4-Phenylspiro[furan-2(3H),1'-phthalan]-3,3'-dione, Fluram
Purity	≥98% (TLC)
Appearance	Off-white to yellow powder
CAS-Number	38183-12-9
Molecular Formula	C17H10O4
Molecular Weight	278.26
Identity	1H-NMR
Solvents	acetone (50 mg/ml), water (partly), ethanol, chloroform, DMSO, acetonitrile
Melting Point	153-157 °C (lit.)
UV / Vis	λex 234 nm, λex 390 nm, λem 475 nm in 0.5 M borate pH 8.5 (after derivatization with L-leuc
Smiles	O=C1C(C2=CC=CC=C2)=COC31OC(C4=CC=CC=C43)=O
InChi Key	ZFKJVJIDPQDDFY-UHFFFAOYSA-N
Shipping	AMBIENT
Short Term Storage	RT
Long Term Storage	RT
Handling Advice	Protect from light and moisture.
Use / Stability	Stable for at least 2 years after receipt when stored at RT.
Transportation	Not dangerous goods
References	(1) S. Udenfriend, et al.: Science 178, 871 (1972), (2) M. Weigele, et al.: JACS 94, 5927 (1972), (3) S. Stein, et al.: Arch. Biochem. Biophys. 155, 202 (1973), (4) P. Bohlen, et al.: Arch. Biochem. Biophys. 163, 390 (1974), (5) C.Y. Lai, et al.: Meth. Enzymol. 47, 236 (1977), (6) K. Sogowa & K.J. Takahaski: Biochem. 83, 1783 (1978), (7) A.S. Bhown, et al.: Anal. Biochem. 112, 158 (1981)
InChi	InChI=1S/C17H10O4/c18-15-13(11-6-2-1-3-7-11)10-20-17(15)14-9-5-4-8-12(14)16(19)21-17/h1-10H
Quantity	100 mg, 250 mg, 1 g, Bulk